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XB-ART-15224
Nat Biotechnol 1998 Mar 01;163:253-7. doi: 10.1038/nbt0398-253.
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Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus.

Fu Y , Wang Y , Evans SM .


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Expression of transgenes within a single generation by direct DNA injection into vertebrate embryos has been plagued by inefficient and nonuniform gene expression. We report a novel strategy for efficient and stable expression of transgenes driven by both ubiquitous and tissue-specific promoters by direct DNA injection into developing Xenopus laevis embryos. This strategy involves flanking expression cassettes of interest with inverted terminal repeat sequences (ITRs) from adeno-associated virus. Our results suggest that the ITR strategy may be generally applicable to other systems, such as zebra fish and embryonic stem cells, and may enable tissue-specific expression of transgenes in problematic contexts.

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References :
Sheets, Turning the frog into a princely model. 1998, Pubmed, Xenbase