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XB-ART-138
Dev Biol August 15, 2006; 296 (2): 485-98.

Neogenin interacts with RGMa and netrin-1 to guide axons within the embryonic vertebrate forebrain.



Abstract
In the embryonic forebrain, pioneer axons establish a simple topography of dorsoventral and longitudinal tracts. The cues used by these axons during the initial formation of the axon scaffold remain largely unknown. We have investigated the axon guidance role of Neogenin, a member of the immunoglobulin (Ig) superfamily that binds to the chemoattractive ligand Netrin-1, as well as to the chemorepulsive ligand repulsive guidance molecule (RGMa). Here, we show strong expression of Neogenin and both of its putative ligands in the developing Xenopus forebrain. Neogenin loss-of-function mutants revealed that this receptor was essential for axon guidance in an early forming dorsoventral brain pathway. Similar mutant phenotypes were also observed following loss of either RGMa or Netrin-1. Simultaneous partial knock downs of these molecules revealed dosage-sensitive interactions and confirmed that these receptors and ligands were acting in the same pathway. The results provide the first evidence that Neogenin acts as an axon guidance molecule in vivo and support a model whereby Neogenin-expressing axons respond to a combination of attractive and repulsive cues as they navigate their ventral trajectory.

PubMed ID: 16836993
Article link: Dev Biol


Species referenced: Xenopus laevis
Genes referenced: ncam1 neo1 ntn1 pc.1 rgma sema3a sult2a1 tuba4b
Antibodies: Ncam1 Ab4 Tuba4b Ab11
Morpholinos: neo1 MO2 neo1 MO4 ntn1 MO1 rgma MO1 rgma MO2 sema3a MO1


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