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XB-ART-203
PLoS Genet. June 1, 2006; 2 (6): e91.

Genetic screens for mutations affecting development of Xenopus tropicalis.

Goda T , Abu-Daya A , Carruthers S , Clark MD , Stemple DL , Zimmerman LB .


Abstract
We present here the results of forward and reverse genetic screens for chemically-induced mutations in Xenopus tropicalis. In our forward genetic screen, we have uncovered 77 candidate phenotypes in diverse organogenesis and differentiation processes. Using a gynogenetic screen design, which minimizes time and husbandry space expenditures, we find that if a phenotype is detected in the gynogenetic F2 of a given F1 female twice, it is highly likely to be a heritable abnormality (29/29 cases). We have also demonstrated the feasibility of reverse genetic approaches for obtaining carriers of mutations in specific genes, and have directly determined an induced mutation rate by sequencing specific exons from a mutagenized population. The Xenopus system, with its well-understood embryology, fate map, and gain-of-function approaches, can now be coupled with efficient loss-of-function genetic strategies for vertebrate functional genomics and developmental genetics.

PubMed ID: 16789825
PMC ID: PMC1475704
Article link: PLoS Genet.
Grant support: 1 R01 HD4 2276-01 NICHD NIH HHSWellcome Trust , MC_U117560482 Medical Research Council , R01 HD042276 NICHD NIH HHS , 1 R01 HD4 2276-01 NICHD NIH HHSWellcome Trust , MRC_MC_U117560482 Medical Research Council , R01 HD042276 NICHD NIH HHS , 1 R01 HD4 2276-01 NICHD NIH HHSWellcome Trust , MC_U117560482 Medical Research Council , R01 HD042276 NICHD NIH HHS , 1 R01 HD4 2276-01 NICHD NIH HHSWellcome Trust

Genes referenced: actc1 actl6a alas2 atp12a btg3 cldn6.1 fgfr4 glul hba4 krt18 lama1 myh6 nfatc3 nipbl pck1 phf10 prdx2 rxrb sall4 sqstm1 trappc13 ube2d3 urod ventx1.1

Antibodies referenced: B3gat1 Ab3 Lama1 Ab1

References:
Amaya, 1998, Pubmed, Xenbase[+]


Article Images: [+] show captions

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