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Evidence for dimerization of dimers in K+ channel assembly.
Tu L
,
Deutsch C
.
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Voltage-gated K+ channels are tetrameric, but how the four subunits assemble is not known. We analyzed inactivation kinetics and peak current levels elicited for a variety of wild-type and mutant Kv1.3 subunits, expressed singly, in combination, and as tandem constructs, to show that 1) the dominant pathway involves a dimerization of dimers, and 2) dimer-dimer interaction may involve interaction sites that differ from those involved in monomer-monomer association. Moreover, using nondenaturing gel electrophoresis, we detected dimers and tetramers, but not trimers, in the translation reaction of Kv1.3 monomers.
Babila,
Assembly of mammalian voltage-gated potassium channels: evidence for an important role of the first transmembrane segment.
1994, Pubmed,
Xenbase
Babila,
Assembly of mammalian voltage-gated potassium channels: evidence for an important role of the first transmembrane segment.
1994,
Pubmed
,
Xenbase
Blount,
Assembly intermediates of the mouse muscle nicotinic acetylcholine receptor in stably transfected fibroblasts.
1990,
Pubmed
Boland,
Cysteines in the Shaker K+ channel are not essential for channel activity or zinc modulation.
1994,
Pubmed
,
Xenbase
Busch,
Current inactivation involves a histidine residue in the pore of the rat lymphocyte potassium channel RGK5.
1991,
Pubmed
,
Xenbase
Chahine,
Lidocaine block of human heart sodium channels expressed in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Corey,
Number and stoichiometry of subunits in the native atrial G-protein-gated K+ channel, IKACh.
1998,
Pubmed
Cortes,
Structural dynamics of the Streptomyces lividans K+ channel (SKC1): oligomeric stoichiometry and stability.
1997,
Pubmed
,
Xenbase
Deal,
The brain Kv1.1 potassium channel: in vitro and in vivo studies on subunit assembly and posttranslational processing.
1994,
Pubmed
Doms,
Folding and assembly of viral membrane proteins.
1993,
Pubmed
Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed
Green,
Acetylcholine receptor assembly: subunit folding and oligomerization occur sequentially.
1993,
Pubmed
Gu,
Assembly of the mammalian muscle acetylcholine receptor in transfected COS cells.
1991,
Pubmed
Heginbotham,
The aromatic binding site for tetraethylammonium ion on potassium channels.
1992,
Pubmed
Helenius,
The endoplasmic reticulum as a protein-folding compartment.
1992,
Pubmed
Holsinger,
Influenza virus M2 integral membrane protein is a homotetramer stabilized by formation of disulfide bonds.
1991,
Pubmed
Jegla,
A novel subunit for shal K+ channels radically alters activation and inactivation.
1997,
Pubmed
Li,
Images of purified Shaker potassium channels.
1994,
Pubmed
,
Xenbase
Li,
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
1992,
Pubmed
Liu,
Subunit stoichiometry of cyclic nucleotide-gated channels and effects of subunit order on channel function.
1996,
Pubmed
Liu,
Constraining ligand-binding site stoichiometry suggests that a cyclic nucleotide-gated channel is composed of two functional dimers.
1998,
Pubmed
MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase
Manolios,
Pairwise, cooperative and inhibitory interactions describe the assembly and probable structure of the T-cell antigen receptor.
1991,
Pubmed
McCormack,
Tandem linkage of Shaker K+ channel subunits does not ensure the stoichiometry of expressed channels.
1992,
Pubmed
,
Xenbase
Nagaya,
Potassium channel alpha and beta subunits assemble in the endoplasmic reticulum.
1997,
Pubmed
Ogielska,
Cooperative subunit interactions in C-type inactivation of K channels.
1995,
Pubmed
,
Xenbase
Panyi,
C-type inactivation of a voltage-gated K+ channel occurs by a cooperative mechanism.
1995,
Pubmed
Panyi,
Assembly and suppression of endogenous Kv1.3 channels in human T cells.
1996,
Pubmed
Root,
Two identical noninteracting sites in an ion channel revealed by proton transfer.
1994,
Pubmed
,
Xenbase
Saedi,
Assembly of Torpedo acetylcholine receptors in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Sakaguchi,
The active oligomeric state of the minimalistic influenza virus M2 ion channel is a tetramer.
1997,
Pubmed
Schulteis,
Intersubunit interaction between amino- and carboxyl-terminal cysteine residues in tetrameric shaker K+ channels.
1996,
Pubmed
,
Xenbase
Schulteis,
Conserved cysteine residues in the shaker K+ channel are not linked by a disulfide bond.
1995,
Pubmed
,
Xenbase
Shen,
Deletion analysis of K+ channel assembly.
1993,
Pubmed
,
Xenbase
Sheng,
Evidence for interaction between transmembrane segments in assembly of Kv1.3.
1997,
Pubmed
,
Xenbase
Tu,
Truncated K+ channel DNA sequences specifically suppress lymphocyte K+ channel gene expression.
1995,
Pubmed
,
Xenbase
Tu,
Voltage-gated K+ channels contain multiple intersubunit association sites.
1996,
Pubmed
,
Xenbase
Xu,
Assembly of voltage-gated potassium channels. Conserved hydrophilic motifs determine subfamily-specific interactions between the alpha-subunits.
1995,
Pubmed
