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The rat Na+-sulfate cotransporter rNaS2: functional characterization, tissue distribution, and gene (slc13a4) structure.
Dawson PA
,
Pirlo KJ
,
Steane SE
,
Nguyen KA
,
Kunzelmann K
,
Chien YJ
,
Markovich D
.
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Inorganic sulfate is essential for numerous functions in mammalian physiology. In the present study, we characterized the functional properties of the rat Na+-sulfate cotransporter NaS2 (rNaS2), determined its tissue distribution, and identified its gene (slc13a4) structure. Expression of rNaS2 protein in Xenopus oocytes led to a Na+-dependent transport of sulfate that was inhibited by phosphate, thiosulfate, tungstate, selenate, oxalate, and molybdate, but not by citrate, succinate, or DIDS. Transport kinetics of rNaS2 determined a K(M) for sulfate of 1.26 mM. Na+ kinetics determined a Hill coefficient of n=3.0+/-0.7, suggesting a Na+:SO4 (2-) stoichiometry of 3:1. rNaS2 mRNA was highly expressed in placenta, with lower levels found in the brain and liver. slc13a4 maps to rat chromosome 4 and contains 17 exons, spanning over 46 kb in length. This gene produces two alternatively spliced transcripts, of which the transcript lacking exon 2 is the most abundant form. Its 5' flanking region contains CAAT- and GC-box motifs and a number of putative transcription factor binding sites, including GATA-1, SP1, and AP-2 consensus sequences. This is the first study to characterize rNaS2 transport kinetics, define its tissue distribution, and resolve its gene (slc13a4) structure and 5' flanking region.
Beck,
The mouse Na(+)-sulfate cotransporter gene Nas1. Cloning, tissue distribution, gene structure, chromosomal assignment, and transcriptional regulation by vitamin D.
2000, Pubmed,
Xenbase
Beck,
The mouse Na(+)-sulfate cotransporter gene Nas1. Cloning, tissue distribution, gene structure, chromosomal assignment, and transcriptional regulation by vitamin D.
2000,
Pubmed
,
Xenbase
Bissig,
Functional expression cloning of the canalicular sulfate transport system of rat hepatocytes.
1994,
Pubmed
,
Xenbase
Bordukalo-Niksic,
Expression of brain and platelet serotonin transporters in sublines of rats with constitutionally altered serotonin homeostasis.
2004,
Pubmed
Busch,
Electrogenic cotransport of Na+ and sulfate in Xenopus oocytes expressing the cloned Na+SO4(2-) transport protein NaSi-1.
1994,
Pubmed
,
Xenbase
Dawson,
Impaired memory and olfactory performance in NaSi-1 sulphate transporter deficient mice.
2005,
Pubmed
Dawson,
Behavioural abnormalities of the hyposulphataemic Nas1 knock-out mouse.
2004,
Pubmed
Dawson,
Hyposulfatemia, growth retardation, reduced fertility, and seizures in mice lacking a functional NaSi-1 gene.
2003,
Pubmed
Dow,
Modulation of neurite promoting proteoglycans by neuronal differentiation.
1994,
Pubmed
Girard,
Molecular cloning and functional analysis of SUT-1, a sulfate transporter from human high endothelial venules.
1999,
Pubmed
,
Xenbase
Hästbacka,
The diastrophic dysplasia gene encodes a novel sulfate transporter: positional cloning by fine-structure linkage disequilibrium mapping.
1994,
Pubmed
Lee,
Identification of a mammalian brain sulfate transporter.
1999,
Pubmed
,
Xenbase
Lee,
The human renal sodium sulfate cotransporter (SLC13A1; hNaSi-1) cDNA and gene: organization, chromosomal localization, and functional characterization.
2000,
Pubmed
,
Xenbase
Lee,
NaSi-1 and Sat-1: structure, function and transcriptional regulation of two genes encoding renal proximal tubular sulfate transporters.
2005,
Pubmed
Lee,
The mouse sulfate anion transporter gene Sat1 (Slc26a1): cloning, tissue distribution, gene structure, functional characterization, and transcriptional regulation thyroid hormone.
2003,
Pubmed
,
Xenbase
Markovich,
Physiological roles and regulation of mammalian sulfate transporters.
2001,
Pubmed
Markovich,
Expression cloning of rat renal Na+/SO4(2-) cotransport.
1993,
Pubmed
,
Xenbase
Markovich,
Functional characterization and genomic organization of the human Na(+)-sulfate cotransporter hNaS2 gene (SLC13A4).
2005,
Pubmed
,
Xenbase
Markovich,
The SLC13 gene family of sodium sulphate/carboxylate cotransporters.
2004,
Pubmed
Quandt,
MatInd and MatInspector: new fast and versatile tools for detection of consensus matches in nucleotide sequence data.
1995,
Pubmed
Regeer,
Characterization of the human sulfate anion transporter (hsat-1) protein and gene (SAT1; SLC26A1).
2003,
Pubmed
,
Xenbase
Satoh,
Functional analysis of diastrophic dysplasia sulfate transporter. Its involvement in growth regulation of chondrocytes mediated by sulfated proteoglycans.
1998,
Pubmed
,
Xenbase
Strott,
Sulfonation and molecular action.
2002,
Pubmed
Tallgren,
Inorganic sulphates in relation to the serum thyroxine level and in renal failure.
1980,
Pubmed
