XB-ART-14258Mech Dev July 1, 1998; 75 (1-2): 155-8.
Xenopus eomesodermin is expressed in neural differentiation.
Our initial description of the Xenopus gene Eomesodermin (Eomes) indicated that it is expressed largely if not entirely in mesodermal cells of gastrula stage embryos. A more detailed examination, described here, shows that it continues to be expressed in the most anterior (future head) mesoderm during gastrula and neurula stages. However, during tail-bud stages, Eomes expression is re-born in the most anterior part of the brain, becoming strongly transcribed in the olfactory region of the telencephalon. This later Eomes expression marks a very localized region of the forebrain distinct from that of Otx-2, anterior to that of En-1 and overlapping that of Sox-3.
PubMed ID: 9739133
Article link: Mech Dev
Genes referenced: en1 en2 eomes neurod1 otx2 sox3 tub tubb2b
Article Images: [+] show captions
|Fig. 2. Eomes and other gene expressions in tailbud embryos. (A) Cleared whole-mount in situ hybridization, stage 28; (G), whole-mount stained for Eomes; (H), horizontal section (in plane of line) through whole-mounts, stage 28; (I) vertical section, 50 mm to one side of the midline, in situ hybridized to Eomes mRNA, stage 23. Gene probes shown above and below plates. En, engrailed-1; N-tub, N-tubulin; st, stage|
|Fig. 3. Eomes expression, by in situ hybridization to a dissected brain from a stage 51 tadpole, is seen most strongly in the olfactory region of the anterior forebrain. (A) dissected brain; (B) ventral and dorsal view of telencephalon, dorsal view of hindbrain; (C) vertical section to one side of midline of Eomes- stained brain; (D) cresyl violet stain of section shown in (C). Abbreviations: aop, area olfactoria primitiva (arrows); e, epiphysis; c, Cb cerebellum; VA, ventral anterior; DA, dorsal anterior. Surface pigment cells appear as irregular black dots; Eomes staining is purple.|