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XB-ART-1440
J Biol Chem. October 28, 2005; 280 (43): Identification and functional characterization of a voltage-gated chloride channel and its novel splice variant in taste bud cells.
Huang L
,
Cao J
,
Wang H
,
Vo LA
,
Brand JG
.
Abstract Taste bud cells are epithelial cells with neuronal properties. Voltage-dependent ion channels have been physiologically described in these cells. Here, we report the molecular identification and functional characterization of a voltage-gated chloride channel (ClC-4) and its novel splice variant (ClC-4A) from taste bud cells. ClC-4A skipped an exon near its 5''-end, incurring the loss of 60 amino acids at the N terminus. In situ hybridization and immunohistochemistry localized these two channels'' transcripts and proteins to a subset of taste bud cells. Electrophysiological recordings of the heterologously expressed channels in Xenopus oocytes showed that ClC-4 and ClC-4A have opposite sensitivity to pH and unique ion selectivity. The chloride channel blockers niflumic acid and 5-nitro-2-(3-phenylpropylamino)benzoic acid had a slight or no inhibitory effect on the conductance of ClC-4, but both blockers inhibited ClC-4A, suggesting that ClC-4A is a candidate channel for an acid-induced 5-nitro-2-(3-phenylpropylamino)benzoic acid-sensitive current. Furthermore, these two channels may play a role in bitter-, sweet-, and umami-mediated taste transmission by regulating transmitter uptake into synaptic vesicles.Pubmed Id: 16129671 Article link: J Biol Chem.Grant support:
DC05154 NIDCD NIH HHS, R03 DC005154-04 NIDCD NIH HHS Genes referenced: Antibodies referenced: |
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