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XB-ART-1349
J Cell Biol. September 26, 2005; 170 (7): 1047-55.

Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis.

Kinoshita K , Noetzel TL , Pelletier L , Mechtler K , Drechsel DN , Schwager A , Lee M , Raff JW , Hyman AA .


Abstract
Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3-XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A-dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.

PubMed ID: 16172205
PMC ID: PMC2171544
Article link: J Cell Biol.

Genes referenced: aurka ccnb1.2 ckap5 kif2c tacc3
Antibodies referenced:
Morpholinos referenced:
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