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SMC proteins constitute two subunits of the mammalian recombination complex RC-1.
Jessberger R
,
Riwar B
,
Baechtold H
,
Akhmedov AT
.
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Recombination protein complex RC-1, purified from calf thymus nuclear extracts, catalyzes cell-free DNA strand transfer and repair of gaps and deletions through DNA recombination. DNA polymerase E, DNA ligase III and a DNA structure-specific endonuclease co-purify with the five polypeptide complex. Here we describe the identification of two hitherto unknown subunits of RC-1. N-terminal amino acid sequences of the 160 and 130 kDa polypeptides display up to 100% identity to proteins of the structural maintenance of chromosomes (SMC) subfamilies 1 and 2. SMC proteins are involved in mitotic chromosome segregation and condensation, as well as in certain DNA repair pathways in fission (rad18 gene) and budding (RHC18 gene) yeast. The assignment was substantiated by immuno-cross-reactivity of the RC-1 subunits with polyclonal antibodies specific for Xenopus laevis SMC proteins. These antibodies, and polyclonal antibodies directed against the bovine 160 and 130 kDa polypeptides, named BSMC1 and BSMC2 (bovine SMC), inhibited RC-1-mediated DNA transfer, indicating that the SMC proteins are necessary components of the reaction. Two independent assays revealed DNA reannealing activity of RC-1, which resides in its BSMC subunits, thereby demonstrating a novel function of these proteins. To our knowledge, this is the first evidence for the association of mammalian SMC proteins with a multiprotein complex harboring, among others, DNA recombination, DNA ligase and DNA polymerase activities.
Benson,
Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA.
1994, Pubmed
Benson,
Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA.
1994,
Pubmed
Bezzubova,
Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes.
1993,
Pubmed
Bezzubova,
Gene conversion in the chicken immunoglobulin locus: a paradigm of homologous recombination in higher eukaryotes.
1994,
Pubmed
Blunt,
Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation.
1995,
Pubmed
Bryant,
On the mechanism of renaturation of complementary DNA strands by the recA protein of Escherichia coli.
1985,
Pubmed
Chant,
GTPase cascades choreographing cellular behavior: movement, morphogenesis, and more.
1995,
Pubmed
Chuang,
DPY-27:a chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome.
1994,
Pubmed
,
Xenbase
Gellert,
Molecular analysis of V(D)J recombination.
1992,
Pubmed
Hirano,
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.
1994,
Pubmed
,
Xenbase
Hirano,
The SMC family: from chromosome condensation to dosage compensation.
1995,
Pubmed
,
Xenbase
Hirano,
Biochemical and genetic dissection of mitotic chromosome condensation.
1995,
Pubmed
Jessberger,
A mammalian protein complex that repairs double-strand breaks and deletions by recombination.
1993,
Pubmed
Jessberger,
Stimulation of defective DNA transfer activity in recombination deficient SCID cell extracts by a 72-kDa protein from wild-type thymocytes.
1995,
Pubmed
Jessberger,
Analysis of the mammalian recombination protein complex RC-1.
1996,
Pubmed
Kirchgessner,
DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect.
1995,
Pubmed
Kowalczykowski,
Homologous pairing and DNA strand-exchange proteins.
1994,
Pubmed
Lehmann,
The rad18 gene of Schizosaccharomyces pombe defines a new subgroup of the SMC superfamily involved in DNA repair.
1995,
Pubmed
Lindahl,
Mammalian DNA ligases.
1992,
Pubmed
McBlane,
Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps.
1995,
Pubmed
Nasim,
Genetic control of radiation sensitivity in Schizosaccharomyces pombe.
1975,
Pubmed
Peterson,
The SMC family: novel motor proteins for chromosome condensation?
1994,
Pubmed
Rocques,
The human SB1.8 gene (DXS423E) encodes a putative chromosome segregation protein conserved in lower eukaryotes and prokaryotes.
1995,
Pubmed
Saitoh,
The SMC proteins and the coming of age of the chromosome scaffold hypothesis.
1995,
Pubmed
,
Xenbase
Saitoh,
ScII: an abundant chromosome scaffold protein is a member of a family of putative ATPases with an unusual predicted tertiary structure.
1994,
Pubmed
Saka,
Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis.
1994,
Pubmed
Shinohara,
Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA.
1993,
Pubmed
Strunnikov,
SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family.
1993,
Pubmed
Strunnikov,
SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.
1995,
Pubmed
Weinstock,
ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli.
1979,
Pubmed
West,
Enzymes and molecular mechanisms of genetic recombination.
1992,
Pubmed
Wilson,
2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans.
1994,
Pubmed
