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Development July 1, 1996; 122 (7): 2099-108.

Mothers against dpp encodes a conserved cytoplasmic protein required in DPP/TGF-beta responsive cells.

Newfeld SJ , Chartoff EH , Graff JM , Melton DA , Gelbart WM .

The proteins necessary for signal transduction in cells responding to ligands of the TGF-beta family are largely unknown. We have previously identified Mad (Mothers against dpp), a gene that interacts with the TGF-beta family member encoded by decapentaplegic (dpp) in Drosophila. Assay of Mad''s role in the DPP-dependent events of embryonic midgut development demonstrates that Mad is required for any response of the visceral mesoderm or endoderm to DPP signals from the visceral mesoderm. Replacement of the normal DPP promoter with a heterologous (hsp70) promoter fails to restore DPP-dependent responses in Mad mutant midguts. Experiments utilizing Mad transgenes regulated by tissue-specific promoters show that MAD is required specifically in cells responding to DPP. Immunohistochemical studies localize MAD to the cytoplasm in all tissues examined. Experiments in Xenopus embryos demonstrate that Drosophila MAD can function in the signaling pathway of BMP-4, a vertebrate homolog of dpp. Based on these results, we propose that Mad is a highly conserved and essential element of the DPP signal transduction pathway.

PubMed ID: 8681791
Article link: Development

Genes referenced: actl6a bmp4 dspp hsp70 hspa1l ncam1 tbx2 tgfb1

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