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XB-ART-17015
J Cell Biol January 27, 1997; 136 (2): 399-409.

A synthetic peptide corresponding to the extracellular domain of occludin perturbs the tight junction permeability barrier.

Wong V , Gumbiner BM .


Abstract
Occludin, the putative tight junction integral membrane protein, is an attractive candidate for a protein that forms the actual sealing element of the tight junction. To study the role of occludin in the formation of the tight junction seal, synthetic peptides (OCC1 and OCC2) corresponding to the two putative extracellular domains of occludin were assayed for their ability to alter tight junctions in Xenopus kidney epithelial cell line A6. Transepithelial electrical resistance and paracellular tracer flux measurements indicated that the second extracellular domain peptide (OCC2) reversibly disrupted the transepithelial permeability barrier at concentrations of < 5 microM. Despite the increased paracellular permeability, there were no changes in gross epithelial cell morphology as determined by scanning EM. The OCC2 peptide decreased the amount of occludin present at the tight junction, as assessed by indirect immunofluorescence, as well as decreased total cellular content of occludin, as assessed by Western blot analysis. Pulse-labeling and metabolic chase analysis suggested that this decrease in occludin level could be attributed to an increase in turnover of cellular occludin rather than a decrease in occludin synthesis. The effect on occludin was specific because other tight junction components, ZO-1, ZO-2, cingulin, and the adherens junction protein E-cadherin, were unaltered by OCC2 treatment. Therefore, the peptide corresponding to the second extracellular domain of occludin perturbs the tight junction permeability barrier in a very specific manner. The correlation between a decrease in occludin levels and the perturbation of the tight junction permeability barrier provides evidence for a role of occludin in the formation of the tight junction seal.

PubMed ID: 9015310
PMC ID: PMC2134825
Article link: J Cell Biol
Grant support: [+]
Genes referenced: cdh1 cgn ocln tjp1 tjp2


Article Images: [+] show captions
References [+] :
Anderson, Characterization of ZO-1, a protein component of the tight junction from mouse liver and Madin-Darby canine kidney cells. 1988, Pubmed


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