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Mol Cell Biol
1997 Oct 01;1710:5833-42. doi: 10.1128/MCB.17.10.5833.
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Nucleosome binding by the polymerase I transactivator upstream binding factor displaces linker histone H1.
Kermekchiev M
,
Workman JL
,
Pikaard CS
.
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Upstream binding factor (UBF) is a vertebrate RNA polymerase I transcription factor that can bend and wrap DNA. To investigate UBF's likely role as an architectural protein of rRNA genes organized in chromatin, we tested UBF's ability to bind rRNA gene enhancers assembled into nucleosome cores (DNA plus core histones) and nucleosomes (DNA plus core histones plus histone H1). UBF bound with low affinity to nucleosome cores formed with enhancer DNA probes of 162 bp. However, on nucleosome cores which contained approximately 60 bp of additional linker DNA, UBF bound with high affinity similar to its binding to naked DNA, forming a ternary DNA-core histone-UBF complex. UBF could be stripped from ternary complexes with competitor DNA to liberate nucleosome cores, rather than free DNA, suggesting that UBF binding to nucleosome cores does not displace the core histones H2A, H2B, H3, and H4. DNase I, micrococcal nuclease, and exonuclease III footprinting suggests that UBF and histone H1 interact with DNA on both sides flanking the histone octamer. Footprinting shows that UBF outcompetes histone H1 for binding to a nucleosome core and will displace, if not dissociate, H1 from its binding site on a preassembled nucleosome. These data suggest that UBF may act to prevent or reverse the assembly of transcriptionally inactive chromatin structures catalyzed by linker histone binding.
Bazett-Jones,
Short-range DNA looping by the Xenopus HMG-box transcription factor, xUBF.
1994, Pubmed,
Xenbase
Bazett-Jones,
Short-range DNA looping by the Xenopus HMG-box transcription factor, xUBF.
1994,
Pubmed
,
Xenbase
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Boseley,
Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Buratowski,
Five intermediate complexes in transcription initiation by RNA polymerase II.
1989,
Pubmed
Busby,
Spacer sequences regulate transcription of ribosomal gene plasmids injected into Xenopus embryos.
1983,
Pubmed
,
Xenbase
Clark,
Effect of positive supercoiling on DNA compaction by nucleosome cores.
1993,
Pubmed
Conconi,
Two different chromatin structures coexist in ribosomal RNA genes throughout the cell cycle.
1989,
Pubmed
Copenhaver,
The RNA polymerase I transcription factor UBF is a sequence-tolerant HMG-box protein that can recognize structured nucleic acids.
1994,
Pubmed
,
Xenbase
Dammann,
Transcription in the yeast rRNA gene locus: distribution of the active gene copies and chromatin structure of their flanking regulatory sequences.
1995,
Pubmed
Dammann,
Chromatin structures and transcription of rDNA in yeast Saccharomyces cerevisiae.
1993,
Pubmed
Dimitrov,
Chromatin transitions during early Xenopus embryogenesis: changes in histone H4 acetylation and in linker histone type.
1993,
Pubmed
,
Xenbase
Ding,
Stimulation of RNA polymerase II elongation by chromosomal protein HMG-14.
1994,
Pubmed
Elgin,
Chromatin structure and gene activity.
1990,
Pubmed
Felsenfeld,
Chromatin as an essential part of the transcriptional mechanism.
1992,
Pubmed
Finch,
Solenoidal model for superstructure in chromatin.
1976,
Pubmed
Grossbach,
Selective distribution of histone H1 variants and high mobility group proteins in chromosomes.
1995,
Pubmed
Hu,
xUBF, an RNA polymerase I transcription factor, binds crossover DNA with low sequence specificity.
1994,
Pubmed
,
Xenbase
Jantzen,
Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins.
1990,
Pubmed
Juan,
H1-mediated repression of transcription factor binding to a stably positioned nucleosome.
1997,
Pubmed
Khochbin,
Developmentally regulated expression of linker-histone variants in vertebrates.
1994,
Pubmed
Kornberg,
Chromatin structure: a repeating unit of histones and DNA.
1974,
Pubmed
Kornberg,
Interplay between chromatin structure and transcription.
1995,
Pubmed
Kuhn,
Dual role of the nucleolar transcription factor UBF: trans-activator and antirepressor.
1992,
Pubmed
Kuhn,
Functional differences between the two splice variants of the nucleolar transcription factor UBF: the second HMG box determines specificity of DNA binding and transcriptional activity.
1994,
Pubmed
Labhart,
Enhancer-like properties of the 60/81 bp elements in the ribosomal gene spacer of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Laudet,
Ancestry and diversity of the HMG box superfamily.
1993,
Pubmed
Laybourn,
Role of nucleosomal cores and histone H1 in regulation of transcription by RNA polymerase II.
1991,
Pubmed
Leblanc,
Recognition of the Xenopus ribosomal core promoter by the transcription factor xUBF involves multiple HMG box domains and leads to an xUBF interdomain interaction.
1993,
Pubmed
,
Xenbase
Lorch,
Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
1987,
Pubmed
McKnight,
Ultrastructural patterns of RNA synthesis during early embryogenesis of Drosophila melanogaster.
1976,
Pubmed
McStay,
The Xenopus RNA polymerase I transcription factor, UBF, has a role in transcriptional enhancement distinct from that at the promoter.
1997,
Pubmed
,
Xenbase
McStay,
xUBF contains a novel dimerization domain essential for RNA polymerase I transcription.
1991,
Pubmed
,
Xenbase
Moss,
Promotion and regulation of ribosomal transcription in eukaryotes by RNA polymerase I.
1995,
Pubmed
Moss,
A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis.
,
Pubmed
,
Xenbase
Mougey,
Virtually the entire Xenopus laevis rDNA multikilobase intergenic spacer serves to stimulate polymerase I transcription.
1996,
Pubmed
,
Xenbase
Neil,
Structure of recombinant rat UBF by electron image analysis and homology modelling.
1996,
Pubmed
Neubauer,
Analysis of nucleosome positioning by in vitro reconstitution.
1989,
Pubmed
Nightingale,
Evidence for a shared structural role for HMG1 and linker histones B4 and H1 in organizing chromatin.
1996,
Pubmed
,
Xenbase
Noll,
Action of micrococcal nuclease on chromatin and the location of histone H1.
1977,
Pubmed
Olins,
Spheroid chromatin units (v bodies).
1974,
Pubmed
Owen-Hughes,
Experimental analysis of chromatin function in transcription control.
1994,
Pubmed
Paranjape,
HMG17 is a chromatin-specific transcriptional coactivator that increases the efficiency of transcription initiation.
1995,
Pubmed
Paranjape,
Role of chromatin structure in the regulation of transcription by RNA polymerase II.
1994,
Pubmed
Pennings,
Linker histones H1 and H5 prevent the mobility of positioned nucleosomes.
1994,
Pubmed
Pikaard,
Sequence elements essential for function of the Xenopus laevis ribosomal DNA enhancers.
1988,
Pubmed
,
Xenbase
Pikaard,
The Xenopus ribosomal gene enhancers bind an essential polymerase I transcription factor, xUBF.
1989,
Pubmed
,
Xenbase
Pikaard,
Enhancers for RNA polymerase I in mouse ribosomal DNA.
1990,
Pubmed
,
Xenbase
Postnikov,
Distribution of high mobility group proteins 1/2, E and 14/17 and linker histones H1 and H5 on transcribed and non-transcribed regions of chicken erythrocyte chromatin.
1991,
Pubmed
Pruss,
Nucleosomal anatomy--where are the histones?
1995,
Pubmed
Putnam,
Cooperative binding of the Xenopus RNA polymerase I transcription factor xUBF to repetitive ribosomal gene enhancers.
1992,
Pubmed
,
Xenbase
Putnam,
The RNA polymerase I transactivator upstream binding factor requires its dimerization domain and high-mobility-group (HMG) box 1 to bend, wrap, and positively supercoil enhancer DNA.
1994,
Pubmed
,
Xenbase
Quinn,
DNA-binding properties of the yeast SWI/SNF complex.
1996,
Pubmed
Rattner,
Higher order structure in metaphase chromosomes. I. The 250 A fiber.
1978,
Pubmed
Rattner,
Higher order structure in metaphase chromosomes. II. The relationship between the 250 A fiber, superbeads and beads-on-a-string.
1978,
Pubmed
Reeder,
Enhancers and ribosomal gene spacers.
1984,
Pubmed
,
Xenbase
Rhodes,
Assembly of nucleosomes and chromatin in vitro.
1989,
Pubmed
,
Xenbase
Simpson,
Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones.
1978,
Pubmed
Studitsky,
Overcoming a nucleosomal barrier to transcription.
1995,
Pubmed
Studitsky,
A histone octamer can step around a transcribing polymerase without leaving the template.
1994,
Pubmed
Thoma,
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
1979,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Treiber,
Cisplatin-DNA adducts are molecular decoys for the ribosomal RNA transcription factor hUBF (human upstream binding factor).
1994,
Pubmed
Ura,
A positive role for nucleosome mobility in the transcriptional activity of chromatin templates: restriction by linker histones.
1995,
Pubmed
,
Xenbase
Ura,
Reconstruction of transcriptionally active and silent chromatin.
1996,
Pubmed
,
Xenbase
Utley,
In vitro analysis of transcription factor binding to nucleosomes and nucleosome disruption/displacement.
1996,
Pubmed
Varga-Weisz,
Competition between linker histones and HMG1 for binding to four-way junction DNA: implications for transcription.
1994,
Pubmed
Vettese-Dadey,
Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro.
1996,
Pubmed
Weintraub,
Histone-H1-dependent chromatin superstructures and the suppression of gene activity.
1984,
Pubmed
Woodcock,
Chromatin organization re-viewed.
1995,
Pubmed
Zlatanova,
The linker histones and chromatin structure: new twists.
1996,
Pubmed
