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Nature October 8, 1998; 395 (6702): 608-12.

The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors.

Roose J , Molenaar M , Peterson J , Hurenkamp J , Brantjes H , Moerer P , van de Wetering M , Destrée O , Clevers H .

Tcf/Lef transcription factors mediate signalling from Wingless/Wnt proteins by recruiting Armadillo/beta-catenin as a transcriptional co-activator. However, studies of Drosophila, Xenopus and Caenorhabditis elegans have indicated that Tcf factors may also be transcriptional repressors. Here we show that Tcf factors physically interact with members of the Groucho family of transcriptional repressors. In transient transfection assays, the Xenopus Groucho homologue XGrg-4 inhibited activation of transcription of synthetic Tcf reporter genes. In contrast, the naturally truncated Groucho-family member XGrg-5 enhanced transcriptional activation. Injection of XGrg-4 into Xenopus embryos repressed transcription of Siamois and Xnr-3, endogenous targets of beta-catenin-Tcf. Dorsal injection of XGrg-4 had a ventralizing effect on Xenopus embryos. Secondary-axis formation induced by a dominant-positive Armadillo-Tcf fusion protein was inhibited by XGrg-4 and enhanced by XGrg-5. These data indicate that expression of Tcf target genes is regulated by a balance between Armadillo and Groucho.

PubMed ID: 9783587
Article link: Nature

Species referenced: Xenopus
Genes referenced: nodal3.1 sia1 tcf7l1 tle4 tle5