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Histone H1 reduces the frequency of initiation in Xenopus egg extract by limiting the assembly of prereplication complexes on sperm chromatin.
Lu ZH
,
Sittman DB
,
Romanowski P
,
Leno GH
.
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Somatic histone H1 reduces both the rate and extent of DNA replication in Xenopus egg extract. We show here that H1 inhibits replication directly by reducing the number of replication forks, but not the rate of fork progression, in Xenopus sperm nuclei. Density substitution experiments demonstrate that those forks that are active in H1 nuclei elongate to form large tracts of fully replicated DNA, indicating that inhibition is due to a reduction in the frequency of initiation and not the rate or extent of elongation. The observation that H1 dramatically reduces the number of replication foci in sperm nuclei supports this view. The establishment of replication competent DNA in egg extract requires the assembly of prereplication complexes (pre-RCs) on sperm chromatin. H1 reduces binding of the pre-RC proteins, XOrc2, XCdc6, and XMcm3, to chromatin. Replication competence can be restored in these nuclei, however, only under conditions that promote the loss of H1 from chromatin and licensing of the DNA. Thus, H1 inhibits replication in egg extract by preventing the assembly of pre-RCs on sperm chromatin, thereby reducing the frequency of initiation. These data raise the interesting possibility that H1 plays a role in regulating replication origin use during Xenopus development.
Adachi,
Identification of nuclear pre-replication centers poised for DNA synthesis in Xenopus egg extracts: immunolocalization study of replication protein A.
1992, Pubmed,
Xenbase
Adachi,
Identification of nuclear pre-replication centers poised for DNA synthesis in Xenopus egg extracts: immunolocalization study of replication protein A.
1992,
Pubmed
,
Xenbase
Adachi,
Study of the cell cycle-dependent assembly of the DNA pre-replication centres in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase
Bergman,
Chicken histone H5 inhibits transcription and replication when introduced into proliferating cells by microinjection.
1988,
Pubmed
Blow,
Nuclei act as independent and integrated units of replication in a Xenopus cell-free DNA replication system.
1987,
Pubmed
,
Xenbase
Blow,
Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor.
1993,
Pubmed
,
Xenbase
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Bouvet,
Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1.
1994,
Pubmed
,
Xenbase
Brown,
Differential effect of H1 variant overexpression on cell cycle progression and gene expression.
1996,
Pubmed
Callan,
Replication of DNA in the chromosomes of eukaryotes.
1972,
Pubmed
Carpenter,
Role for a Xenopus Orc2-related protein in controlling DNA replication.
1996,
Pubmed
,
Xenbase
Chipev,
Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo.
1992,
Pubmed
,
Xenbase
Chong,
Purification of an MCM-containing complex as a component of the DNA replication licensing system.
1995,
Pubmed
,
Xenbase
Coleman,
The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts.
1996,
Pubmed
,
Xenbase
Cox,
DNA replication occurs at discrete sites in pseudonuclei assembled from purified DNA in vitro.
1991,
Pubmed
,
Xenbase
Cozzarelli,
The mechanism of action of inhibitors of DNA synthesis.
1977,
Pubmed
Dasso,
Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: studies in Xenopus.
1990,
Pubmed
,
Xenbase
Dasso,
Nuclear assembly is independent of linker histones.
1994,
Pubmed
,
Xenbase
Dimitrov,
Remodeling sperm chromatin in Xenopus laevis egg extracts: the role of core histone phosphorylation and linker histone B4 in chromatin assembly.
1994,
Pubmed
,
Xenbase
Dimitrov,
Chromatin transitions during early Xenopus embryogenesis: changes in histone H4 acetylation and in linker histone type.
1993,
Pubmed
,
Xenbase
Dimitrov,
Remodeling somatic nuclei in Xenopus laevis egg extracts: molecular mechanisms for the selective release of histones H1 and H1(0) from chromatin and the acquisition of transcriptional competence.
1996,
Pubmed
,
Xenbase
Dworkin-Rastl,
The maternal histone H1 variant, H1M (B4 protein), is the predominant H1 histone in Xenopus pregastrula embryos.
1994,
Pubmed
,
Xenbase
Gurdon,
"Fertile" intestine nuclei.
1966,
Pubmed
Halmer,
Influence of histone H1 on the in vitro replication of DNA and chromatin.
1995,
Pubmed
Hock,
Absence of somatic histone H1 in oocytes and preblastula embryos of Xenopus laevis.
1993,
Pubmed
,
Xenbase
Hyrien,
Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos.
1993,
Pubmed
,
Xenbase
Hyrien,
Transition in specification of embryonic metazoan DNA replication origins.
1995,
Pubmed
,
Xenbase
Jackson,
Early events in DNA replication require cyclin E and are blocked by p21CIP1.
1995,
Pubmed
,
Xenbase
Kandolf,
The H1A histone variant is an in vivo repressor of oocyte-type 5S gene transcription in Xenopus laevis embryos.
1994,
Pubmed
,
Xenbase
Khochbin,
Developmentally regulated expression of linker-histone variants in vertebrates.
1994,
Pubmed
Kubota,
Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor.
1995,
Pubmed
,
Xenbase
Lawlis,
Chromosome architecture can dictate site-specific initiation of DNA replication in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Leno,
The nuclear membrane determines the timing of DNA replication in Xenopus egg extracts.
1991,
Pubmed
,
Xenbase
Leno,
Initiation of DNA replication in nuclei from quiescent cells requires permeabilization of the nuclear membrane.
1994,
Pubmed
,
Xenbase
Liang,
ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome.
1995,
Pubmed
Lu,
Histone H1 modulates DNA replication through multiple pathways in Xenopus egg extract.
1997,
Pubmed
,
Xenbase
Madine,
The nuclear envelope prevents reinitiation of replication by regulating the binding of MCM3 to chromatin in Xenopus egg extracts.
1995,
Pubmed
,
Xenbase
Madine,
MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells.
1995,
Pubmed
,
Xenbase
Mahbubani,
Cell cycle regulation of the replication licensing system: involvement of a Cdk-dependent inhibitor.
1997,
Pubmed
,
Xenbase
Mahbubani,
DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Micheli,
Chromosome length and DNA loop size during early embryonic development of Xenopus laevis.
1993,
Pubmed
,
Xenbase
Mills,
Replication occurs at discrete foci spaced throughout nuclei replicating in vitro.
1989,
Pubmed
,
Xenbase
Munshi,
Replication of nuclei from cycling and quiescent mammalian cells in 6-DMAP-treated Xenopus egg extract.
1998,
Pubmed
,
Xenbase
Méchali,
DNA synthesis in a cell-free system from Xenopus eggs: priming and elongation on single-stranded DNA in vitro.
1982,
Pubmed
,
Xenbase
Méchali,
Lack of specific sequence requirement for DNA replication in Xenopus eggs compared with high sequence specificity in yeast.
1984,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Nightingale,
Evidence for a shared structural role for HMG1 and linker histones B4 and H1 in organizing chromatin.
1996,
Pubmed
,
Xenbase
Romanowski,
The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin.
1996,
Pubmed
,
Xenbase
Romanowski,
Mechanisms restricting DNA replication to once per cell cycle: MCMS, pre-replicative complexes and kinases.
1996,
Pubmed
Romanowski,
Mechanisms restricting DNA replication to once per cell cycle: the role of Cdc6p and ORC.
1997,
Pubmed
Rowles,
Interaction between the origin recognition complex and the replication licensing system in Xenopus.
1996,
Pubmed
,
Xenbase
Shimamura,
Histone H1 represses transcription from minichromosomes assembled in vitro.
1989,
Pubmed
,
Xenbase
Steinbach,
Somatic linker histones cause loss of mesodermal competence in Xenopus.
1997,
Pubmed
,
Xenbase
Stillman,
Cell cycle control of DNA replication.
1996,
Pubmed
Sun,
Histone H5 in the control of DNA synthesis and cell proliferation.
1989,
Pubmed
Thömmes,
The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides.
1997,
Pubmed
,
Xenbase
Trieschmann,
Incorporation of chromosomal proteins HMG-14/HMG-17 into nascent nucleosomes induces an extended chromatin conformation and enhances the utilization of active transcription complexes.
1995,
Pubmed
,
Xenbase
Ura,
A positive role for nucleosome mobility in the transcriptional activity of chromatin templates: restriction by linker histones.
1995,
Pubmed
,
Xenbase
Ura,
Differential association of HMG1 and linker histones B4 and H1 with dinucleosomal DNA: structural transitions and transcriptional repression.
1996,
Pubmed
,
Xenbase
Walter,
Regulation of replicon size in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase
Wolffe,
Dominant and specific repression of Xenopus oocyte 5S RNA genes and satellite I DNA by histone H1.
1989,
Pubmed
,
Xenbase
Yan,
FFA-1, a protein that promotes the formation of replication centers within nuclei.
1995,
Pubmed
,
Xenbase
