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Mol Cell Biol
1995 Jan 01;151:141-51. doi: 10.1128/MCB.15.1.141.
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Evidence for involvement of activin A and bone morphogenetic protein 4 in mammalian mesoderm and hematopoietic development.
Johansson BM
,
Wiles MV
.
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Xenopus in vitro studies have implicated both transforming growth factor beta (TGF-beta) and fibroblast growth factor (FGF) families in mesoderm induction. Although members of both families are present during mouse mesoderm formation, there is little evidence for their functional role in mesoderm induction. We show that mouse embryonic stem cells, which resemble primitive ectoderm, can differentiate to mesoderm in vitro in a chemically defined medium (CDM) in the absence of fetal bovine serum. In CDM, this differentiation is responsive to TGF-beta family members in a concentration-dependent manner, with activin A mediating the formation of dorsoanterior-like mesoderm and bone morphogenetic protein 4 mediating the formation of ventralmesoderm, including hematopoietic precursors. These effects are not observed in CDM alone or when TGF-beta 1, -beta 2, or -beta 3, acid FGF, or basic FGF is added individually to CDM. In vivo, at day 6.5 of mouse development, activin beta A RNA is detectable in the decidua and bone morphogenetic protein 4 RNA is detectable in the egg cylinder. Together, our data strongly implicate the TGF-beta family in mammalian mesoderm development and hematopoietic cell formation.
Adamson,
The localization and synthesis of some collagen types in developing mouse embryos.
1979, Pubmed
Adamson,
The localization and synthesis of some collagen types in developing mouse embryos.
1979,
Pubmed
Albano,
A mesoderm-inducing factor produced by WEHI-3 murine myelomonocytic leukemia cells is activin A.
1990,
Pubmed
,
Xenbase
Albano,
Activins are expressed in preimplantation mouse embryos and in ES and EC cells and are regulated on their differentiation.
1993,
Pubmed
Bastian,
A murine even-skipped homologue, Evx 1, is expressed during early embryogenesis and neurogenesis in a biphasic manner.
1990,
Pubmed
,
Xenbase
Biesecker,
Interleukin-6 is a component of human umbilical cord serum and stimulates hematopoiesis in embryonic stem cells in vitro.
1993,
Pubmed
Blum,
Gastrulation in the mouse: the role of the homeobox gene goosecoid.
1992,
Pubmed
,
Xenbase
Brotherton,
Hemoglobin ontogeny during normal mouse fetal development.
1979,
Pubmed
Burkert,
Early fetal hematopoietic development from in vitro differentiated embryonic stem cells.
1991,
Pubmed
Chinault,
Characterization and use of cloned sequences of the hypoxanthine-guanine phosphoribosyltransferase gene.
1984,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Cooke,
Growth-factor-related proteins that are inducers in early amphibian development may mediate similar steps in amniote (bird) embryogenesis.
1991,
Pubmed
,
Xenbase
Dale,
Bone morphogenetic protein 4: a ventralizing factor in early Xenopus development.
1992,
Pubmed
,
Xenbase
Dawid,
The role of growth factors in embryonic induction in Xenopus laevis.
1992,
Pubmed
,
Xenbase
Doetschman,
The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium.
1985,
Pubmed
Dush,
Analysis of mouse Evx genes: Evx-1 displays graded expression in the primitive streak.
1992,
Pubmed
Fisher,
Factors influencing the differentiation of embryonal carcinoma and embryo-derived stem cells.
1989,
Pubmed
Green,
The biological effects of XTC-MIF: quantitative comparison with Xenopus bFGF.
1990,
Pubmed
,
Xenbase
Heath,
Growth and differentiation factors of pluripotential stem cells.
1990,
Pubmed
Herrmann,
Cloning of the T gene required in mesoderm formation in the mouse.
1990,
Pubmed
Hoffmann,
Transforming growth factor-beta-related genes in Drosophila and vertebrate development.
1991,
Pubmed
,
Xenbase
Hébert,
mRNA localization studies suggest that murine FGF-5 plays a role in gastrulation.
1991,
Pubmed
Hébert,
Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis.
1990,
Pubmed
Isaacs,
Expression of a novel FGF in the Xenopus embryo. A new candidate inducing factor for mesoderm formation and anteroposterior specification.
1992,
Pubmed
,
Xenbase
Jahn,
DNA sequence organization of the beta-globin complex in the BALB/c mouse.
1980,
Pubmed
Jessell,
Diffusible factors in vertebrate embryonic induction.
1992,
Pubmed
Johnston,
Autoradiography using storage phosphor technology.
1990,
Pubmed
Jones,
Involvement of Bone Morphogenetic Protein-4 (BMP-4) and Vgr-1 in morphogenesis and neurogenesis in the mouse.
1991,
Pubmed
,
Xenbase
Jones,
DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
Kaiser,
The rat follistatin gene is highly expressed in decidual tissue.
1990,
Pubmed
Karasuyama,
Establishment of mouse cell lines which constitutively secrete large quantities of interleukin 2, 3, 4 or 5, using modified cDNA expression vectors.
1988,
Pubmed
Keller,
Hematopoietic commitment during embryonic stem cell differentiation in culture.
1993,
Pubmed
Kimelman,
Synergistic principles of development: overlapping patterning systems in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
Kokan-Moore,
Secretion of inhibin beta A by endoderm cultured from early embryonic chicken.
1991,
Pubmed
,
Xenbase
Köster,
Bone morphogenetic protein 4 (BMP-4), a member of the TGF-beta family, in early embryos of Xenopus laevis: analysis of mesoderm inducing activity.
1991,
Pubmed
,
Xenbase
Logan,
Induction of cardiac muscle differentiation in isolated animal pole explants of Xenopus laevis embryos.
1993,
Pubmed
,
Xenbase
Lyons,
Organogenesis and pattern formation in the mouse: RNA distribution patterns suggest a role for bone morphogenetic protein-2A (BMP-2A).
1990,
Pubmed
Lyons,
Vgr-1, a mammalian gene related to Xenopus Vg-1, is a member of the transforming growth factor beta gene superfamily.
1989,
Pubmed
,
Xenbase
Manova,
Expression of activins and TGF beta 1 and beta 2 RNAs in early postimplantation mouse embryos and uterine decidua.
1992,
Pubmed
Martin,
Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.
1981,
Pubmed
Maéno,
Regulation of primary erythropoiesis in the ventral mesoderm of Xenopus gastrula embryo: evidence for the expression of a stimulatory factor(s) in animal pole tissue.
1994,
Pubmed
,
Xenbase
Nagy,
Embryonic stem cells alone are able to support fetal development in the mouse.
1990,
Pubmed
Nagy,
Derivation of completely cell culture-derived mice from early-passage embryonic stem cells.
1993,
Pubmed
Oberbäumer,
Amino acid sequence of the non-collagenous globular domain (NC1) of the alpha 1(IV) chain of basement membrane collagen as derived from complementary DNA.
1985,
Pubmed
Paralkar,
Recombinant human bone morphogenetic protein 2B stimulates PC12 cell differentiation: potentiation and binding to type IV collagen.
1992,
Pubmed
Quinn-Laquer,
Characterization of the allelic differences in the mouse cardiac alpha-myosin heavy chain coding sequence.
1992,
Pubmed
Robbins,
Mouse embryonic stem cells express the cardiac myosin heavy chain genes during development in vitro.
1990,
Pubmed
Robertson,
Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector.
,
Pubmed
Rogers,
Bone morphogenetic proteins-2 and -4 are involved in the retinoic acid-induced differentiation of embryonal carcinoma cells.
1992,
Pubmed
Rogers,
Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes.
1991,
Pubmed
Rosen,
Whole-mount in situ hybridization in the mouse embryo: gene expression in three dimensions.
1993,
Pubmed
Sakai,
The measurement of activin/EDF in mouse serum: evidence for extragonadal production.
1992,
Pubmed
Schmitt,
Hematopoietic development of embryonic stem cells in vitro: cytokine and receptor gene expression.
1991,
Pubmed
Shen,
Leukemia inhibitory factor is expressed by the preimplantation uterus and selectively blocks primitive ectoderm formation in vitro.
1992,
Pubmed
Slack,
Embryonic induction.
1993,
Pubmed
,
Xenbase
Slack,
Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
,
Pubmed
,
Xenbase
Smith,
Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction.
1991,
Pubmed
,
Xenbase
Smith,
Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides.
1988,
Pubmed
Smith,
Identification of a potent Xenopus mesoderm-inducing factor as a homologue of activin A.
1990,
Pubmed
,
Xenbase
Sánchez,
Myosin heavy chain gene expression in mouse embryoid bodies. An in vitro developmental study.
1991,
Pubmed
Thomsen,
Activins are expressed early in Xenopus embryogenesis and can induce axial mesoderm and anterior structures.
1990,
Pubmed
,
Xenbase
Vainio,
Identification of BMP-4 as a signal mediating secondary induction between epithelial and mesenchymal tissues during early tooth development.
1993,
Pubmed
Wiles,
Embryonic stem cell differentiation in vitro.
1993,
Pubmed
Wiles,
Multiple hematopoietic lineages develop from embryonic stem (ES) cells in culture.
1991,
Pubmed
Wilkinson,
Expression pattern of the mouse T gene and its role in mesoderm formation.
1990,
Pubmed
,
Xenbase
Williams,
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells.
1988,
Pubmed
Wolpert,
Positional information and the spatial pattern of cellular differentiation.
1969,
Pubmed
Wong,
Properties of the earliest clonogenic hemopoietic precursors to appear in the developing murine yolk sac.
1986,
Pubmed
Wozney,
Bone morphogenetic proteins.
1989,
Pubmed
Wozney,
Novel regulators of bone formation: molecular clones and activities.
1988,
Pubmed
Zhou,
Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation.
1993,
Pubmed
,
Xenbase
van den Eijnden-Van Raaij,
Activin-like factor from a Xenopus laevis cell line responsible for mesoderm induction.
1990,
Pubmed
,
Xenbase
van den Eijnden-van Raaij,
Differentiation of aggregated murine P19 embryonal carcinoma cells is induced by a novel visceral endoderm-specific FGF-like factor and inhibited by activin A.
1991,
Pubmed
van den Eijnden-van Raaij,
Differential expression of inhibin subunits and follistatin, but not of activin receptor type II, during early murine embryonic development.
1992,
Pubmed
,
Xenbase
van der Kruijssen,
Modulation of activin expression by type beta transforming growth factors.
1993,
Pubmed
