XB-ART-20449Cell December 2, 1994; 79 (5): 779-90.
A Xenopus gene whose expression can be activated by the organizer-specific homeobox genes goosecoid and Xnot2 was isolated by differential screening. The chordin gene encodes a novel protein of 941 amino acids that has a signal sequence and four Cys-rich domains. The expression of chordin starts in Spemann''s organizer subsequent to that of goosecoid, and its induction by activin requires de novo protein synthesis. Microinjection of chordin mRNA induces twinned axes and can completely rescue axial development in ventralized embryos. This molecule is a potent dorsalizing factor that is expressed at the right time and in the right place to regulate cell-cell interactions in the organizing centers of head, trunk, and tail development.
PubMed ID: 8001117
PMC ID: PMC3082463
Article link: Cell
Grant support: HD21502-09 NICHD NIH HHS , R37 HD021502-13 NICHD NIH HHS , R37 HD021502 NICHD NIH HHS
Genes referenced: chrd.1 gsc nog not
Article Images: [+] show captions
|Figure 1 chordin Is Expressed in Regions with Head, Trunk, and Tail Organizer Activity|
|Figure 2 chordin, but Not noggin, Expression Is Activated by gsc and Xnot2 Homeobox-Containing mRNAs|
|Figure 3 chordin Induction by Activin Requires De Novo Protein Synthesis|
|Figure 4 Xenopus chordin Encodes a Putative Secreted Protein|
|Figure 5 chordin mRNA Induces Secondary Axes|
|Figure 6 Dose-Dependent Axial Rescue by chordin mRNA|