XB-ART-1867Philos Trans R Soc Lond B Biol Sci March 29, 2005; 360 (1455): 591-4.
A comparison of the ability of XMAP215 and tau to inhibit the microtubule destabilizing activity of XKCM1.
During mitosis, microtubules not only grow fast, but also have a high rate of catastrophe. This is achieved in part by the activity of the MAP, XMAP215, which can stimulate the growth rate of microtubules without fully inhibiting the function of the catastrophe-kinesin XKCM1. We do not know whether this activity is particular to XMAP215, or is a general property of all MAPs. Here, we compare the activities of XMAP215 with the neuronal MAP tau, in opposing the destabilizing activity of the non-conventional kinesin XKCM1. We show that tau is a much more potent inhibitor of XKCM1 than XMAP215. Because tau completely suppresses XKCM1 activity, even at low concentrations, the combination of tau and XKCM1 is unable to generate mitotic microtubule dynamics.
PubMed ID: 15905143
PMC ID: PMC1569480
Article link: Philos Trans R Soc Lond B Biol Sci
Genes referenced: ckap5 kif2c mapt
References [+] :
Belmont, Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts. 1990, Pubmed, Xenbase