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XB-ART-184
Dev Dyn. August 1, 2006; 235 (8): 2220-8.

Transgenic Xenopus laevis strain expressing cre recombinase in muscle cells.

Waldner C , Sakamaki K , Ueno N , Turan G , Ryffel GU .


Abstract
For reproducible analyses of gene function in Xenopus, the use of transgenic strains is a promising approach but has limitations when investigating factors interfering with development. Therefore, inducible systems are attractive alternatives, and a binary system based on recombinases is a most versatile approach. We have shown previously that Cre and FLP recombinases are active in Xenopus laevis and can induce a silent reporter gene in a corresponding reporter strain. Here, we describe the establishment of the transgenic Xenopus laevis strain A7 expressing Cre recombinase under the control of the muscle-specific cardiac actin promoter. Upon crossing to several distinct reporter strains, A7 is able to induce EYFP, DsRed2, or LacZ reporter genes in a muscle-specific manner. This first Cre-expressing strain allows conditional activation of any gene of interest in muscle cells and, thus, opens up the use of recombinases as a new experimental strategy in Xenopus.

PubMed ID: 16804894
Article link: Dev Dyn.

Genes referenced: actc1
Antibodies referenced:
Morpholinos referenced:
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