XB-ART-20419Proc Natl Acad Sci U S A December 20, 1994; 91 (26): 12477-81.
Nuclear assembly is independent of linker histones.
The role of linker histones in the assembly of functional nuclei was examined with the use of a cell-free extract of Xenopus eggs that transforms condensed sperm chromatin into DNA-replication-competent pronuclei. When linker histones were removed from the extract, the resultant pronuclei were indistinguishable from those formed in the complete extract. The assembly of functional nuclear membrane, nuclear lamina, and prereplication centers allowed identical DNA replication efficiencies. Thus, linker histones are not required for the assembly of morphologically normal nuclei capable of DNA replication.
PubMed ID: 7809061
PMC ID: PMC45461
Article link: Proc Natl Acad Sci U S A
References [+] :
Blow, Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs. 1986, Pubmed, Xenbase