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XB-ART-1954
Dev Cell May 1, 2005; 8 (5): 689-701.

FGF signal interpretation is directed by Sprouty and Spred proteins during mesoderm formation.

Sivak JM , Petersen LF , Amaya E .


Abstract
Vertebrate gastrulation requires coordination of mesoderm specification with morphogenetic movements. While both of these processes require FGF signaling, it is not known how mesoderm specification and cell movements are coordinated during gastrulation. The related Sprouty and Spred protein families are recently discovered regulators of receptor tyrosine kinase signaling. We identified two genes for each family in Xenopus tropicalis: Xtsprouty1, Xtsprouty2, Xtspred1, and Xtspred2. In gain- and loss-of-function experiments we show that XtSprouty and XtSpred proteins modulate different signaling pathways downstream of the FGF receptor (FGFR), and consequently different developmental processes. Notably, XtSproutys inhibit morphogenesis and Ca(2+) and PKCdelta signaling, leaving MAPK activation and mesoderm specification intact. In contrast, XtSpreds inhibit MAPK activation and mesoderm specification, with little effect on Ca(2+) or PKCdelta signaling. These differences, combined with the timing of their developmental expression, suggest a mechanism to switch FGFR signal interpretation to coordinate mesoderm formation and cell movements during gastrulation.

PubMed ID: 15866160
Article link: Dev Cell

Genes referenced: actc1 actl6a fgf2 fgfr1 fkbp1a kit map2k1 mapk1 prkcd spred1 spred2 spry1 spry2 tbxt vasp
Morpholinos: spred1 MO1 spred1 MO2 spred2 MO1 spred2 MO2 spry1 MO1 spry2 MO1 spry2 MO2


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