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Cell cycle regulation of dynein association with membranes modulates microtubule-based organelle transport.
Niclas J
,
Allan VJ
,
Vale RD
.
???displayArticle.abstract??? Cytoplasmic dynein is a minus end-directed microtubule motor that performs distinct functions in interphase and mitosis. In interphase, dynein transports organelles along microtubules, whereas in metaphase this motor has been implicated in mitotic spindle formation and orientation as well as chromosome segregation. The manner in which dynein activity is regulated during the cell cycle, however, has not been resolved. In this study, we have examined the mechanism by which organelle transport is controlled by the cell cycle in extracts of Xenopus laevis eggs. Here, we show that photocleavage of the dynein heavy chain dramatically inhibits minus end-directed organelle transport and that purified dynein restores this motility, indicating that dynein is the predominant minus end-directed membrane motor in Xenopus egg extracts. By measuring the amount of dynein associated with isolated membranes, we find that cytoplasmic dynein and its activator dynactin detach from the membrane surface in metaphase extracts. The sevenfold decrease in membrane-associated dynein correlated well with the eightfold reduction in minus end-directed membrane transport observed in metaphase versus interphase extracts. Although dynein heavy or intermediate chain phosphorylation did not change in a cell cycle-dependent manner, the dynein light intermediate chain incorporated approximately 12-fold more radiolabeled phosphate in metaphase than in interphase extracts. These studies suggest that cell cycle-dependent phosphorylation of cytoplasmic dynein may regulate organelle transport by modulating the association of this motor with membranes.
Allan,
Protein phosphatase 1 regulates the cytoplasmic dynein-driven formation of endoplasmic reticulum networks in vitro.
1995, Pubmed,
Xenbase
Allan,
Protein phosphatase 1 regulates the cytoplasmic dynein-driven formation of endoplasmic reticulum networks in vitro.
1995,
Pubmed
,
Xenbase
Allan,
Cell cycle control of microtubule-based membrane transport and tubule formation in vitro.
1991,
Pubmed
,
Xenbase
Brady,
A novel brain ATPase with properties expected for the fast axonal transport motor.
,
Pubmed
Dillman,
Differential phosphorylation in vivo of cytoplasmic dynein associated with anterogradely moving organelles.
1994,
Pubmed
Echeverri,
Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis.
1996,
Pubmed
Eshel,
Cytoplasmic dynein is required for normal nuclear segregation in yeast.
1993,
Pubmed
Gibbons,
Photosensitized cleavage of dynein heavy chains. Cleavage at the "V1 site" by irradiation at 365 nm in the presence of ATP and vanadate.
1987,
Pubmed
Gill,
Characterization of DLC-A and DLC-B, two families of cytoplasmic dynein light chain subunits.
1994,
Pubmed
Gill,
Dynactin, a conserved, ubiquitously expressed component of an activator of vesicle motility mediated by cytoplasmic dynein.
1991,
Pubmed
Hamm-Alvarez,
Regulation of vesicle transport in CV-1 cells and extracts.
1993,
Pubmed
Hollenbeck,
Phosphorylation of neuronal kinesin heavy and light chains in vivo.
1993,
Pubmed
Hollenbeck,
Radial extension of macrophage tubular lysosomes supported by kinesin.
1990,
Pubmed
Hughes,
Molecular analysis of a cytoplasmic dynein light intermediate chain reveals homology to a family of ATPases.
1995,
Pubmed
Karki,
Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex.
1995,
Pubmed
King,
Localization of an intermediate chain of outer arm dynein by immunoelectron microscopy.
1990,
Pubmed
King,
The Mr 78,000 intermediate chain of Chlamydomonas outer arm dynein interacts with alpha-tubulin in situ.
1991,
Pubmed
Koonce,
Dynein from Dictyostelium: primary structure comparisons between a cytoplasmic motor enzyme and flagellar dynein.
1992,
Pubmed
Kotz,
Intracellular calcium and cAMP regulate directional pigment movements in teleost erythrophores.
1994,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lee,
Phosphorylation of kinesin in vivo correlates with organelle association and neurite outgrowth.
1995,
Pubmed
Lee-Eiford,
Specific cleavage of dynein heavy chains by ultraviolet irradiation in the presence of ATP and vanadate.
1986,
Pubmed
Li,
Disruption of mitotic spindle orientation in a yeast dynein mutant.
1993,
Pubmed
Lin,
Cytoplasmic dynein undergoes intracellular redistribution concomitant with phosphorylation of the heavy chain in response to serum starvation and okadaic acid.
1994,
Pubmed
Lynch,
Regulation of pigment organelle translocation. I. Phosphorylation of the organelle-associated protein p57.
1986,
Pubmed
Lynch,
Regulation of pigment organelle translocation. II. Participation of a cAMP-dependent protein kinase.
1986,
Pubmed
Matthies,
Calmodulin binding to and cAMP-dependent phosphorylation of kinesin light chains modulate kinesin ATPase activity.
1993,
Pubmed
McIlvain,
Regulation of kinesin activity by phosphorylation of kinesin-associated proteins.
1994,
Pubmed
Melloni,
Expression of the p150Glued component of the dynactin complex in developing and adult rat brain.
1995,
Pubmed
Mitchell,
Protein-protein interactions in the 18S ATPase of Chlamydomonas outer dynein arms.
1986,
Pubmed
Muhua,
A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration.
1994,
Pubmed
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Murray,
Cell cycle extracts.
1991,
Pubmed
Niclas,
Cloning and localization of a conventional kinesin motor expressed exclusively in neurons.
1994,
Pubmed
Okada,
The activation of protein kinase A pathway selectively inhibits anterograde axonal transport of vesicles but not mitochondria transport or retrograde transport in vivo.
1995,
Pubmed
Palazzo,
Centriole duplication in lysates of Spisula solidissima oocytes.
1992,
Pubmed
Paschal,
MAP 1C is a microtubule-activated ATPase which translocates microtubules in vitro and has dynein-like properties.
1987,
Pubmed
Paschal,
Retrograde transport by the microtubule-associated protein MAP 1C.
,
Pubmed
Paschal,
Homology of the 74-kD cytoplasmic dynein subunit with a flagellar dynein polypeptide suggests an intracellular targeting function.
1992,
Pubmed
Pfarr,
Cytoplasmic dynein is localized to kinetochores during mitosis.
1990,
Pubmed
Plamann,
Cytoplasmic dynein and actin-related protein Arp1 are required for normal nuclear distribution in filamentous fungi.
1994,
Pubmed
Rodionov,
Kinesin is responsible for centrifugal movement of pigment granules in melanophores.
1991,
Pubmed
Rozdzial,
Bidirectional pigment granule movements of melanophores are regulated by protein phosphorylation and dephosphorylation.
1986,
Pubmed
Saunders,
Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation.
1995,
Pubmed
Schnapp,
Dynein is the motor for retrograde axonal transport of organelles.
1989,
Pubmed
Schroer,
Cytoplasmic dynein is a minus end-directed motor for membranous organelles.
1989,
Pubmed
Schroer,
Two activators of microtubule-based vesicle transport.
1991,
Pubmed
Steuer,
Localization of cytoplasmic dynein to mitotic spindles and kinetochores.
1990,
Pubmed
Thaler,
Regulation of organelle transport in melanophores by calcineurin.
1990,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Vaisberg,
Cytoplasmic dynein plays a role in mammalian mitotic spindle formation.
1993,
Pubmed
Vale,
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
1985,
Pubmed
Vaughan,
Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued.
1995,
Pubmed
Verde,
Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein.
1991,
Pubmed
,
Xenbase
Warren,
Organelle inheritance.
1996,
Pubmed
Warren,
Newly synthesized G protein of vesicular stomatitis virus is not transported to the cell surface during mitosis.
1983,
Pubmed
Warren,
Recycling of transferrin receptors in A431 cells is inhibited during mitosis.
1984,
Pubmed
Xiang,
Cytoplasmic dynein is involved in nuclear migration in Aspergillus nidulans.
1994,
Pubmed
