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XB-ART-14572
Dev Dyn July 1, 1998; 212 (3): 335-45.

Conditions for fibronectin fibril formation in the early Xenopus embryo.



Abstract
Fibronectin fibril formation on a multilayered cohesive cell sheet is studied in the Xenopus embryo. In the blastula, secreted fibronectin accumulates in the blastocoel, where it associates with mucous material. At the onset of gastrulation, a fibrillar fibronectin matrix develops on the blastocoel roof. Cells engage in this process stochastically within a 2-hr period. Fibril network formation requires more than 60 microg/ml of fibronectin, but the timing of fibrillogenesis is not regulated through the availability of fibronectin. With the exception of a few isolated mesoderm cells, only the cells of the blastocoel roof are able to form fibronectin fibrils. However, this requires that cells are provided with a free surface and, at the same time, with lateral adhesive cell contacts, i.e. fibril assembly occurs only on the surface of cohesive cells aggregates. This explains the observed restriction of fibronectin matrix formation to the inner surface of the blastocoel roof in the embryo. In addition, a minimum blastocoel roof size is required for fibril formation.

PubMed ID: 9671937
Article link: Dev Dyn

Genes referenced: bcr cdh3 fn1
Antibodies: Fn1 Ab4


Article Images: [+] show captions


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