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Genes Dev November 1, 1996; 10 (21): 2805-17.

Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos.

Hoppler S , Brown JD , Moon RT .

During gastrulation of Xenopus embryos the prospective mesoderm is induced initially with domains of dorsal and ventral fate, then further patterned to generate somitic mesoderm by signals from the gastrula organizer. Although Xwnt-8 is expressed in future ventrolateral mesoderm and induces prospective epidermis to differentiate in vitro as ventral mesoderm, no loss-of-function studies have demonstrated a requirement for Wnt signaling for the normal expression of mesodermal genes in the gastrula. We report development of a dominant-negative Wnt (dnXwnt-8) that inhibits embryonic responses to Wnt signaling in a cell-nonautonomous fashion. By expressing dnXwnt-8 in Xenopus embryos, we uncover a requirement of Wnt signaling for localized expression in prospective mesoderm of XMyoDa and Xenopus-posterior (Xpo). Because ectopic expression of functional Xwnt-8 in the dorsal marginal zone of the gastrula induces ectopic XMyoDa and Xpo, both gain-of-function and loss-of-function experiments support a model in which endogenous Xwnt-8 functions to induce expression of genes involved in specification of ventral and somitic mesoderm.

PubMed ID: 8946920
Article link: Genes Dev
Grant support: HD27525 NICHD NIH HHS , NIH2T32HDO7183-16 NICHD NIH HHS

Genes referenced: gsc hist1h4d myc myod1 nodal3.1 post wnt1 wnt8a xpo1

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