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Type 3 lodothyronine deiodinase: cloning, in vitro expression, and functional analysis of the placental selenoenzyme.
Salvatore D
,
Low SC
,
Berry M
,
Maia AL
,
Harney JW
,
Croteau W
,
St Germain DL
,
Larsen PR
.
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Type 3 iodothyronine deiodinase (D3) catalyzes the conversion of T4 and T3 to inactive metabolites. It is highly expressed in placenta and thus can regulate circulating fetal thyroid hormone concentrations throughout gestation. We have cloned and expressed a 2.1-kb human placental D3 cDNA which encodes a 32-kD protein with a Km of 1.2 nM for 5 deiodination of T3 and 340 nM for 5' deiodination of reverse T3. The reaction requires DTT and is not inhibited by 6n-propylthiouracil. We quantitated transiently expressed D3 by specifically labeling the protein with bromoacetyl [125I]T3. The Kcat/Km ratio for 5 deiodination of T3 was over 1,000-fold that for 5' deiodination of reverse T3. Human D3 is a selenoenzyme as evidenced by (a) the presence of an in frame UGA codon at position 144, (b) the synthesis of a 32-kD 75Se-labeled protein in D3 cDNA transfected cells, and (c) the presence of a selenocysteine insertion sequence element in the 3' untranslated region of the mRNA which is required for its expression. The D3 selenocysteine insertion sequence element is more potent than that in the type 1 deiodinase or glutathione peroxidase gene, suggesting a high priority for selenocysteine incorporation into this enzyme. The conservation of this enzyme from Xenopus laevis tadpoles to humans implies an essential role for regulation of thyroid hormone inactivation during embryological development.
Abuid,
Serum triiodothyronine and thyroxine in the neonate and the acute increases in these hormones following delivery.
1973, Pubmed
Abuid,
Serum triiodothyronine and thyroxine in the neonate and the acute increases in these hormones following delivery.
1973,
Pubmed
Arthur,
Hepatic iodothyronine 5'-deiodinase. The role of selenium.
1990,
Pubmed
Aruffo,
Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.
1987,
Pubmed
Axley,
Catalytic properties of an Escherichia coli formate dehydrogenase mutant in which sulfur replaces selenium.
1991,
Pubmed
Beckett,
Inhibition of type I and type II iodothyronine deiodinase activity in rat liver, kidney and brain produced by selenium deficiency.
1989,
Pubmed
Berry,
Type I iodothyronine deiodinase is a selenocysteine-containing enzyme.
1991,
Pubmed
,
Xenbase
Berry,
Selenocysteine confers the biochemical properties characteristic of the type I iodothyronine deiodinase.
1991,
Pubmed
,
Xenbase
Berry,
Recognition of UGA as a selenocysteine codon in type I deiodinase requires sequences in the 3' untranslated region.
1991,
Pubmed
Berry,
Functional characterization of the eukaryotic SECIS elements which direct selenocysteine insertion at UGA codons.
1993,
Pubmed
Berry,
Selenocysteine insertion or termination: factors affecting UGA codon fate and complementary anticodon:codon mutations.
1994,
Pubmed
Berry,
The role of selenium in thyroid hormone action.
1992,
Pubmed
Berry,
Substitution of cysteine for selenocysteine in type I iodothyronine deiodinase reduces the catalytic efficiency of the protein but enhances its translation.
1992,
Pubmed
Brent,
Functional characterization of the rat growth hormone promoter elements required for induction by thyroid hormone with and without a co-transfected beta type thyroid hormone receptor.
1989,
Pubmed
Brent,
Mutations of the rat growth hormone promoter which increase and decrease response to thyroid hormone define a consensus thyroid hormone response element.
1989,
Pubmed
Burrow,
Maternal and fetal thyroid function.
1994,
Pubmed
Chanoine,
The thyroid gland is a major source of circulating T3 in the rat.
1993,
Pubmed
Chanoine,
Placental 5-deiodinase activity and fetal thyroid hormone economy are unaffected by selenium deficiency in the rat.
1993,
Pubmed
Contempré,
Detection of thyroid hormones in human embryonic cavities during the first trimester of pregnancy.
1993,
Pubmed
Galton,
Hepatic iodothyronine 5-deiodinase activity in Rana catesbeiana tadpoles at different stages of the life cycle.
1987,
Pubmed
Gossen,
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
1992,
Pubmed
Kaplan,
Maturational patterns of iodothyronine phenolic and tyrosyl ring deiodinase activities in rat cerebrum, cerebellum, and hypothalamus.
1981,
Pubmed
Karmarkar,
5'-Monodeiodinase activity in developing human cerebral cortex.
1993,
Pubmed
Klein,
Effect of intraamniotic fluid thyroxine injection on fetal serum and amniotic fluid iodothyronine concentrations.
1978,
Pubmed
Larsen,
Relationships between circulating and intracellular thyroid hormones: physiological and clinical implications.
1981,
Pubmed
Larsen,
Type I iodothyronine deiodinase: unexpected complexities in a simple deiodination reaction.
1994,
Pubmed
Leinfelder,
In vitro synthesis of selenocysteinyl-tRNA(UCA) from seryl-tRNA(UCA): involvement and characterization of the selD gene product.
1990,
Pubmed
Leonard,
Localization of type I iodothyronine 5'-deiodinase to the basolateral plasma membrane in renal cortical epithelial cells.
1991,
Pubmed
Low,
Cloning and functional characterization of human selenophosphate synthetase, an essential component of selenoprotein synthesis.
1995,
Pubmed
Mandel,
Cloning and in vitro expression of the human selenoprotein, type I iodothyronine deiodinase.
1992,
Pubmed
Meinhold,
Effects of selenium and iodine deficiency on type I, type II and type III iodothyronine deiodinases and circulating thyroid hormones in the rat.
1993,
Pubmed
Mol,
Modification of rat liver iodothyronine 5'-deiodinase activity with diethylpyrocarbonate and rose bengal; evidence for an active site histidine residue.
1984,
Pubmed
Mori,
Thyroxine 5-deiodinase in human brain tumors.
1993,
Pubmed
Santini,
A study of the characteristics of the rat placental iodothyronine 5-monodeiodinase: evidence that it is distinct from the rat hepatic iodothyronine 5'-monodeiodinase.
1992,
Pubmed
Santini,
A study of metabolism of deaminated and sulfoconjugated iodothyronines by rat placental iodothyronine 5-monodeiodinase.
1992,
Pubmed
Schoenmakers,
Investigation of type I and type III iodothyronine deiodinases in rat tissues using N-bromoacetyl-iodothyronine affinity labels.
1995,
Pubmed
St Germain,
A thyroid hormone-regulated gene in Xenopus laevis encodes a type III iodothyronine 5-deiodinase.
1994,
Pubmed
,
Xenbase
Thorpe-Beeston,
Fetal thyroid function.
1992,
Pubmed
Toyoda,
Identification of critical amino acids for 3,5,3'-triiodothyronine deiodination by human type 1 deiodinase based on comparative functional-structural analyses of the human, dog, and rat enzymes.
1994,
Pubmed
Toyoda,
Topological analysis of the integral membrane protein, type 1 iodothyronine deiodinase (D1).
1995,
Pubmed