Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Effects of cytochalasin treatment on short-term synaptic plasticity at developing neuromuscular junctions in frogs.
Wang XH
,
Zheng JQ
,
Poo MM
.
???displayArticle.abstract???
1. The role of actin microfilaments in synaptic transmission was tested by monitoring spontaneous and evoked transmitter release from developing neuromuscular synapses in Xenopus nerve-muscle cultures, using whole-cell recording of synaptic currents in the absence and presence of microfilament-disrupting agents cytochalasins B and D. 2. Treatment with cytochalasins resulted in disruption of microfilament networks in the growth cone and the presynaptic nerve terminal of spinal neurons in Xenopus nerve-muscle cultures, as revealed by rhodamine-phalloidin staining. 3. The same cytochalasin treatment did not significantly affect the spontaneous or evoked synaptic currents during low-frequency stimulation at 0.05 Hz in these Xenopus cultures. Synaptic depression induced by high-frequency (5 Hz) stimulation, however, was reduced by this treatment. Paired-pulse facilitation for short interpulse intervals was also increased by the treatment. 4. These results indicate that disruption of microfilaments alters short-term changes in transmitter release induced by repetitive activity, without affecting normal synaptic transmission at low frequency. 5. Our results support the notion that actin microfilaments impose a barrier for mobilization of synaptic vesicles from the reserve pool, but do not affect the exocytosis of immediately available synaptic vesicles at the active zone.
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977, Pubmed,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Aunis,
The cytoskeleton as a barrier to exocytosis in secretory cells.
1988,
Pubmed
Benfenati,
Interaction of free and synaptic vesicle-bound synapsin I with F-actin.
1992,
Pubmed
Bernstein,
Cycling of actin assembly in synaptosomes and neurotransmitter release.
1989,
Pubmed
Betz,
Okadaic acid disrupts clusters of synaptic vesicles in frog motor nerve terminals.
1994,
Pubmed
Bonder,
Cytochalasin B slows but does not prevent monomer addition at the barbed end of the actin filament.
1986,
Pubmed
Cooper,
Effects of cytochalasin and phalloidin on actin.
1987,
Pubmed
De Camilli,
The synapsins.
1990,
Pubmed
Evers,
Studies of nerve-muscle interactions in Xenopus cell culture: analysis of early synaptic currents.
1989,
Pubmed
,
Xenbase
Faulstich,
Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis.
1983,
Pubmed
Greengard,
Synaptic vesicle phosphoproteins and regulation of synaptic function.
1993,
Pubmed
Hackett,
Synapsin I injected presynaptically into goldfish mauthner axons reduces quantal synaptic transmission.
1990,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hirokawa,
The cytoskeletal architecture of the presynaptic terminal and molecular structure of synapsin 1.
1989,
Pubmed
Hurlbut,
Correlation between quantal secretion and vesicle loss at the frog neuromuscular junction.
1990,
Pubmed
Katz,
The role of calcium in neuromuscular facilitation.
1968,
Pubmed
Kelly,
Storage and release of neurotransmitters.
1993,
Pubmed
Koenig,
The relationship between the number of synaptic vesicles and the amount of transmitter released.
1989,
Pubmed
Lin,
Effects of synapsin I and calcium/calmodulin-dependent protein kinase II on spontaneous neurotransmitter release in the squid giant synapse.
1990,
Pubmed
Llinás,
Intraterminal injection of synapsin I or calcium/calmodulin-dependent protein kinase II alters neurotransmitter release at the squid giant synapse.
1985,
Pubmed
Llinás,
Regulation by synapsin I and Ca(2+)-calmodulin-dependent protein kinase II of the transmitter release in squid giant synapse.
1991,
Pubmed
Lo,
Activity-dependent synaptic competition in vitro: heterosynaptic suppression of developing synapses.
1991,
Pubmed
,
Xenbase
Lohof,
Potentiation of developing neuromuscular synapses by the neurotrophins NT-3 and BDNF.
1993,
Pubmed
,
Xenbase
Mallart,
The relation between quantum content and facilitation at the neuromuscular junction of the frog.
1968,
Pubmed
Matter,
Actin involvement in exocytosis from PC12 cells: studies on the influence of botulinum C2 toxin on stimulated noradrenaline release.
1989,
Pubmed
Nishida,
Characterization of the action of porcine brain profilin on actin polymerization.
1984,
Pubmed
Rodriguez Del Castillo,
Chromaffin cell scinderin, a novel calcium-dependent actin filament-severing protein.
1990,
Pubmed
Spitzer,
The development of the action potential mechanism of amphibian neurons isolated in culture.
1976,
Pubmed
,
Xenbase
Trifaró,
Cytoskeleton dynamics during neurotransmitter release.
1993,
Pubmed
Valtorta,
Structure and function of the synapsins.
1992,
Pubmed
Vitale,
Chromaffin cell cortical actin network dynamics control the size of the release-ready vesicle pool and the initial rate of exocytosis.
1995,
Pubmed
Wulf,
Fluorescent phallotoxin, a tool for the visualization of cellular actin.
1979,
Pubmed
Yin,
Identification of gelsolin, a Ca2+-dependent regulatory protein of actin gel-sol transformation, and its intracellular distribution in a variety of cells and tissues.
1981,
Pubmed
Zucker,
Short-term synaptic plasticity.
1989,
Pubmed
