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XB-ART-19327
Development September 1, 1995; 121 (9): 3099-110.

PDGF signalling is required for gastrulation of Xenopus laevis.

Ataliotis P , Symes K , Chou MM , Ho L , Mercola M .


Abstract
During Xenopus gastrulation, platelet-derived growth factor (PDGF) receptor-alpha is expressed in involuting marginal zone cells which migrate over ectodermal cells expressing PDGF-A. To investigate the role of PDGF signalling during this process, we have generated a novel point mutant of PDGF receptor-alpha analogous to the W37 mutation of c-kit. This molecule is a specific, potent, dominant inhibitor of PDGF signalling in vivo. Injection of RNA encoding this protein into Xenopus embryos prevents closure of the blastopore, leads to abnormal gastrulation and a loss of anterior structures. Convergent extension is not inhibited in these embryos, but rather, involuting mesodermal cells fail to adhere to the overlying ectoderm. PDGF may therefore be required for mesodermal cell-substratum interaction.

PubMed ID: 7555734
Article link: Development
Grant support: [+]
Genes referenced: fgf2 gsc kit mrc1 pdgfa pdgfb pdgfra tbxt


Article Images: [+] show captions


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