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XB-ART-14452
Mol Cell July 1, 1998; 2 (1): 121-7.

Characterization of human FAST-1, a TGF beta and activin signal transducer.

Zhou S , Zawel L , Lengauer C , Kinzler KW , Vogelstein B .


Abstract
We have identified a human homolog of the Xenopus forkhead activin signal transducer-1 (xFAST-1). Although significantly different in sequence from its Xenopus counterpart, hFAST-1 shared with xFAST-1 the ability to bind to human Smad2 and activate an activin response element (ARE). The hFAST-1-dependent activation of ARE was completely dependent on endogenous Smad4 and stimulation by a TGF beta-like ligand. The hFAST-1 protein was shown to bind to a novel DNA motif, TGT (G/T) (T/G)ATT, an exact copy of which was present within the ARE. A single copy of this motif could activate a reporter in a TGF beta-dependent fashion but only when an adjacent Smad-binding element was present in the construct. These data suggest that responses to TGF beta family members may be mediated by a DNA-binding complex formed by hFAST-1, hSmad2, and hSmad4.

PubMed ID: 9702198
Article link: Mol Cell
Grant support: [+]
Genes referenced: foxh1 smad2 smad4.1 smad4.2 tgfb1



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