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Nucleic Acids Res
1996 Nov 15;2422:4525-34. doi: 10.1093/nar/24.22.4525.
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Increasing the distance between the snRNA promoter and the 3' box decreases the efficiency of snRNA 3'-end formation.
Ramamurthy L
,
Ingledue TC
,
Pilch DR
,
Kay BK
,
Marzluff WF
.
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Chimeric genes which contained the mouse U1b snRNA promoter, portions of the histone H2a or globin coding regions and the U1b 3'-end followed by a histone 3'-end were constructed. The distance between the U1 promoter and the U1 3' box was varied between 146 and 670 nt. The chimeric genes were introduced into CHO cells by stable transfection or into Xenopus oocytes by microinjection. The efficiency of utilization of the U1 3' box, as measured by the relative amounts of transcripts that ended at the U1 3' box and the histone 3'-end, was dependent on the distance between the promoter and 3'-end box. U1 3'-ends were formed with >90% efficiency on transcripts shorter than 200 nt, with 50-70% efficiency on transcripts of 280-400 nt and with only 10-20% efficiency on transcripts >500 nt. Essentially identical results were obtained after stable transfection of CHO cells or after injecting the genes into Xenopus oocytes. The distance between the U1 promoter and the U1 3' box must be <280 nt for efficient transcription termination at the U1 3' box, regardless of the sequence transcribed.
Ach,
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Ach,
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,
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Bartlett,
Efficient expression of protein coding genes from the murine U1 small nuclear RNA promoters.
1996,
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Bentley,
Sequence requirements for premature termination of transcription in the human c-myc gene.
1988,
Pubmed
,
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Birnstiel,
Transcription termination and 3' processing: the end is in site!
1985,
Pubmed
Chaney,
High-frequency transfection of CHO cells using polybrene.
1986,
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Connelly,
Activity of chimeric U small nuclear RNA (snRNA)/mRNA genes in transfected protoplasts of Nicotiana plumbaginifolia: U snRNA 3'-end formation and transcription initiation can occur independently in plants.
1993,
Pubmed
Evans,
Biochemical fractionation of oocytes.
1991,
Pubmed
,
Xenbase
Graves,
Differential expression of two clusters of mouse histone genes.
1985,
Pubmed
Hernandez,
Elements required for transcription initiation of the human U2 snRNA gene coincide with elements required for snRNA 3' end formation.
1988,
Pubmed
Hernandez,
Formation of the 3' end of U1 snRNA is directed by a conserved sequence located downstream of the coding region.
1985,
Pubmed
Hernandez,
Formation of the 3' end of U1 snRNA requires compatible snRNA promoter elements.
1986,
Pubmed
Hurt,
The mouse histone H2a.2 gene from chromosome 3.
1989,
Pubmed
Kay,
Xenopus laevis: Practical uses in cell and molecular biology. Injections of oocytes and embryos.
1991,
Pubmed
,
Xenbase
Kephart,
Stability of Drosophila RNA polymerase II elongation complexes in vitro.
1992,
Pubmed
Kleinschmidt,
Accurate and efficient 3' processing of U2 small nuclear RNA precursor in a fractionated cytoplasmic extract.
1987,
Pubmed
Levine,
Differential expression of individual members of the histone multigene family due to sequences in the 5' and 3' regions of the genes.
1988,
Pubmed
Liu,
The efficiency of 3'-end formation contributes to the relative levels of different histone mRNAs.
1989,
Pubmed
Lobo,
Synthesis of U1 RNA in isolated mouse cell nuclei: initiation and 3'-end formation.
1987,
Pubmed
Lund,
In vitro synthesis of vertebrate U1 snRNA.
1989,
Pubmed
,
Xenbase
Marshall,
Control of formation of two distinct classes of RNA polymerase II elongation complexes.
1992,
Pubmed
Marzluff,
Isolation and characterization of two linked mouse U1b small nuclear RNA genes.
1983,
Pubmed
Middleton,
Premature termination of transcription can be induced on an injected alpha-tubulin gene in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Moussa,
A mouse Ulb-2 gene with extensive sequence similarity to a rat Ula gene for 670 nucleotides 5' to the gene.
1987,
Pubmed
Murphy,
Transcription of a gene for human U1 small nuclear RNA.
1982,
Pubmed
,
Xenbase
Neuman de Vegvar,
Nucleocytoplasmic transport and processing of small nuclear RNA precursors.
1990,
Pubmed
,
Xenbase
Parry,
The Xenopus U2 gene PSE is a single, compact, element required for transcription initiation and 3' end formation.
1989,
Pubmed
,
Xenbase
Patterson,
An essential yeast snRNA with a U5-like domain is required for splicing in vivo.
1987,
Pubmed
Pilch,
Expression of histone-U1 snRNA chimeric genes: U1 promoters are compatible with histone 3' end formation.
1991,
Pubmed
Roiha,
Small nuclear RNAs from budding yeasts: phylogenetic comparisons reveal extensive size variation.
1989,
Pubmed
Spencer,
Transcription elongation and eukaryotic gene regulation.
1990,
Pubmed
Sun,
The histone mRNA 3' end is required for localization of histone mRNA to polyribosomes.
1992,
Pubmed
Wendelburg,
Formation of the 3' end of sea urchin U1 small nuclear RNA occurs independently of the conserved 3' box and on transcripts initiated from a histone promoter.
1992,
Pubmed
Yang,
Nuclear processing of the 3'-terminal nucleotides of pre-U1 RNA in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
de Vegvar,
3' end formation of U1 snRNA precursors is coupled to transcription from snRNA promoters.
1986,
Pubmed
