XB-ART-1621Dev Dyn September 1, 2005; 234 (1): 151-8.
Strategies to reduce variation in Xenopus regeneration studies.
In this study, we present strategies for experimental design that minimize variation in Xenopus hindlimb regeneration results. We have standardized our laboratory culture conditions for older stage Xenopus tadpoles. We have established a normal tadpole growth curve for our laboratory and characterized normal tadpole behaviors in an effort to eliminate abnormal tadpoles from our experiments. We have used large sample sizes and statistical analysis to establish normal regeneration performances for seven amputation planes in stages 55-57 tadpole hindlimbs. We have demonstrated that regeneration performance of abnormal tadpoles is significantly different than that of normal tadpoles. We have examined the kinetics of ossification in developing Xenopus hindlimbs, and have found that increasing ossification rates and rates of regeneration decline are inversely correlated.
PubMed ID: 16032711
Article link: Dev Dyn
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|Figure 2. Xenopus hindlimb amputation planes and regeneration scoring system. A: Amputations were performed in seven different amputation planes for tadpole hindlimbs in stages 54–59: femur (F), knee (K), tibia-fibula (TF), ankle joint (AJ), tarsus (T), tarsal-metatarsal joint (TMTJ), and metatarsals (MT). B: Using joints as boundaries, the skeletal element scoring system assigns 10 points for regeneration of the skeletal elements of each limb segment. Raw scores are computed by counting the regenerated skeletal elements for each segment. An index (percent) of regeneration is computed by dividing the raw score by the total possible score. The scores can then be compared within a stage, or between stages, and can all be graphed on a scale of 0–100% regeneration.Download figure to PowerPoint|