Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
XMAP310: a Xenopus rescue-promoting factor localized to the mitotic spindle.
Andersen SS
,
Karsenti E
.
???displayArticle.abstract???
To understand the role of microtubule-associated proteins (MAPs) in the regulation of microtubule (MT) dynamics we have characterized MAPs prepared from Xenopus laevis eggs (Andersen, S.S.L., B. Buendia, J.E. Domínguez, A. Sawyer, and E. Karsenti. 1994. J. Cell Biol. 127:1289-1299). Here we report on the purification and characterization of a 310-kD MAP (XMAP310) that localizes to the nucleus in interphase and to mitotic spindle MTs in mitosis. XMAP310 is present in eggs, oocytes, a Xenopus tissue culture cell line, testis, and brain. We have purified XMAP310 to homogeneity from egg extracts. The purified protein cross-links pure MTs. Analysis of the effect of this protein on MT dynamics by time-lapse video microscopy has shown that it increases the rescue frequency 5-10-fold and decreases the shrinkage rate twofold. It has no effect on the growth rate or the catastrophe frequency. Microsequencing data suggest that XMAP230 and XMAP310 are novel MAPs. Although the three Xenopus MAPs characterized so far, XMAP215 (Vasquez, R.J., D.L. Gard, and L. Cassimeris. 1994. J. Cell Biol. 127:985-993), XMAP230, and XMAP310 are localized to the mitotic spindle, they have distinct effects on MT dynamics. While XMAP215 promotes rapid MT growth, XMAP230 decreases the catastrophe frequency and XMAP310 increases the rescue frequency. This may have important implications for the regulation of MT dynamics during spindle morphogenesis and chromosome segregation.
Figure 1. XMAP310 localization in XL177 cells (Miller and Daniel, 1977) during the cell cycle. XL177 cells were stained with: Hoechst (a, d, g), a rabbit anti-tubulin pAb (b, e, h, j, l), and the Q4 mAb (c, f, i, k, m). Interphase (a–c), metaphase/early anaphase after pr-extraction with 0.5% TX-100 (d–f), late anaphase (g–i), late telophase without preextraction (j and k), late telophase with preextraction (l and m). Bar: (a–k) 10 μm; (l and m) 12.5 μm.
Figure 2. (Top) XMAP310 localization in the mitotic spindle: MTs stained with a rabbit anti-tublin pAb in the rhodamine channel (Tubulin), XMAP310 in the fluorescein channel (XMAP310) and in overlay (Overlay). (Bottom) XMAP230 and XMAP310 colocalize (Overlay) to the central region of the mitotic spindle in XL177 cells. Images were obtained by confocal microscopy. Bar, 2.5 μm.
Figure 4. Electron micrographs showing cross-linked MTs in bundles formed in the presence of XMAP310. After spontaneous assembly in the presence XMAP310, MTs were spotted onto carbon grids and visualized by negative stain and electron microscopy. (a) A long bundle consisting of three cross-linked MTs (b) magnification of the arrow-outlined area in a, note the uniform short spacing of the MTs. Bars: (a) 200 nm; (b) 60 nm.
Figure 5. MT dynamics visualized by video microscopy. MTs are nucleated by a centrosome (center of the MT asters). (a) MTs polymerized in the absence of XMAP310. (b–d) MTs polymerized with XMAP310. The MT indicated with an arrow experienced a catastrophe shortly after the frame shown in b, and shrank to the length shown in c where it was rescued and continued to grow, as shown in d. Time since transfer to 37°C is indicated by xx:xx:xx, which represents hours:minutes:seconds, respectively. Bar, 10 μm.
Figure 6. Model for how XMAP310 may promote rescues and MT cross-linking. (Top) Plane-projection of a depolymerizing (shrinking) MT in the absence (Control) or presence of XMAP310 (+XMAP310), with protofilaments (lines) peeling off the MT by out-wards curvature (arrows). Note the more blunt MT end in the presence of XMAP310 which could be promoted if cross-linking of protofilaments by XMAP310 prevents the outwards curvature of protofilaments. (Bottom) Magnifications of the boxed areas, showing part of the MTs at two different time points, t1 and t2. In the Control, the MT is shrinking both at t1 and t2, by loss of GDP-tubulin (arrows). In the presence of XMAP310 the MT is shrinking at t1. At t2 the MT has been rescued. A depolymerizing MT (t1) will meet resistance towards depolymerization due to the cross-linking of protofilaments/MTs, but XMAP310 does not dramatically prevent the loss of the tubulin dimers from the depolymerizing ends (little effect on vs). Eventually depolymerization of the MT is blocked long enough to maintain the depolymerizing MT-GDP-lattice in a straight conformation allowing new GTP-tubulin subunits to be added to the end, and the MT has been rescued (arrows indicate the direction of the flux of tubulin subunits).
Andersen,
Effect on microtubule dynamics of XMAP230, a microtubule-associated protein present in Xenopus laevis eggs and dividing cells.
1994, Pubmed,
Xenbase
Andersen,
Effect on microtubule dynamics of XMAP230, a microtubule-associated protein present in Xenopus laevis eggs and dividing cells.
1994,
Pubmed
,
Xenbase
Andersen,
Mitotic chromatin regulates phosphorylation of Stathmin/Op18.
1997,
Pubmed
,
Xenbase
Belmont,
Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.
1996,
Pubmed
,
Xenbase
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase
Bonifacino,
A widely distributed nuclear protein immunologically related to the microtubule-associated protein MAP1 is associated with the mitotic spindle.
1985,
Pubmed
Bornens,
Structural and chemical characterization of isolated centrosomes.
1987,
Pubmed
,
Xenbase
Bulinski,
Purification and characterization of ensconsin, a novel microtubule stabilizing protein.
1994,
Pubmed
Charrasse,
Characterization of the cDNA and pattern of expression of a new gene over-expressed in human hepatomas and colonic tumors.
1995,
Pubmed
Chen,
Projection domains of MAP2 and tau determine spacings between microtubules in dendrites and axons.
1992,
Pubmed
Chrétien,
Structure of growing microtubule ends: two-dimensional sheets close into tubes at variable rates.
1995,
Pubmed
Cleveland,
NuMA: a protein involved in nuclear structure, spindle assembly, and nuclear re-formation.
1995,
Pubmed
Collins,
Reversible assembly purification of taxol-treated microtubules.
1991,
Pubmed
Compton,
Identification of novel centromere/kinetochore-associated proteins using monoclonal antibodies generated against human mitotic chromosome scaffolds.
1991,
Pubmed
Cormier,
Molecular cloning of Xenopus elongation factor 1 gamma, major M-phase promoting factor substrate.
1991,
Pubmed
,
Xenbase
Dogterom,
Influence of M-phase chromatin on the anisotropy of microtubule asters.
1996,
Pubmed
,
Xenbase
Drechsel,
Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau.
1992,
Pubmed
Evans,
Influence of the centrosome on the structure of nucleated microtubules.
1985,
Pubmed
Feick,
Immunolocalization and molecular properties of a high molecular weight microtubule-bundling protein (syncolin) from chicken erythrocytes.
1991,
Pubmed
Gard,
A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end.
1987,
Pubmed
,
Xenbase
Heald,
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Hershey,
Translational control in mammalian cells.
1991,
Pubmed
Hirokawa,
Microtubule organization and dynamics dependent on microtubule-associated proteins.
1994,
Pubmed
Hirokawa,
Tau proteins: the molecular structure and mode of binding on microtubules.
1988,
Pubmed
Holy,
Dynamic instability of microtubules as an efficient way to search in space.
1994,
Pubmed
Hyman,
Preparation of modified tubulins.
1991,
Pubmed
Hyman,
Morphogenetic properties of microtubules and mitotic spindle assembly.
1996,
Pubmed
Inoué,
Force generation by microtubule assembly/disassembly in mitosis and related movements.
1995,
Pubmed
Itoh,
Microtubule-stabilizing activity of microtubule-associated proteins (MAPs) is due to increase in frequency of rescue in dynamic instability: shortening length decreases with binding of MAPs onto microtubules.
1994,
Pubmed
Izant,
A microtubule-associated protein in the mitotic spindle and the interphase nucleus.
1982,
Pubmed
Jensen,
Delayed extraction improves specificity in database searches by matrix-assisted laser desorption/ionization peptide maps.
1996,
Pubmed
Karsenti,
Respective roles of centrosomes and chromatin in the conversion of microtubule arrays from interphase to metaphase.
1984,
Pubmed
,
Xenbase
Kirschner,
Beyond self-assembly: from microtubules to morphogenesis.
1986,
Pubmed
Mandelkow,
Microtubule dynamics and microtubule caps: a time-resolved cryo-electron microscopy study.
1991,
Pubmed
Mandelkow,
Microtubules and microtubule-associated proteins.
1995,
Pubmed
Mangan,
A muscle-specific variant of microtubule-associated protein 4 (MAP4) is required in myogenesis.
1996,
Pubmed
Masson,
Binding of E-MAP-115 to microtubules is regulated by cell cycle-dependent phosphorylation.
1995,
Pubmed
Mastronarde,
Interpolar spindle microtubules in PTK cells.
1993,
Pubmed
Matus,
Microtubule-associated proteins.
1990,
Pubmed
McNally,
Katanin, the microtubule-severing ATPase, is concentrated at centrosomes.
1996,
Pubmed
Merdes,
A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly.
1996,
Pubmed
,
Xenbase
Miller,
Comparison of in vivo and in vitro ribosomal RNA synthesis in nucleolar mutants of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Nguyen,
Overexpression of full- or partial-length MAP4 stabilizes microtubules and alters cell growth.
1997,
Pubmed
Olmsted,
Microtubule-associated proteins.
1986,
Pubmed
Olmsted,
Cell cycle-dependent changes in the dynamics of MAP 2 and MAP 4 in cultured cells.
1989,
Pubmed
Ookata,
Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics.
1995,
Pubmed
Pepper,
Studies of a microtubule-associated protein using a monoclonal antibody elicited against mammalian mitotic spindles.
1984,
Pubmed
Pereira,
Genetic analysis of a Drosophila microtubule-associated protein.
1992,
Pubmed
Pryer,
Brain microtubule-associated proteins modulate microtubule dynamic instability in vitro. Real-time observations using video microscopy.
1992,
Pubmed
Reinsch,
Orientation of spindle axis and distribution of plasma membrane proteins during cell division in polarized MDCKII cells.
1994,
Pubmed
Sato-Yoshitake,
Microtubule-associated protein 1B: molecular structure, localization, and phosphorylation-dependent expression in developing neurons.
1989,
Pubmed
Saunders,
The Drosophila gene abnormal spindle encodes a novel microtubule-associated protein that associates with the polar regions of the mitotic spindle.
1997,
Pubmed
Sawin,
Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules.
1994,
Pubmed
,
Xenbase
Saxton,
Tubulin dynamics in cultured mammalian cells.
1984,
Pubmed
Shiina,
Regulation of a major microtubule-associated protein by MPF and MAP kinase.
1992,
Pubmed
,
Xenbase
Shiina,
A novel homo-oligomeric protein responsible for an MPF-dependent microtubule-severing activity.
1992,
Pubmed
,
Xenbase
Simon,
The structure of microtubule ends during the elongation and shortening phases of dynamic instability examined by negative-stain electron microscopy.
1990,
Pubmed
Sobel,
Stathmin: a relay phosphoprotein for multiple signal transduction?
1991,
Pubmed
Toso,
Kinetic stabilization of microtubule dynamic instability in vitro by vinblastine.
1993,
Pubmed
Tournebize,
Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis.
1997,
Pubmed
,
Xenbase
Trinczek,
Domains of tau protein, differential phosphorylation, and dynamic instability of microtubules.
1995,
Pubmed
Vale,
Severing of stable microtubules by a mitotically activated protein in Xenopus egg extracts.
1991,
Pubmed
,
Xenbase
Vasquez,
XMAP from Xenopus eggs promotes rapid plus end assembly of microtubules and rapid microtubule polymer turnover.
1994,
Pubmed
,
Xenbase
Verde,
Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Verde,
Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs.
1990,
Pubmed
,
Xenbase
Walker,
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
1988,
Pubmed
Wang,
Removal of MAP4 from microtubules in vivo produces no observable phenotype at the cellular level.
1996,
Pubmed
Waters,
Pathways of spindle assembly.
1997,
Pubmed
Wiche,
Molecular structure and function of microtubule-associated proteins.
1991,
Pubmed
Wilhelm,
Purification of recombinant cyclin B1/cdc2 kinase from Xenopus egg extracts.
1997,
Pubmed
,
Xenbase
Zhai,
Microtubule dynamics at the G2/M transition: abrupt breakdown of cytoplasmic microtubules at nuclear envelope breakdown and implications for spindle morphogenesis.
1996,
Pubmed
Zieve,
Proteins specifically associated with the microtubules of the mammalian mitotic spindle.
1982,
Pubmed
