Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The human immunodeficiency virus type 1 Rev protein contains a nuclear export signal (NES) that is required for Rev-mediated RNA export in mammals as well as in the yeast Saccharomyces cerevisiae. The Rev NES has been shown to specifically interact with a human (hRIP/RAB1) and a yeast (yRip1p) protein in the two-hybrid assay. Both of these interacting proteins are related to FG nucleoporins on the basis of the presence of typical repeat motifs. This paper shows that Rev is able to interact with multiple FG repeat-containing nucleoporins from both S. cerevisiae and mammals; moreover, the ability of Rev NES mutants to interact with these FG nucleoporins parallels the ability of the mutants to promote RNA export in yeast and mammalian cells. The data also show that, after Xenopus oocyte nuclear injection, several FG nucleoporin repeat domains inhibit the export of both Rev protein and U small nuclear RNAs, suggesting that these nucleoporins participate in Rev-mediated and cellular RNA export. Interestingly, not all FG nucleoporin repeat domains produced the same pattern of RNA export inhibition. The results suggest that Rev and cellular mediators of RNA export can interact with multiple components of the nuclear pore complex during transport, analogous to the proposed mode of action of the nuclear protein import receptor.
Bevec,
Inhibition of HIV-1 replication in lymphocytes by mutants of the Rev cofactor eIF-5A.
1996, Pubmed
Bevec,
Inhibition of HIV-1 replication in lymphocytes by mutants of the Rev cofactor eIF-5A.
1996,
Pubmed
Bogerd,
Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins.
1995,
Pubmed
Cullen,
The HIV-1 Rev protein: prototype of a novel class of eukaryotic post-transcriptional regulators.
1991,
Pubmed
Davis,
The NUP1 gene encodes an essential component of the yeast nuclear pore complex.
1990,
Pubmed
Davis,
The nuclear pore complex.
1995,
Pubmed
,
Xenbase
Fabre,
Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif.
1994,
Pubmed
Finley,
Interaction mating reveals binary and ternary connections between Drosophila cell cycle regulators.
1994,
Pubmed
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Fischer,
Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA.
1994,
Pubmed
,
Xenbase
Fridell,
Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev.
1996,
Pubmed
,
Xenbase
Fridell,
Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathway.
1996,
Pubmed
Fritz,
HIV Rev uses a conserved cellular protein export pathway for the nucleocytoplasmic transport of viral RNAs.
1996,
Pubmed
Fritz,
A human nucleoporin-like protein that specifically interacts with HIV Rev.
1995,
Pubmed
Gorsch,
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
1995,
Pubmed
Grandi,
Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.
1995,
Pubmed
Gyuris,
Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2.
1993,
Pubmed
Görlich,
Nucleocytoplasmic transport.
1996,
Pubmed
Hurt,
A novel nucleoskeletal-like protein located at the nuclear periphery is required for the life cycle of Saccharomyces cerevisiae.
1988,
Pubmed
Iovine,
The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor.
1995,
Pubmed
Ito,
Transformation of intact yeast cells treated with alkali cations.
1983,
Pubmed
Ivey-Hoyle,
Rev-dependent expression of human immunodeficiency virus type 1 gp160 in Drosophila melanogaster cells.
1990,
Pubmed
Izaurralde,
RNA export.
1995,
Pubmed
Izaurralde,
A cap-binding protein complex mediating U snRNA export.
1995,
Pubmed
,
Xenbase
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Kambach,
Intracellular distribution of the U1A protein depends on active transport and nuclear binding to U1 snRNA.
1992,
Pubmed
,
Xenbase
Kraemer,
The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate.
1995,
Pubmed
Kraemer,
The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm.
1994,
Pubmed
Legrain,
Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm.
1989,
Pubmed
Loeb,
NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex.
1993,
Pubmed
Malim,
Mutational definition of the human immunodeficiency virus type 1 Rev activation domain.
1991,
Pubmed
Radu,
Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins.
1995,
Pubmed
,
Xenbase
Radu,
The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex.
1995,
Pubmed
Rexach,
Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins.
1995,
Pubmed
Rout,
Pores for thought: nuclear pore complex proteins.
1994,
Pubmed
Ruhl,
Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation.
1993,
Pubmed
,
Xenbase
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
Stutz,
Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast.
1995,
Pubmed
Stutz,
A functional interaction between Rev and yeast pre-mRNA is related to splicing complex formation.
1994,
Pubmed
Wen,
Identification of a signal for rapid export of proteins from the nucleus.
1995,
Pubmed
Wente,
A new family of yeast nuclear pore complex proteins.
1992,
Pubmed
Wente,
NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure.
1994,
Pubmed
Wimmer,
A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.
1992,
Pubmed
