Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Xenopus embryonic cell adhesion to fibronectin: position-specific activation of RGD/synergy site-dependent migratory behavior at gastrulation.
Ramos JW
,
DeSimone DW
.
???displayArticle.abstract???
During Xenopus laevis gastrulation, the basic body plan of the embryo is generated by movement of the marginal zone cells of the blastula into the blastocoel cavity. This morphogenetic process involves cell adhesion to the extracellular matrix protein fibronectin (FN). Regions of FN required for the attachment and migration of involuting marginal zone (IMZ) cells were analyzed in vitro using FN fusion protein substrates. IMZ cell attachment to FN is mediated by the Arg-Gly-Asp (RGD) sequence located in the type III-10 repeat and by the Pro-Pro-Arg-Arg-Ala-Arg (PPRRAR) sequence in the type III-13 repeat of the Hep II domain. IMZ cells spread and migrate persistently on fusion proteins containing both the RGD and synergy site sequence Pro-Pro-Ser-Arg-Asn (PPSRN) located in the type III-9 repeat. Cell recognition of the synergy site is positionally regulated in the early embryo. During gastrulation, IMZ cells will spread and migrate on FN whereas presumptive pre-involuting mesoderm, vegetal pole endoderm, and animal cap ectoderm will not. However, animal cap ectoderm cells acquire the ability to spread and migrate on the RGD/synergy region when treated with the mesoderm inducing factor activin-A. These data suggest that mesoderm induction activates the position-specific recognition of the synergy site of FN in vivo. Moreover, we demonstrate the functional importance of this site using a monoclonal antibody that blocks synergy region-dependent cell spreading and migration on FN. Normal IMZ movement is perturbed when this antibody is injected into the blastocoel cavity indicating that IMZ cell interaction with the synergy region is required for normal gastrulation.
Aota,
The short amino acid sequence Pro-His-Ser-Arg-Asn in human fibronectin enhances cell-adhesive function.
1994, Pubmed
Aota,
The short amino acid sequence Pro-His-Ser-Arg-Asn in human fibronectin enhances cell-adhesive function.
1994,
Pubmed
Barkalow,
Localization of the major heparin-binding site in fibronectin.
1991,
Pubmed
Boucaut,
Biologically active synthetic peptides as probes of embryonic development: a competitive peptide inhibitor of fibronectin function inhibits gastrulation in amphibian embryos and neural crest cell migration in avian embryos.
1984,
Pubmed
Boucaut,
Prevention of gastrulation but not neurulation by antibodies to fibronectin in amphibian embryos.
,
Pubmed
Bowditch,
Identification of a novel integrin binding site in fibronectin. Differential utilization by beta 3 integrins.
1994,
Pubmed
Brickman,
Heparitinase inhibition of mesoderm induction and gastrulation in Xenopus laevis embryos.
1994,
Pubmed
,
Xenbase
Busby,
Heparin binding by fibronectin module III-13 involves six discontinuous basic residues brought together to form a cationic cradle.
1995,
Pubmed
Chan,
T cell receptor-dependent, antigen-specific stimulation of a murine T cell clone induces a transient, VLA protein-mediated binding to extracellular matrix.
1991,
Pubmed
Chapman,
Recognition of two Dermatophagoides pteronyssinus-specific epitopes on antigen P1 by using monoclonal antibodies: binding to each epitope can be inhibited by serum from dust mite-allergic patients.
1984,
Pubmed
Danen,
Requirement for the synergy site for cell adhesion to fibronectin depends on the activation state of integrin alpha 5 beta 1.
1995,
Pubmed
Dawid,
Axis determination in Xenopus: gradients and signals.
1994,
Pubmed
,
Xenbase
DeSimone,
Identification and characterization of alternatively spliced fibronectin mRNAs expressed in early Xenopus embryos.
1992,
Pubmed
,
Xenbase
DeSimone,
The Xenopus embryo as a model system for the study of cell-extracellular matrix interactions.
1991,
Pubmed
,
Xenbase
DeSimone,
Xenopus laevis integrins. Structural conservation and evolutionary divergence of integrin beta subunits.
1988,
Pubmed
,
Xenbase
Drake,
Cell surface phosphatidylinositol-anchored heparan sulfate proteoglycan initiates mouse melanoma cell adhesion to a fibronectin-derived, heparin-binding synthetic peptide.
1992,
Pubmed
Garcia-Pardo,
Human B lymphocytes define an alternative mechanism of adhesion to fibronectin. The interaction of the alpha 4 beta 1 integrin with the LHGPEILDVPST sequence of the type III connecting segment is sufficient to promote cell attachment.
1990,
Pubmed
George,
Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin.
1993,
Pubmed
Guan,
Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase.
1991,
Pubmed
Guan,
Lymphoid cells recognize an alternatively spliced segment of fibronectin via the integrin receptor alpha 4 beta 1.
1990,
Pubmed
Gurdon,
Methods for nuclear transplantation in amphibia.
1977,
Pubmed
Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase
Haugen,
Recognition of the A chain carboxy-terminal heparin binding region of fibronectin involves multiple sites: two contiguous sequences act independently to promote neural cell adhesion.
1990,
Pubmed
Hemsley,
A simple method for site-directed mutagenesis using the polymerase chain reaction.
1989,
Pubmed
Hoogenraad,
The effect of pristane on ascites tumor formation and monoclonal antibody production.
1986,
Pubmed
Howard,
Are beta 1 integrins involved in Xenopus gastrulation?
1992,
Pubmed
,
Xenbase
Howard,
Analysis of gastrulation: different types of gastrulation movement are induced by different mesoderm-inducing factors in Xenopus laevis.
1993,
Pubmed
,
Xenbase
Humphries,
Identification of two distinct regions of the type III connecting segment of human plasma fibronectin that promote cell type-specific adhesion.
1987,
Pubmed
Hynes,
Integrins: versatility, modulation, and signaling in cell adhesion.
1992,
Pubmed
Joos,
Integrin alpha 5 during early development of Xenopus laevis.
1995,
Pubmed
,
Xenbase
Keller,
Cellular basis of amphibian gastrulation.
1992,
Pubmed
Komoriya,
The minimal essential sequence for a major cell type-specific adhesion site (CS1) within the alternatively spliced type III connecting segment domain of fibronectin is leucine-aspartic acid-valine.
1991,
Pubmed
Lee,
Temporal and spatial regulation of fibronectin in early Xenopus development.
1984,
Pubmed
,
Xenbase
McCarthy,
Localization and chemical synthesis of fibronectin peptides with melanoma adhesion and heparin binding activities.
1988,
Pubmed
Mould,
The CS5 peptide is a second site in the IIICS region of fibronectin recognized by the integrin alpha 4 beta 1. Inhibition of alpha 4 beta 1 function by RGD peptide homologues.
1991,
Pubmed
Nakatsuji,
Conditioning of a culture substratum by the ectodermal layer promotes attachment and oriented locomotion by amphibian gastrula mesodermal cells.
1983,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Niehrs,
The homeobox gene goosecoid controls cell migration in Xenopus embryos.
1993,
Pubmed
,
Xenbase
Pierschbacher,
Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule.
,
Pubmed
Pytela,
Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor.
1985,
Pubmed
Ransom,
Integrin expression in early amphibian embryos: cDNA cloning and characterization of Xenopus beta 1, beta 2, beta 3, and beta 6 subunits.
1993,
Pubmed
,
Xenbase
Ruoslahti,
Proteoglycans in cell regulation.
1989,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Saunders,
Cell surface proteoglycan binds mouse mammary epithelial cells to fibronectin and behaves as a receptor for interstitial matrix.
1988,
Pubmed
Schwarzbauer,
Alternative splicing of fibronectin: three variants, three functions.
1991,
Pubmed
Sekiguchi,
Functional domain structure of fibronectin.
1980,
Pubmed
Shih,
The epithelium of the dorsal marginal zone of Xenopus has organizer properties.
1992,
Pubmed
,
Xenbase
Shimizu,
Regulated expression and binding of three VLA (beta 1) integrin receptors on T cells.
1990,
Pubmed
Skorstengaard,
Purification and complete primary structures of the heparin-, cell-, and DNA-binding domains of bovine plasma fibronectin.
1986,
Pubmed
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
Smith,
Purification, partial characterization and biological effects of the XTC mesoderm-inducing factor.
1988,
Pubmed
,
Xenbase
Smith,
Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction.
1991,
Pubmed
,
Xenbase
Smith,
Mesoderm induction and the control of gastrulation in Xenopus laevis: the roles of fibronectin and integrins.
1990,
Pubmed
,
Xenbase
Sokol,
The pregastrula establishment of gene expression pattern in Xenopus embryos: requirements for local cell interactions and for protein synthesis.
1994,
Pubmed
,
Xenbase
Wayner,
Activation-dependent recognition by hematopoietic cells of the LDV sequence in the V region of fibronectin.
1992,
Pubmed
Whittaker,
Integrin alpha subunit mRNAs are differentially expressed in early Xenopus embryos.
1993,
Pubmed
,
Xenbase
Wight,
The role of proteoglycans in cell adhesion, migration and proliferation.
1992,
Pubmed
Williams,
The inner world of cell adhesion: integrin cytoplasmic domains.
1994,
Pubmed
Winklbauer,
Cell interaction and its role in mesoderm cell migration during Xenopus gastrulation.
1992,
Pubmed
,
Xenbase
Winklbauer,
Differential interaction of Xenopus embryonic cells with fibronectin in vitro.
1988,
Pubmed
,
Xenbase
Winklbauer,
Mesodermal cell migration during Xenopus gastrulation.
1990,
Pubmed
,
Xenbase
Winklbauer,
Motile behavior and protrusive activity of migratory mesoderm cells from the Xenopus gastrula.
1992,
Pubmed
,
Xenbase
Woods,
Adhesion and cytoskeletal organisation of fibroblasts in response to fibronectin fragments.
1986,
Pubmed
Wu,
Integrin activation and cytoskeletal interaction are essential for the assembly of a fibronectin matrix.
1995,
Pubmed
Yamada,
Fibronectins: structure, functions and receptors.
1989,
Pubmed
