Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Coordination of tRNA nuclear export with processing of tRNA.
Lipowsky G
,
Bischoff FR
,
Izaurralde E
,
Kutay U
,
Schäfer S
,
Gross HJ
,
Beier H
,
Görlich D
.
???displayArticle.abstract???
Eukaryotic tRNAs are synthesized in the nucleus and need to be exported to the cytoplasm where they function in translation. tRNA export is mediated by exportin-t, which binds tRNA directly and with high affinity. tRNAs are initially synthesized as precursor molecules. Maturation to functional tRNA takes place in the nucleus, precedes export, and includes trimming of the 5' and 3' ends, posttranscriptional addition of the 3' CCA end, nucleoside modifications, and in some cases splicing. Here we address the question of how tRNA maturation is coordinated with export and thus how cytoplasmic accumulation of inactive maturation intermediates is avoided. This could, in principle, be achieved by nuclear retention of immature tRNA or by selective export of the fully mature form. We show that exportin-t has a strong preference for tRNA with correctly processed 5' and 3' ends and nucleoside modification. tRNA recognition by exportin-t can thus be considered as a quality control mechanism for these maturation steps prior to tRNA export. Surprisingly however, exportin-t can efficiently bind unspliced tRNA and intron-containing tRNA is exported when the rate of splicing is slow. During characterization of the exportin-t/tRNA interaction we found that exportin-t recognizes features in the tRNA that are conserved between prokaryotic and eukaryotic tRNAs. Our data suggest that correct tRNA shape, the 5' and 3' terminal ends, and the TpsiC loop are critical for exportin-t binding.
Abelson,
tRNA splicing.
1998,
Pubmed
Akama,
Characterization of nuclear tRNA(Tyr) introns: their evolution from red algae to higher plants.
1997,
Pubmed
Arts,
The role of exportin-t in selective nuclear export of mature tRNAs.
1998,
Pubmed
,
Xenbase
Arts,
Identification of a nuclear export receptor for tRNA.
1998,
Pubmed
,
Xenbase
Belfort,
Another bridge between kingdoms: tRNA splicing in archaea and eukaryotes.
1997,
Pubmed
Bertrand,
Nucleolar localization of early tRNA processing.
1998,
Pubmed
Bischoff,
Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.
1995,
Pubmed
Boelens,
Nuclear retention of RNA as a mechanism for localization.
1995,
Pubmed
,
Xenbase
Dahlberg,
Functions of the GTPase Ran in RNA export from the nucleus.
1998,
Pubmed
De Robertis,
Intranuclear location of the tRNA splicing enzymes.
1981,
Pubmed
,
Xenbase
Floer,
The nuclear transport factor karyopherin beta binds stoichiometrically to Ran-GTP and inhibits the Ran GTPase activating protein.
1996,
Pubmed
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Görlich,
Identification of different roles for RanGDP and RanGTP in nuclear protein import.
1996,
Pubmed
,
Xenbase
Hellmuth,
Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA.
1998,
Pubmed
Hopper,
Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs.
1980,
Pubmed
Hurt,
Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing.
1987,
Pubmed
Izaurralde,
The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus.
1997,
Pubmed
Izaurralde,
Transport of macromolecules between the nucleus and the cytoplasm.
1998,
Pubmed
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Kutay,
Identification of a tRNA-specific nuclear export receptor.
1998,
Pubmed
,
Xenbase
Kutay,
Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor.
1997,
Pubmed
,
Xenbase
Lee,
Transfer RNA splicing in Saccharomyces cerevisiae. Secondary and tertiary structures of the substrates.
1985,
Pubmed
Lee,
Mutants of Escherichia coli initiator tRNA that suppress amber codons in Saccharomyces cerevisiae and are aminoacylated with tyrosine by yeast extracts.
1991,
Pubmed
Lund,
Proofreading and aminoacylation of tRNAs before export from the nucleus.
1998,
Pubmed
,
Xenbase
Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed
Melton,
Order and intracellular location of the events involved in the maturation of a spliced tRNA.
1980,
Pubmed
,
Xenbase
Nishikura,
RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in the spliced transfer RNA.
1981,
Pubmed
,
Xenbase
Pederson,
The plurifunctional nucleolus.
1998,
Pubmed
Quinn,
Species-specific microhelix aminoacylation by a eukaryotic pathogen tRNA synthetase dependent on a single base pair.
1995,
Pubmed
Raba,
Nucleotide sequence of three isoaccepting lysine tRNAs from rabbit liver and SV40-transformed mouse fibroblasts.
1979,
Pubmed
Sarkar,
tRNA nuclear export in saccharomyces cerevisiae: in situ hybridization analysis.
1998,
Pubmed
Schäfer,
Mammalian tRNA(Lys)3 and pre-tRNA(Lys)3 variants as primers and inhibitors of viral cDNA synthesis by HIV reverse transcriptase in vitro.
1997,
Pubmed
Simos,
Nuclear pore proteins are involved in the biogenesis of functional tRNA.
1996,
Pubmed
Sprinzl,
Compilation of tRNA sequences and sequences of tRNA genes.
1989,
Pubmed
Stade,
Exportin 1 (Crm1p) is an essential nuclear export factor.
1997,
Pubmed
Stange,
A cell-free plant extract for accurate pre-tRNA processing, splicing and modification.
1987,
Pubmed
,
Xenbase
Swerdlow,
Structure of intron-containing tRNA precursors. Analysis of solution conformation using chemical and enzymatic probes.
1984,
Pubmed
Tobian,
tRNA nuclear transport: defining the critical regions of human tRNAimet by point mutagenesis.
1985,
Pubmed
,
Xenbase
Weber,
Another heritage from the RNA world: self-excision of intron sequence from nuclear pre-tRNAs.
1996,
Pubmed
Zasloff,
TRNA precursor transcribed from a mutant human gene inserted into a SV40 vector is processed incorrectly.
1982,
Pubmed
Zasloff,
Impaired nuclear transport of a human variant tRNAiMet.
1982,
Pubmed
,
Xenbase
Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
1983,
Pubmed
,
Xenbase
Zerfass,
Pseudouridine in the anticodon G psi A of plant cytoplasmic tRNA(Tyr) is required for UAG and UAA suppression in the TMV-specific context.
1992,
Pubmed
