XB-ART-15994EMBO J September 15, 1997; 16 (18): 5537-49.
Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis.
Assembly of a mitotic spindle requires the accurate regulation of microtubule dynamics which is accomplished, at least in part, by phosphorylation-dephosphorylation reactions. Here we have investigated the role of serine-threonine phosphatases in the control of microtubule dynamics using specific inhibitors in Xenopus egg extracts. Type 2A phosphatases are required to maintain the short steady-state length of microtubules in mitosis by regulating the level of microtubule catastrophes, in part by controlling the the microtubule-destabilizing activity and phosphorylation of Op18/stathmin. Type 1 phosphatases are only required for control of microtubule dynamics during the transitions into and out of mitosis. Thus, although both type 2A and type 1 phosphatases are involved in the regulation of microtubule dynamics, they have distinct, non-overlapping roles.
PubMed ID: 9312013
PMC ID: PMC1170186
Article link: EMBO J
Genes referenced: npy4r ptpa stmn1
References [+] :
Armstrong, Drosophila PPY, a novel male specific protein serine/threonine phosphatase localised in somatic cells of the testis. 1996, Pubmed