Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Direct and indirect association of the small GTPase ran with nuclear pore proteins and soluble transport factors: studies in Xenopus laevis egg extracts.
Saitoh H
,
Cooke CA
,
Burgess WH
,
Earnshaw WC
,
Dasso M
.
???displayArticle.abstract???
Ran is a small GTPase that is required for protein import, mRNA export, and the maintenance of nuclear structures. To gain a better understanding of Ran's role in the nucleus, we have sought to use Xenopus egg extracts for the purification and characterization of proteins from egg extracts bound with a high affinity to a glutathione-S-transferase-Ran fusion protein (GST-Ran). We found that GST-Ran associates specifically with at least 10 extract proteins. We determined the identifies of six Ran-interacting proteins (Rips), and found that they include RanBP2/Nup358, Nup153, Importin beta, hsc70, RCC1, and RanBP1. On the basis of peptide sequence, a seventh Rip (p88) seems to be similar but not identical to Fug1/RanGAP1, the mammalian Ran-GTPase-activating protein. Gel filtration analysis of endogenous extract proteins suggests that Importin beta acts as a primary GTP-Ran effector. Both Ran and Importin beta are coimmunoprecipitated by anti-p340RanBP2 antibodies in the presence of nonhydrolyzable GTP analogues, suggesting that Ran-Importin beta complexes interact with p340RanBP2. Two other Rips, p18 and p88, are coprecipitated with p340RanBP2 in a nucleotide-independent manner. Analysis of the Ran-GTPase pathway in Xenopus extracts allows the examination of interactions between Ran-associated proteins under conditions that resemble in vivo conditions more closely than in assays with purified components, and it thereby allows additional insights into the molecular mechanism of nuclear transport.
Ach,
A small nuclear GTP-binding protein from tomato suppresses a Schizosaccharomyces pombe cell-cycle mutant.
1994, Pubmed
Ach,
A small nuclear GTP-binding protein from tomato suppresses a Schizosaccharomyces pombe cell-cycle mutant.
1994,
Pubmed
Adam,
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.
1990,
Pubmed
,
Xenbase
Adam,
Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope.
1994,
Pubmed
Beddow,
The Ran/TC4 GTPase-binding domain: identification by expression cloning and characterization of a conserved sequence motif.
1995,
Pubmed
Bischoff,
Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.
1995,
Pubmed
Bischoff,
Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide.
1991,
Pubmed
Bischoff,
Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.
1995,
Pubmed
Byrd,
Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex.
1994,
Pubmed
Chi,
Sequence and characterization of cytoplasmic nuclear protein import factor p97.
1995,
Pubmed
Cooke,
CENP-B: a major human centromere protein located beneath the kinetochore.
1990,
Pubmed
Coutavas,
Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4.
1993,
Pubmed
,
Xenbase
Danscher,
Localization of gold in biological tissue. A photochemical method for light and electronmicroscopy.
1981,
Pubmed
Dasso,
RCC1, a regulator of mitosis, is essential for DNA replication.
1992,
Pubmed
,
Xenbase
Dasso,
A mutant form of the Ran/TC4 protein disrupts nuclear function in Xenopus laevis egg extracts by inhibiting the RCC1 protein, a regulator of chromosome condensation.
1994,
Pubmed
,
Xenbase
Davis,
Identification and characterization of a nuclear pore complex protein.
1986,
Pubmed
Davis,
The nuclear pore complex.
1995,
Pubmed
,
Xenbase
DeGregori,
A murine homolog of the yeast RNA1 gene is required for postimplantation development.
1994,
Pubmed
Drivas,
Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line.
1990,
Pubmed
Duan,
Inhibition of transcription elongation by the VHL tumor suppressor protein.
1995,
Pubmed
Finlay,
Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored.
1990,
Pubmed
,
Xenbase
Finlay,
A complex of nuclear pore proteins required for pore function.
1991,
Pubmed
Forbes,
Structure and function of the nuclear pore complex.
1992,
Pubmed
,
Xenbase
Guan,
Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase.
1991,
Pubmed
Görlich,
Distinct functions for the two importin subunits in nuclear protein import.
1995,
Pubmed
,
Xenbase
Görlich,
Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope.
1995,
Pubmed
,
Xenbase
Görlich,
Isolation of a protein that is essential for the first step of nuclear protein import.
1994,
Pubmed
,
Xenbase
Imamoto,
In vivo evidence for involvement of a 58 kDa component of nuclear pore-targeting complex in nuclear protein import.
1995,
Pubmed
,
Xenbase
Kornbluth,
Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression.
1994,
Pubmed
,
Xenbase
Lounsbury,
Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import.
1996,
Pubmed
Lounsbury,
A family of proteins that stabilize the Ran/TC4 GTPase in its GTP-bound conformation.
1994,
Pubmed
Macaulay,
Differential mitotic phosphorylation of proteins of the nuclear pore complex.
1995,
Pubmed
,
Xenbase
Meier,
Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation.
1995,
Pubmed
,
Xenbase
Melchior,
GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import.
1995,
Pubmed
Melchior,
Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor.
1993,
Pubmed
Melchior,
Mechanisms of nuclear protein import.
1995,
Pubmed
Moore,
Purification of a Ran-interacting protein that is required for protein import into the nucleus.
1994,
Pubmed
,
Xenbase
Moore,
The GTP-binding protein Ran/TC4 is required for protein import into the nucleus.
1993,
Pubmed
,
Xenbase
Moroianu,
Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins.
1995,
Pubmed
Moroianu,
Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.
1995,
Pubmed
Moroianu,
Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.
1995,
Pubmed
Newport,
Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
1987,
Pubmed
,
Xenbase
Paschal,
Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62.
1995,
Pubmed
Powers,
Cytosolic factors in nuclear transport: what's importin?
1994,
Pubmed
Radu,
Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins.
1995,
Pubmed
,
Xenbase
Ren,
Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing.
1995,
Pubmed
Rexach,
Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins.
1995,
Pubmed
Richards,
The C terminus of the nuclear RAN/TC4 GTPase stabilizes the GDP-bound state and mediates interactions with RCC1, RAN-GAP, and HTF9A/RANBP1.
1995,
Pubmed
Rout,
Pores for thought: nuclear pore complex proteins.
1994,
Pubmed
Rush,
The small nuclear GTPase Ran: how much does it run?
1996,
Pubmed
Saitoh,
The RCC1 protein interacts with Ran, RanBP1, hsc70, and a 340-kDa protein in Xenopus extracts.
1995,
Pubmed
,
Xenbase
Sazer,
The search for the primary function of the Ran GTPase continues.
1996,
Pubmed
Schlenstedt,
The GTP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(A)+ RNA in the cytoplasm.
1995,
Pubmed
Smythe,
Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in Xenopus laevis egg extracts.
1991,
Pubmed
,
Xenbase
Sukegawa,
A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm.
1993,
Pubmed
Weis,
Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences.
1995,
Pubmed
Wu,
Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region.
1995,
Pubmed
Yokoyama,
A giant nucleopore protein that binds Ran/TC4.
1995,
Pubmed
