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sn-1,2-Diacylglycerol (DAG) mass and translocation of protein kinase C alpha and beta to a membrane fraction increased approximately 7 min after insemination of Xenopus laevis eggs. The DAG mass increase of 48 pmol (from 62 to 110 pmol/cell) was greater than that for inositol 1,4,5-trisphosphate (IP3; an increase of approximately 170 fmol or approximately 280-fold smaller than the DAG increase), and DAG peaks approximately 5 min after IP3. Choline mass (a measure of phosphatidyl choline-specific phospholipase D) also peaked before DAG and the choline increase (134 pmol/cell) was greater than that of DAG. There was no detectable change in phosphocholine mass (a measure of phosphatidylcholine-specific phospholipase C). During first cleavage, DAG decreased, PKC translocation was low, and choline increased and peaked (whereas published work shows an increase in IP3 mass). Artificial elevation of intracellular calcium ([Ca2+]i) increased DAG levels but prevention of the [Ca2+]i increase after fertilization did not block DAG production. Thus, sperm stimulate production of DAG and choline through [Ca2+]i-independent and [Ca2+]i-dependent paths.
Alonso,
Changes in triacylglycerol, diacylglycerol and free fatty acids after fertilization in developing toad embryos.
1986, Pubmed
Alonso,
Changes in triacylglycerol, diacylglycerol and free fatty acids after fertilization in developing toad embryos.
1986,
Pubmed
Arnold,
Effects of insulin and phorbol esters on MARCKS (myristoylated alanine-rich C-kinase substrate) phosphorylation (and other parameters of protein kinase C activation) in rat adipocytes, rat soleus muscle and BC3H-1 myocytes.
1993,
Pubmed
Bazzi,
Properties of the protein kinase C-phorbol ester interaction.
1989,
Pubmed
Bement,
Signal transduction by calcium and protein kinase C during egg activation.
1992,
Pubmed
Bement,
Activators of protein kinase C trigger cortical granule exocytosis, cortical contraction, and cleavage furrow formation in Xenopus laevis oocytes and eggs.
1989,
Pubmed
,
Xenbase
Bement,
Parallel pathways of cell cycle control during Xenopus egg activation.
1991,
Pubmed
,
Xenbase
Bement,
Protein kinase C acts downstream of calcium at entry into the first mitotic interphase of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Bement,
Analysis of inducible contractile rings suggests a role for protein kinase C in embryonic cytokinesis and wound healing.
1991,
Pubmed
,
Xenbase
Busa,
An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis.
1985,
Pubmed
,
Xenbase
Busa,
Activation of frog (Xenopus laevis) eggs by inositol trisphosphate. I. Characterization of Ca2+ release from intracellular stores.
1985,
Pubmed
,
Xenbase
Chen,
Molecular cloning and sequence analysis of two distinct types of Xenopus laevis protein kinase C.
,
Pubmed
,
Xenbase
Ciapa,
Polyphosphoinositide metabolism during the fertilization wave in sea urchin eggs.
1992,
Pubmed
Ciapa,
Cell-cycle calcium transients driven by cyclic changes in inositol trisphosphate levels.
1994,
Pubmed
Ciapa,
Two phases of inositol polyphosphate and diacylglycerol production at fertilisation.
1986,
Pubmed
Ciapa,
Structural modifications induced by TPA (12-O-tetradecanoyl phorbol-13-acetate) in sea urchin eggs.
1988,
Pubmed
Craven,
Increase in diacylglycerol mass in isolated glomeruli by glucose from de novo synthesis of glycerolipids.
1990,
Pubmed
Eckberg,
Diacylglycerol content of Chaetopterus oocytes during maturation and fertilization.
1993,
Pubmed
Exton,
Phosphatidylcholine breakdown and signal transduction.
1994,
Pubmed
Exton,
Signaling through phosphatidylcholine breakdown.
1990,
Pubmed
Grandin,
Intracellular pH and intracellular free calcium responses to protein kinase C activators and inhibitors in Xenopus eggs.
1991,
Pubmed
,
Xenbase
Grandin,
The increase in intracellular pH associated with Xenopus egg activation is a Ca(2+)-dependent wave.
1992,
Pubmed
,
Xenbase
Harrison,
Phosphoinositides and their products in the mammalian sperm acrosome reaction.
1990,
Pubmed
Kamel,
Phosphatidylinositol metabolism during fertilization in the sea urchin egg.
1985,
Pubmed
Kennerly,
Quantitative analysis of water-soluble products of cell-associated phospholipase C- and phospholipase D-catalyzed hydrolysis of phosphatidylcholine.
1991,
Pubmed
Khan,
Exogeneous diacylglycerols downregulate the activity of Na(+)-K+ pump in Xenopus laevis oocytes.
1991,
Pubmed
,
Xenbase
Kline,
Calcium-dependent events at fertilization of the frog egg: injection of a calcium buffer blocks ion channel opening, exocytosis, and formation of pronuclei.
1988,
Pubmed
,
Xenbase
Le Peuch,
Parthenogenetic activation decreases the polyphosphoinositide content of frog eggs.
1985,
Pubmed
,
Xenbase
Niemierko,
Action of phorbol myristate acetate (PMA) at fertilization of mouse oocytes in vitro.
1985,
Pubmed
Nishizuka,
Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C.
1992,
Pubmed
Olds,
Imaging protein kinase C activation in living sea urchin eggs after fertilization.
1995,
Pubmed
Raffaniello,
Protein kinase C expression and translocation in dispersed chief cells from guinea-pig stomach.
1994,
Pubmed
Sahara,
Characterization of protein kinase C in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Satterwhite,
Phosphorylation of myosin-II regulatory light chain by cyclin-p34cdc2: a mechanism for the timing of cytokinesis.
1992,
Pubmed
,
Xenbase
Shariff,
Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes.
1992,
Pubmed
Shea,
Technical report. An inexpensive densitometric analysis system using a Macintosh computer and a desktop scanner.
1994,
Pubmed
Shen,
A synthetic peptide of the pseudosubstrate domain of protein kinase C blocks cytoplasmic alkalinization during activation of the sea urchin egg.
1990,
Pubmed
Smith,
Inhibition of protein kinase C function by injection of intracellular receptors for the enzyme.
1992,
Pubmed
,
Xenbase
Stith,
Insulin and progesterone increase 32PO4-labeling of phospholipids and inositol 1,4,5-trisphosphate mass in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Stith,
Inositol 1,4,5-trisphosphate mass changes from fertilization through first cleavage in Xenopus laevis.
1993,
Pubmed
,
Xenbase
Stith,
Sperm increase inositol 1,4,5-trisphosphate mass in Xenopus laevis eggs preinjected with calcium buffers or heparin.
1994,
Pubmed
,
Xenbase
Stith,
Insulin-like growth factor 1, insulin, and progesterone induce early and late increases in Xenopus oocyte sn-1,2-diacylglycerol levels before meiotic cell division.
1991,
Pubmed
,
Xenbase
Swann,
Evidence for an inhibitory effect of protein kinase C on G-protein-mediated repetitive calcium transients in hamster eggs.
1989,
Pubmed
Turner,
Fertilization increases the polyphosphoinositide content of sea urchin eggs.
,
Pubmed
Vasilets,
Activation of protein kinase C by phorbol ester induces downregulation of the Na+/K(+)-ATPase in oocytes of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Whitaker,
How calcium may cause exocytosis in sea urchin eggs.
1987,
Pubmed
