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Proc Natl Acad Sci U S A
1996 Apr 16;938:3684-8. doi: 10.1073/pnas.93.8.3684.
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P2X4: an ATP-activated ionotropic receptor cloned from rat brain.
Soto F
,
Garcia-Guzman M
,
Gomez-Hernandez JM
,
Hollmann M
,
Karschin C
,
Stühmer W
.
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Extracellular ATP exerts pronounced biological actions in virtually every organ or tissue that has been studied. In the central and peripheral nervous system, ATP acts as a fast excitatory transmitter in certain synaptic pathways [Evans, R.J., Derkach, V. & Surprenant, A. (1992) Nature (London) 357, 503-505; Edwards, F.A., Gigg, A.J. & Colquhoun, D. (1992) Nature (London) 359, 144-147]. Here, we report the cloning and characterization of complementary DNA from rat brain, encoding an additional member (P2X4) of the emerging multigenic family of ligand-gated ATP channels, the P2X receptors. Expression in Xenopus oocytes gives an ATP-activated cation-selective channel that is highly permeable to Ca2+ and whose sensitivity is modulated by extracellular Zn2+. Surprisingly, the current elicited by ATP is almost insensitive to the common P2X antagonist suramin. In situ hybridization reveals the expression of P2X4 mRNA in central nervous system neurons. Northern blot and reverse transcription-PCR (RT-PCR) analysis demonstrate a wide distribution of P2X4 transcripts in various tissues, including blood vessels and leukocytes. This suggests that the P2X4 receptor might mediate not only ATP-dependent synaptic transmission in the central nervous system but also a wide repertoire of biological responses in diverse tissues.
Abbracchio,
Purinoceptors: are there families of P2X and P2Y purinoceptors?
1994, Pubmed
Abbracchio,
Purinoceptors: are there families of P2X and P2Y purinoceptors?
1994,
Pubmed
Bartsch,
Expression of tenascin in the developing and adult cerebellar cortex.
1992,
Pubmed
Bean,
Pharmacology and electrophysiology of ATP-activated ion channels.
1992,
Pubmed
Benham,
ATP-activated channels gate calcium entry in single smooth muscle cells dissociated from rabbit ear artery.
1989,
Pubmed
Bo,
Heterogeneous distribution of [3H]alpha,beta-methylene ATP binding sites in blood vessels.
1993,
Pubmed
Bo,
A P2X purinoceptor cDNA conferring a novel pharmacological profile.
1995,
Pubmed
Brake,
New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor.
1994,
Pubmed
,
Xenbase
Buell,
An antagonist-insensitive P2X receptor expressed in epithelia and brain.
1996,
Pubmed
Burnashev,
Fractional calcium currents through recombinant GluR channels of the NMDA, AMPA and kainate receptor subtypes.
1995,
Pubmed
Chen,
Nucleotides as extracellular signalling molecules.
1995,
Pubmed
Chen,
A P2X purinoceptor expressed by a subset of sensory neurons.
1995,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Cloues,
Zn2+ potentiates ATP-activated currents in rat sympathetic neurons.
1993,
Pubmed
Dubyak,
Signal transduction via P2-purinergic receptors for extracellular ATP and other nucleotides.
1993,
Pubmed
Edwards,
ATP--a fast neurotransmitter.
1993,
Pubmed
Egebjerg,
Ca2+ permeability of unedited and edited versions of the kainate selective glutamate receptor GluR6.
1993,
Pubmed
,
Xenbase
Evans,
Pharmacological characterization of heterologously expressed ATP-gated cation channels (P2x purinoceptors).
1995,
Pubmed
Frederickson,
Neurobiology of zinc and zinc-containing neurons.
1989,
Pubmed
Hollmann,
Ca2+ permeability of KA-AMPA--gated glutamate receptor channels depends on subunit composition.
1991,
Pubmed
,
Xenbase
Hollmann,
Zinc potentiates agonist-induced currents at certain splice variants of the NMDA receptor.
1993,
Pubmed
,
Xenbase
Iino,
Permeation of calcium through excitatory amino acid receptor channels in cultured rat hippocampal neurones.
1990,
Pubmed
Kennedy,
How should P2X purinoceptors be classified pharmacologically?
1995,
Pubmed
Lewis,
Coexpression of P2X2 and P2X3 receptor subunits can account for ATP-gated currents in sensory neurons.
1995,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Mayer,
Permeation and block of N-methyl-D-aspartic acid receptor channels by divalent cations in mouse cultured central neurones.
1987,
Pubmed
Nakazawa,
Effects of ATP antagonists on purinoceptor-operated inward currents in rat phaeochromocytoma cells.
1991,
Pubmed
Naumov,
ATP-activated inward current and calcium-permeable channels in rat macrophage plasma membranes.
1995,
Pubmed
Saraste,
The P-loop--a common motif in ATP- and GTP-binding proteins.
1990,
Pubmed
Shatkay,
Individual activity of calcium ions in pure solutions of CaCl2 and in mixtures.
1968,
Pubmed
Stühmer,
Electrophysiological recording from Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Surprenant,
P2X receptors bring new structure to ligand-gated ion channels.
1995,
Pubmed
Séguéla,
A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain.
1996,
Pubmed
,
Xenbase
Valera,
A new class of ligand-gated ion channel defined by P2x receptor for extracellular ATP.
1994,
Pubmed
,
Xenbase
Ziganshin,
Selective antagonism by PPADS at P2X-purinoceptors in rabbit isolated blood vessels.
1994,
Pubmed
