Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Cell
1997 Oct 01;810:2017-38. doi: 10.1091/mbc.8.10.2017.
Show Gene links
Show Anatomy links
Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly.
Grandi P
,
Dang T
,
Pané N
,
Shevchenko A
,
Mann M
,
Forbes D
,
Hurt E
.
???displayArticle.abstract???
Yeast and vertebrate nuclear pores display significant morphological similarity by electron microscopy, but sequence similarity between the respective proteins has been more difficult to observe. Herein we have identified a vertebrate nucleoporin, Nup93, in both human and Xenopus that has proved to be an evolutionarily related homologue of the yeast nucleoporin Nic96p. Polyclonal antiserum to human Nup93 detects corresponding proteins in human, rat, and Xenopus cells. Immunofluorescence and immunoelectron microscopy localize vertebrate Nup93 at the nuclear basket and at or near the nuclear entry to the gated channel of the pore. Immunoprecipitation from both mammalian and Xenopus cell extracts indicates that a small fraction of Nup93 physically interacts with the nucleoporin p62, just as yeast Nic96p interacts with the yeast p62 homologue. However, a large fraction of vertebrate Nup93 is extracted from pores and is also present in Xenopus egg extracts in complex with a newly discovered 205-kDa protein. Mass spectrometric sequencing of the human 205-kDa protein reveals that this protein is encoded by an open reading frame, KIAAO225, present in the human database. The putative human nucleoporin of 205 kDa has related sequence homologues in Caenorhabditis elegans and Saccharomyces cerevisiae. The analyze the role of the Nup93 complex in the pore, nuclei were assembled that lack the Nup93 complex after immunodepletion of a Xenopus nuclear reconstitution extract. The Nup93-complex-depleted nuclei are clearly defective for correct nuclear pore assembly. From these experiments, we conclude that the vertebrate and yeast pore have significant homology in their functionally important cores and that, with the identification of Nup93 and the 205-kDa protein, we have extended the knowledge of the nearest-neighbor interactions of this core in both yeast and vertebrates.
Aitchison,
Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
1995, Pubmed
Aitchison,
Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
1995,
Pubmed
Akey,
Architecture of the Xenopus nuclear pore complex revealed by three-dimensional cryo-electron microscopy.
1993,
Pubmed
,
Xenbase
Akey,
Interactions and structure of the nuclear pore complex revealed by cryo-electron microscopy.
1989,
Pubmed
,
Xenbase
Allende,
Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development.
1996,
Pubmed
,
Xenbase
Becker,
RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae.
1995,
Pubmed
Blobel,
Nuclei from rat liver: isolation method that combines purity with high yield.
1966,
Pubmed
Buss,
Macromolecular interactions in the nucleoporin p62 complex of rat nuclear pores: binding of nucleoporin p54 to the rod domain of p62.
1995,
Pubmed
Byrd,
Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex.
1994,
Pubmed
Carmo-Fonseca,
Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization.
1991,
Pubmed
Chi,
Sequence and characterization of cytoplasmic nuclear protein import factor p97.
1995,
Pubmed
Clarkson,
Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p.
1996,
Pubmed
Corbett,
The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo.
1996,
Pubmed
Cordes,
Intranuclear filaments containing a nuclear pore complex protein.
1993,
Pubmed
,
Xenbase
Cordes,
Identification of protein p270/Tpr as a constitutive component of the nuclear pore complex-attached intranuclear filaments.
1997,
Pubmed
,
Xenbase
Dabauvalle,
Identification of a soluble precursor complex essential for nuclear pore assembly in vitro.
1990,
Pubmed
,
Xenbase
Davis,
The nuclear pore complex.
1995,
Pubmed
,
Xenbase
Davis,
Identification and characterization of a nuclear pore complex protein.
1986,
Pubmed
Doye,
Genetic approaches to nuclear pore structure and function.
1995,
Pubmed
Doye,
From nucleoporins to nuclear pore complexes.
1997,
Pubmed
Enenkel,
Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes.
1995,
Pubmed
Fabre,
Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif.
1994,
Pubmed
Finlay,
Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
1987,
Pubmed
,
Xenbase
Finlay,
Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored.
1990,
Pubmed
,
Xenbase
Finlay,
A complex of nuclear pore proteins required for pore function.
1991,
Pubmed
Fornerod,
The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.
1997,
Pubmed
Gerace,
Organization and modulation of nuclear lamina structure.
1984,
Pubmed
Goldberg,
Structural and functional organization of the nuclear envelope.
1995,
Pubmed
,
Xenbase
Goldberg,
High resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores.
1992,
Pubmed
,
Xenbase
Gorsch,
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
1995,
Pubmed
Grandi,
A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p.
1995,
Pubmed
Grandi,
Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.
1995,
Pubmed
Grandi,
Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96.
1993,
Pubmed
Greber,
A major glycoprotein of the nuclear pore complex is a membrane-spanning polypeptide with a large lumenal domain and a small cytoplasmic tail.
1990,
Pubmed
Grote,
Mapping of nucleoporins to the center of the nuclear pore complex by post-embedding immunogold electron microscopy.
1995,
Pubmed
Guan,
Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.
1995,
Pubmed
Görlich,
Isolation of a protein that is essential for the first step of nuclear protein import.
1994,
Pubmed
,
Xenbase
Görlich,
Importin provides a link between nuclear protein import and U snRNA export.
1996,
Pubmed
,
Xenbase
Hallberg,
An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region.
1993,
Pubmed
Heath,
Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene.
1995,
Pubmed
Hu,
Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins.
1996,
Pubmed
Jarnik,
Toward a more complete 3-D structure of the nuclear pore complex.
1991,
Pubmed
,
Xenbase
Kenna,
Yeast N1e3p/Nup170p is required for normal stoichiometry of FG nucleoporins within the nuclear pore complex.
1996,
Pubmed
Kita,
Purification and characterization of a nuclear pore glycoprotein complex containing p62.
1993,
Pubmed
Kraemer,
The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm.
1994,
Pubmed
Li,
Mutation or deletion of the Saccharomyces cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A)+ RNA and constitutive clustering of nuclear pore complexes.
1995,
Pubmed
Loeb,
The yeast nuclear import receptor is required for mitosis.
1995,
Pubmed
MILLONIG,
A modified procedure for lead staining of thin sections.
1961,
Pubmed
Macaulay,
Differential mitotic phosphorylation of proteins of the nuclear pore complex.
1995,
Pubmed
,
Xenbase
Macaulay,
Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTP gamma S, and BAPTA.
1996,
Pubmed
,
Xenbase
Mahajan,
A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2.
1997,
Pubmed
Mann,
Electrospray mass spectrometry for protein characterization.
1995,
Pubmed
Mann,
Error-tolerant identification of peptides in sequence databases by peptide sequence tags.
1994,
Pubmed
Moore,
The GTP-binding protein Ran/TC4 is required for protein import into the nucleus.
1993,
Pubmed
,
Xenbase
Nakamura,
Fusion of the nucleoporin gene NUP98 to HOXA9 by the chromosome translocation t(7;11)(p15;p15) in human myeloid leukaemia.
1996,
Pubmed
Nehrbass,
Analysis of nucleo-cytoplasmic transport in a thermosensitive mutant of nuclear pore protein NSP1.
1993,
Pubmed
Nehrbass,
The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex.
1996,
Pubmed
Nehrbass,
NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain.
1990,
Pubmed
Panté,
Interactions and three-dimensional localization of a group of nuclear pore complex proteins.
1994,
Pubmed
,
Xenbase
Panté,
Exploring nuclear pore complex structure and function in molecular detail.
1995,
Pubmed
,
Xenbase
Powers,
Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.
1995,
Pubmed
,
Xenbase
Radu,
Nup107 is a novel nuclear pore complex protein that contains a leucine zipper.
1994,
Pubmed
Radu,
The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex.
1995,
Pubmed
Radu,
Nup155 is a novel nuclear pore complex protein that contains neither repetitive sequence motifs nor reacts with WGA.
1993,
Pubmed
Ren,
Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing.
1995,
Pubmed
Rout,
Isolation of the yeast nuclear pore complex.
1993,
Pubmed
Schlaich,
Analysis of nucleocytoplasmic transport and nuclear envelope structure in yeast disrupted for the gene encoding the nuclear pore protein Nup1p.
1995,
Pubmed
Schlaich,
In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p.
1997,
Pubmed
Shevchenko,
Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels.
1996,
Pubmed
Shevchenko,
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.
1996,
Pubmed
Shulga,
In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation.
1996,
Pubmed
Simos,
Nucleocytoplasmic transport: factors and mechanisms.
1995,
Pubmed
,
Xenbase
Siniossoglou,
A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores.
1996,
Pubmed
Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed
Sukegawa,
A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm.
1993,
Pubmed
Unwin,
A large particle associated with the perimeter of the nuclear pore complex.
1982,
Pubmed
,
Xenbase
Wente,
A new family of yeast nuclear pore complex proteins.
1992,
Pubmed
Wente,
A temperature-sensitive NUP116 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic.
1993,
Pubmed
Wilm,
Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry.
1996,
Pubmed
Wilm,
Analytical properties of the nanoelectrospray ion source.
1996,
Pubmed
Wozniak,
The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope.
1992,
Pubmed
Wu,
Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region.
1995,
Pubmed
Yano,
Yeast Srp1p has homology to armadillo/plakoglobin/beta-catenin and participates in apparently multiple nuclear functions including the maintenance of the nucleolar structure.
1994,
Pubmed
Yokoyama,
A giant nucleopore protein that binds Ran/TC4.
1995,
Pubmed
Zabel,
Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p.
1996,
Pubmed
Zimowska,
A Drosophila Tpr protein homolog is localized both in the extrachromosomal channel network and to nuclear pore complexes.
1997,
Pubmed
