Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1997 Mar 18;946:2301-5. doi: 10.1073/pnas.94.6.2301.
Show Gene links
Show Anatomy links
Feedback inhibition of Ca2+ channels by Ca2+ depends on a short sequence of the C terminus that does not include the Ca2+ -binding function of a motif with similarity to Ca2+ -binding domains.
Zhou J
,
Olcese R
,
Qin N
,
Noceti F
,
Birnbaumer L
,
Stefani E
.
???displayArticle.abstract???
alpha(1C)- and alpha(1E)-based Ca2+ channels differ in that the former are inhibited by Ca2+ entering through its pore, while the latter are not. It has been proposed on the basis of analysis of alpha(1E)/alpha(1C) chimeras that the molecular determinants responsible for Ca2+ inhibition involve both a conserved Ca2+-binding motif (EF hand) plus additional sequences located C-terminal to the EF hand. Through construction of similar alpha(1E)/alpha(1C) chimeras, we transferred Ca2+ inhibition from alpha(1C) to alpha(1E) by replacing a 134-aa segment of alpha(1E) with the homologous 142-aa segment of alpha(1C). This segment is located immediately after the proposed Ca2+ -binding EF hand motif. Replacement of the alpha(1C) EF hand with the corresponding EF hand of alpha(1E) did not interfere with inhibition of alpha(1C) by Ca2+, and a triple mutant of alpha(1C), alpha(1C)[D1535A,E1537A,D1546A], that disrupts the potential Ca2+-coordinating ability of the EF hand continued to be inhibited by Ca2+. These results indicate that a small portion of the alpha(1C) C terminus is essential for inhibition by Ca2+ and place the Ca2+ -binding site anywhere in alpha(1C), with the exception of its EF hand-like motif.
Babitch,
Channel hands.
1990,
Pubmed
Eckert,
Calcium-mediated inactivation of the calcium conductance in caesium-loaded giant neurones of Aplysia californica.
1981,
Pubmed
Flaherty,
Three-dimensional structure of recoverin, a calcium sensor in vision.
1993,
Pubmed
Imredy,
Submicroscopic Ca2+ diffusion mediates inhibitory coupling between individual Ca2+ channels.
1992,
Pubmed
Nakayama,
Evolution of EF-hand calcium-modulated proteins. II. Domains of several subfamilies have diverse evolutionary histories.
1992,
Pubmed
Neely,
Potentiation by the beta subunit of the ratio of the ionic current to the charge movement in the cardiac calcium channel.
1993,
Pubmed
,
Xenbase
Neely,
Ca(2+)-dependent inactivation of a cloned cardiac Ca2+ channel alpha 1 subunit (alpha 1C) expressed in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Olcese,
The amino terminus of a calcium channel beta subunit sets rates of channel inactivation independently of the subunit's effect on activation.
1994,
Pubmed
,
Xenbase
Perez-Reyes,
Cloning and expression of a cardiac/brain beta subunit of the L-type calcium channel.
1992,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Satyshur,
Refined structure of chicken skeletal muscle troponin C in the two-calcium state at 2-A resolution.
1988,
Pubmed
Schneider,
Molecular analysis and functional expression of the human type E neuronal Ca2+ channel alpha 1 subunit.
1994,
Pubmed
,
Xenbase
Taglialatela,
Gating currents of the cloned delayed-rectifier K+ channel DRK1.
1993,
Pubmed
,
Xenbase
Wei,
Heterologous regulation of the cardiac Ca2+ channel alpha 1 subunit by skeletal muscle beta and gamma subunits. Implications for the structure of cardiac L-type Ca2+ channels.
1991,
Pubmed
,
Xenbase
de Leon,
Essential Ca(2+)-binding motif for Ca(2+)-sensitive inactivation of L-type Ca2+ channels.
1995,
Pubmed