Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Multiple genes, tissue specificity, and expression-dependent modulationcontribute to the functional diversity of potassium channels in Arabidopsis thaliana.
Cao Y
,
Ward JM
,
Kelly WB
,
Ichida AM
,
Gaber RF
,
Anderson JA
,
Uozumi N
,
Schroeder JI
,
Crawford NM
.
???displayArticle.abstract???
K+ channels play diverse roles in mediating K+ transport and in modulating the membrane potential in higher plant cells during growth and development. Some of the diversity in K+ channel functions may arise from the regulated expression of multiple genes encoding different K+ channel polypeptides. Here we report the isolation of a novel Arabidopsis thaliana cDNA (AKT2) that is highly homologous to the two previously identified K+ channel genes, KAT1 and AKT1. This cDNA mapped to the center of chromosome 4 by restriction fragment length polymorphism analysis and was highly expressed in leaves, whereas AKT1 was mainly expressed in roots. In addition, we show that diversity in K+ channel function may be attributable to differences in expression levels. Increasing KAT1 expression in Xenopus oocytes by polyadenylation of the KAT1 mRNA increased the current amplitude and led to higher levels of KAT1 protein, as assayed in western blots. The increase in KAT1 expression in oocytes produced shifts in the threshold potential for activation to more positive membrane potentials and decreased half-activation times. These results suggest that different levels of expression and tissue-specific expression of different K+ channel isoforms can contribute to the functional diversity of plant K+ channels. The identification of a highly expressed, leaf-specific K+ channel homolog in plants should allow further molecular characterization of K+ channel functions for physiological K+ transport processes in leaves.
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992, Pubmed
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992,
Pubmed
Antonenko,
Ionic channel activity induced by fusion of Rhodospirillum rubrum chromatophores with a planar bilayer lipid membrane.
1994,
Pubmed
Baines,
Ankyrin and the node of Ranvier.
1990,
Pubmed
Bennett,
Ankyrins. Adaptors between diverse plasma membrane proteins and the cytoplasm.
1992,
Pubmed
Brown,
Functional bases for interpreting amino acid sequences of voltage-dependent K+ channels.
1993,
Pubmed
Cao,
Amino terminus and the first four membrane-spanning segments of the Arabidopsis K+ channel KAT1 confer inward-rectification property of plant-animal chimeric channels.
1995,
Pubmed
,
Xenbase
Dohmen,
An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera.
1991,
Pubmed
Drain,
Regulation of Shaker K+ channel inactivation gating by the cAMP-dependent protein kinase.
1994,
Pubmed
,
Xenbase
Elledge,
Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.
1991,
Pubmed
Elzenga,
Characterization of ion channels in the plasma membrane of epidermal cells of expanding pea (Pisum sativum arg) leaves.
1994,
Pubmed
Fairley-Grenot,
Evidence for G-Protein Regulation of Inward K+ Channel Current in Guard Cells of Fava Bean.
1991,
Pubmed
Gassmann,
Inward-Rectifying K+ Channels in Root Hairs of Wheat (A Mechanism for Aluminum-Sensitive Low-Affinity K+ Uptake and Membrane Potential Control).
1994,
Pubmed
Gilly,
Control of the spatial distribution of sodium channels in giant fiber lobe neurons of the squid.
1990,
Pubmed
Grimminger,
Suppression of cytoskeletal rearrangement in activated human neutrophils by botulinum C2 toxin. Impact on cellular signal transduction.
1991,
Pubmed
Hanley,
Plant intron sequences: evidence for distinct groups of introns.
1988,
Pubmed
Honoré,
Different types of K+ channel current are generated by different levels of a single mRNA.
1992,
Pubmed
,
Xenbase
Hoshi,
Regulation of voltage dependence of the KAT1 channel by intracellular factors.
1995,
Pubmed
,
Xenbase
Hymel,
Purified skeletal muscle 1,4-dihydropyridine receptor forms phosphorylation-dependent oligomeric calcium channels in planar bilayers.
1988,
Pubmed
Isacoff,
Evidence for the formation of heteromultimeric potassium channels in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Jan,
Structural elements involved in specific K+ channel functions.
1992,
Pubmed
Joshi,
An inspection of the domain between putative TATA box and translation start site in 79 plant genes.
1987,
Pubmed
Kaupp,
Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel.
1989,
Pubmed
,
Xenbase
Ko,
TRK1 and TRK2 encode structurally related K+ transporters in Saccharomyces cerevisiae.
1991,
Pubmed
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
Kubo,
Primary structure and functional expression of a mouse inward rectifier potassium channel.
1993,
Pubmed
,
Xenbase
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lew,
Electrogenic transport properties of growing Arabidopsis root hairs : the plasma membrane proton pump and potassium channels.
1991,
Pubmed
Milkman,
An Escherichia coli homologue of eukaryotic potassium channel proteins.
1994,
Pubmed
Moran,
Level of expression controls modes of gating of a K+ channel.
1992,
Pubmed
,
Xenbase
Peter,
The polarized distribution of poly(A+)-mRNA-induced functional ion channels in the Xenopus oocyte plasma membrane is prevented by anticytoskeletal drugs.
1991,
Pubmed
,
Xenbase
Ruppersberg,
Heteromultimeric channels formed by rat brain potassium-channel proteins.
1990,
Pubmed
,
Xenbase
Schachtman,
Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.
1992,
Pubmed
,
Xenbase
Schmidt,
Anion Selectivity of Slow Anion Channels in the Plasma Membrane of Guard Cells (Large Nitrate Permeability).
1994,
Pubmed
Schroeder,
Perspectives on the physiology and structure of inward-rectifying K+ channels in higher plants: biophysical implications for K+ uptake.
1994,
Pubmed
Schroeder,
Involvement of ion channels and active transport in osmoregulation and signaling of higher plant cells.
1989,
Pubmed
Schroeder,
Inward-rectifying K+ channels in guard cells provide a mechanism for low-affinity K+ uptake.
1991,
Pubmed
Schroeder,
Voltage dependence of K channels in guard-cell protoplasts.
1987,
Pubmed
Sentenac,
Cloning and expression in yeast of a plant potassium ion transport system.
1992,
Pubmed
Spalding,
Activation of K+ Channels in the Plasma Membrane of Arabidopsis by ATP Produced Photosynthetically.
1993,
Pubmed
Stühmer,
Structural parts involved in activation and inactivation of the sodium channel.
1989,
Pubmed
,
Xenbase
Sussman,
Shaking Arabidopsis thaliana.
1992,
Pubmed
Taleb,
Expression of the human glycine receptor alpha 1 subunit in Xenopus oocytes: apparent affinities of agonists increase at high receptor density.
1994,
Pubmed
,
Xenbase
Tempel,
Sequence of a probable potassium channel component encoded at Shaker locus of Drosophila.
1987,
Pubmed
Thiel,
Membrane transport in stomatal guard cells: the importance of voltage control.
1992,
Pubmed
Tsay,
The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter.
1993,
Pubmed
,
Xenbase
Uozumi,
Identification of strong modifications in cation selectivity in an Arabidopsis inward rectifying potassium channel by mutant selection in yeast.
1995,
Pubmed
,
Xenbase
Véry,
Level of expression in Xenopus oocytes affects some characteristics of a plant inward-rectifying voltage-gated K+ channel.
1994,
Pubmed
,
Xenbase
Véry,
Expression of a cloned plant K+ channel in Xenopus oocytes: analysis of macroscopic currents.
1995,
Pubmed
,
Xenbase
Ward,
Dissociation and Reassembly of the Vacuolar H-ATPase Complex from Oat Roots.
1992,
Pubmed
Warmke,
A distinct potassium channel polypeptide encoded by the Drosophila eag locus.
1991,
Pubmed
Weber,
Model of specific complex between catabolite gene activator protein and B-DNA suggested by electrostatic complementarity.
1984,
Pubmed
Wei,
K+ current diversity is produced by an extended gene family conserved in Drosophila and mouse.
1990,
Pubmed
,
Xenbase
Wilkinson,
Identification of the Arabidopsis CHL3 gene as the nitrate reductase structural gene NIA2.
1991,
Pubmed
