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Genes Dev
1998 Jul 01;1213:1986-97. doi: 10.1101/gad.12.13.1986.
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Identification of Xenopus SMC protein complexes required for sister chromatid cohesion.
Losada A
,
Hirano M
,
Hirano T
.
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The structural maintenance of chromosomes (SMC) family is a growing family of chromosomal ATPases. The founding class of SMC protein complexes, condensins, plays a central role in mitotic chromosome condensation. We report here a new class of SMC protein complexes containing XSMC1 and XSMC3, Xenopus homologs of yeast Smc1p and Smc3p, respectively. The protein complexes (termed cohesins) exist as two major forms with sedimentation coefficients of 9S and 14S. 9S cohesin is a heterodimer of XSMC1 and XSMC3, whereas 14S cohesin contains three additional subunits. One of them has been identified as a Xenopus homolog of the Schizosaccharomyces pombe Rad21p implicated in DNA repair and the Saccharomyces cerevisiae Scc1p/Mcd1p implicated in sister chromatid cohesion. 14S cohesin binds to interphase chromatin independently of DNA replication and dissociates from it at the onset of mitosis. Immunodepletion of cohesins during interphase causes defects in sister chromatid cohesion in subsequent mitosis, whereas condensation is unaffected. These results suggest that proper assembly of mitotic chromosomes is regulated by two distinct classes of SMC protein complexes, cohesins and condensins.
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9649503
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Benavente,
Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis.
1985, Pubmed,
Xenbase
Benavente,
Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Birkenbihl,
Cloning and characterization of rad21 an essential gene of Schizosaccharomyces pombe involved in DNA double-strand-break repair.
1992,
Pubmed
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Castaño,
Mitotic chromosome condensation in the rDNA requires TRF4 and DNA topoisomerase I in Saccharomyces cerevisiae.
1996,
Pubmed
,
Xenbase
Coleman,
The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts.
1996,
Pubmed
,
Xenbase
Giménez-Abián,
A postprophase topoisomerase II-dependent chromatid core separation step in the formation of metaphase chromosomes.
1995,
Pubmed
Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase
Guacci,
A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae.
1997,
Pubmed
Hirano,
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
1997,
Pubmed
,
Xenbase
Hirano,
Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extracts.
1993,
Pubmed
,
Xenbase
Hirano,
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.
1994,
Pubmed
,
Xenbase
Hirano,
The SMC family: from chromosome condensation to dosage compensation.
1995,
Pubmed
,
Xenbase
Holt,
An extragenic suppressor of the mitosis-defective bimD6 mutation of Aspergillus nidulans codes for a chromosome scaffold protein.
1996,
Pubmed
Hong,
Molecular characterization of neurally expressing genes in the para sodium channel gene cluster of drosophila.
1996,
Pubmed
Jessberger,
SMC proteins constitute two subunits of the mammalian recombination complex RC-1.
1996,
Pubmed
,
Xenbase
Kimura,
ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication for chromosome condensation.
1997,
Pubmed
,
Xenbase
Koshland,
Mitotic chromosome condensation.
1996,
Pubmed
Kubota,
Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor.
1995,
Pubmed
,
Xenbase
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
McKay,
Sequence conservation of the rad21 Schizosaccharomyces pombe DNA double-strand break repair gene in human and mouse.
1996,
Pubmed
Michaelis,
Cohesins: chromosomal proteins that prevent premature separation of sister chromatids.
1997,
Pubmed
Miyazaki,
Sister-chromatid cohesion in mitosis and meiosis.
1994,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Newport,
Disassembly of the nucleus in mitotic extracts: membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes.
1987,
Pubmed
,
Xenbase
Rocques,
The human SB1.8 gene (DXS423E) encodes a putative chromosome segregation protein conserved in lower eukaryotes and prokaryotes.
1995,
Pubmed
Romanowski,
The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin.
1996,
Pubmed
,
Xenbase
Rowles,
Interaction between the origin recognition complex and the replication licensing system in Xenopus.
1996,
Pubmed
,
Xenbase
Saitoh,
The SMC proteins and the coming of age of the chromosome scaffold hypothesis.
1995,
Pubmed
,
Xenbase
Saka,
Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis.
1994,
Pubmed
Shamu,
Sister chromatid separation in frog egg extracts requires DNA topoisomerase II activity during anaphase.
1992,
Pubmed
,
Xenbase
Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Strunnikov,
SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family.
1993,
Pubmed
Strunnikov,
SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.
1995,
Pubmed
Sutani,
DNA renaturation activity of the SMC complex implicated in chromosome condensation.
1997,
Pubmed
Yanagida,
Frontier questions about sister chromatid separation in anaphase.
1995,
Pubmed
,
Xenbase
