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Isoform-specific regulation of the Na+/Ca2+ exchanger in rat astrocytes and neurons by PKA.
He S
,
Ruknudin A
,
Bambrick LL
,
Lederer WJ
,
Schulze DH
.
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The Na+/Ca2+ exchanger is a major transporter of Ca2+ in neurons and glial cells. The Na+/Ca2+ exchanger gene NCX1 expresses tissue-specific isoforms of the Na+/Ca2+ exchanger, and the isoforms have been examined here quantitatively using primary cultures of astrocytes and neurons. We present a PCR-based quantitative method, quantitative end-labeled reverse transcription-PCR (QERT-PCR), to determine the relative amounts of the NCX1 isoforms present in these cells. Six exons (A, B, C, D, E, and F) are alternatively spliced to produce the known NCX1 isoforms. Three exon B-containing isoforms (BDEF, BDF, and BD) are the predominant transcripts in primary rat cortical astrocytes and in C6 glioma cells. In contrast, exon A-containing isoforms (ADF and AD) are the predominant transcripts in primary rat hippocampal neurons. Functional differences between full-length constructs of NCX1 containing either the astrocyte isoform BD or the neuron isoform AD were examined in a Xenopus oocyte expression system. Although both isoforms function normally, the activity of the AD isoform can be increased 39% by activation of protein kinase A (PKA), whereas that of the BD isoform is not affected. We conclude that specific NCX1 isoforms are expressed in distinct patterns in astrocytes and neurons. Furthermore, the activity of a neuronal (but not glial) isoform of the Na+/Ca2+ exchanger can be altered by the activation of the PKA pathway.
Bambrick,
Glutamate as a hippocampal neuron survival factor: an inherited defect in the trisomy 16 mouse.
1995, Pubmed
Bambrick,
Glutamate as a hippocampal neuron survival factor: an inherited defect in the trisomy 16 mouse.
1995,
Pubmed
Barnes,
Cloning of cardiac, kidney, and brain promoters of the feline ncx1 gene.
1997,
Pubmed
Bender,
Stimulation of calcium uptake in cultured astrocytes by hypoosmotic stress--effect of cyclic AMP.
1994,
Pubmed
Berridge,
The AM and FM of calcium signalling.
1997,
Pubmed
Bissell,
Characteristics of the rat C-6 glioma maintained in organ culture systems. Production of glial fibrillary acidic protein in the absence of gliofibrillogenesis.
1974,
Pubmed
Booher,
Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures.
1972,
Pubmed
Caroni,
The regulation of the Na+ -Ca2+ exchanger of heart sarcolemma.
1983,
Pubmed
Collins,
The giant cardiac membrane patch method: stimulation of outward Na(+)-Ca2+ exchange current by MgATP.
1992,
Pubmed
Cornell-Bell,
Glutamate induces calcium waves in cultured astrocytes: long-range glial signaling.
1990,
Pubmed
DiPolo,
A novel 13 kDa cytoplasmic soluble protein is required for the nucleotide (MgATP) modulation of the Na/Ca exchange in squid nerve fibers.
1997,
Pubmed
Finkbeiner,
Glial calcium.
1993,
Pubmed
Gabellini,
Expression of an active Na+/Ca2+ exchanger isoform lacking the six C-terminal transmembrane segments.
1996,
Pubmed
Gabellini,
An alternative splicing site modifies the carboxyl-terminal trans-membrane domains of the Na+/Ca2+ exchanger.
1995,
Pubmed
Goldin,
Maintenance of Xenopus laevis and oocyte injection.
1992,
Pubmed
,
Xenbase
Golovina,
Modulation of two functionally distinct Ca2+ stores in astrocytes: role of the plasmalemmal Na/Ca exchanger.
1996,
Pubmed
Haas,
Histamine may act through cyclic AMP on hippocampal neurones.
1985,
Pubmed
Iwamoto,
Phosphorylation-dependent regulation of cardiac Na+/Ca2+ exchanger via protein kinase C.
1996,
Pubmed
Kamei,
Histamine-induced depolarization and the cyclic AMP--protein kinase A system in isolated guinea pig adipocytes.
1992,
Pubmed
Kofuji,
Mutually exclusive and cassette exons underlie alternatively spliced isoforms of the Na/Ca exchanger.
1994,
Pubmed
Kofuji,
Na/Ca exchanger isoforms expressed in kidney.
1993,
Pubmed
Kofuji,
Expression of the Na-Ca exchanger in diverse tissues: a study using the cloned human cardiac Na-Ca exchanger.
1992,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Kumar,
The hormonal regulation of gene expression of glial markers: glutamine synthetase and glycerol phosphate dehydrogenase in primary cultures of rat brain and in C6 cell line.
1986,
Pubmed
Kuzhikandathil,
Transcription of the brain creatine kinase gene in glial cells is modulated by cyclic AMP-dependent protein kinase.
1994,
Pubmed
Lee,
Tissue-specific expression of Na(+)-Ca2+ exchanger isoforms.
1994,
Pubmed
Li,
Cloning of the NCX2 isoform of the plasma membrane Na(+)-Ca2+ exchanger.
1994,
Pubmed
,
Xenbase
Low,
Cloning of the rat heart Na(+)-Ca2+ exchanger and its functional expression in HeLa cells.
1993,
Pubmed
Madison,
Cyclic adenosine 3',5'-monophosphate mediates beta-receptor actions of noradrenaline in rat hippocampal pyramidal cells.
1986,
Pubmed
Nakasaki,
Cloning of the rat aortic smooth muscle Na+/Ca2+ exchanger and tissue-specific expression of isoforms.
1993,
Pubmed
Nedergaard,
Direct signaling from astrocytes to neurons in cultures of mammalian brain cells.
1994,
Pubmed
Nicoll,
Cloning of a third mammalian Na+-Ca2+ exchanger, NCX3.
1996,
Pubmed
,
Xenbase
Parpura,
Glutamate-mediated astrocyte-neuron signalling.
1994,
Pubmed
Pedarzani,
Dopamine modulates the slow Ca(2+)-activated K+ current IAHP via cyclic AMP-dependent protein kinase in hippocampal neurons.
1995,
Pubmed
Pedarzani,
PKA mediates the effects of monoamine transmitters on the K+ current underlying the slow spike frequency adaptation in hippocampal neurons.
1993,
Pubmed
Porter,
GFAP-positive hippocampal astrocytes in situ respond to glutamatergic neuroligands with increases in [Ca2+]i.
1995,
Pubmed
Quednau,
Tissue specificity and alternative splicing of the Na+/Ca2+ exchanger isoforms NCX1, NCX2, and NCX3 in rat.
1997,
Pubmed
Reuter,
Sodium-calcium exchange. Ins and outs of Ca2+ transport.
1991,
Pubmed
Ruknudin,
Na+/Ca2+ exchanger in Drosophila: cloning, expression, and transport differences.
1997,
Pubmed
,
Xenbase
Sakurai,
Immunochemical identification and differential phosphorylation of alternatively spliced forms of the alpha 1A subunit of brain calcium channels.
1995,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schell,
Inositol (1,4,5)-trisphosphate receptor: characterization of neuron-specific alternative splicing in rat brain and peripheral tissues.
1993,
Pubmed
Smith,
Activation of adenylyl cyclase downregulates sodium/calcium exchanger of arterial myocytes.
1995,
Pubmed
Smith,
Phorbol esters downregulate expression of the sodium/calcium exchanger in renal epithelial cells.
1995,
Pubmed
Takuma,
Role of Na(+)-Ca2+ exchanger in agonist-induced Ca2+ signaling in cultured rat astrocytes.
1996,
Pubmed
Torres,
Cyclic AMP and protein kinase A mediate 5-hydroxytryptamine type 4 receptor regulation of calcium-activated potassium current in adult hippocampal neurons.
1995,
Pubmed
Wang,
Quantitation of mRNA by the polymerase chain reaction.
1989,
Pubmed
White,
Mitochondria and Na+/Ca2+ exchange buffer glutamate-induced calcium loads in cultured cortical neurons.
1995,
Pubmed
