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XB-ART-203
PLoS Genet June 1, 2006; 2 (6): e91.

Genetic screens for mutations affecting development of Xenopus tropicalis.

Goda T , Abu-Daya A , Carruthers S , Clark MD , Stemple DL , Zimmerman LB .


Abstract
We present here the results of forward and reverse genetic screens for chemically-induced mutations in Xenopus tropicalis. In our forward genetic screen, we have uncovered 77 candidate phenotypes in diverse organogenesis and differentiation processes. Using a gynogenetic screen design, which minimizes time and husbandry space expenditures, we find that if a phenotype is detected in the gynogenetic F2 of a given F1 female twice, it is highly likely to be a heritable abnormality (29/29 cases). We have also demonstrated the feasibility of reverse genetic approaches for obtaining carriers of mutations in specific genes, and have directly determined an induced mutation rate by sequencing specific exons from a mutagenized population. The Xenopus system, with its well-understood embryology, fate map, and gain-of-function approaches, can now be coupled with efficient loss-of-function genetic strategies for vertebrate functional genomics and developmental genetics.

PubMed ID: 16789825
PMC ID: PMC1475704
Article link: PLoS Genet
Grant support: [+]
Genes referenced: actc1 actl6a alas2 atp12a b3gat1l btg3 cldn6.1 fgfr4 glul hba1 hba4 krt18.1 lama1 myh6 nfatc3 nipbl pck1 phf10 prdx2 rxrb sall4 smad4.2 sqstm1 tdrd6 trappc13 ube2d3 urod ventx1.1
Antibodies: B3gat1 Ab3 Lama1 Ab1


Article Images: [+] show captions
References:
Amaya, 1998, Pubmed, Xenbase [+]


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