XB-ART-20449
Cell
December 2, 1994;
79
(5):
779-90.
Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes.
Abstract
A Xenopus gene whose expression can be activated by the
organizer-specific homeobox genes
goosecoid and
Xnot2 was isolated by differential screening. The
chordin gene encodes a novel protein of 941 amino acids that has a signal sequence and four Cys-rich domains. The expression of
chordin starts in Spemann''s
organizer subsequent to that of
goosecoid, and its induction by activin requires de novo protein synthesis. Microinjection of
chordin mRNA induces twinned axes and can completely rescue axial development in ventralized embryos. This molecule is a potent dorsalizing factor that is expressed at the
right time and in the
right place to regulate cell-cell interactions in the organizing centers of
head,
trunk, and
tail development.
PubMed ID:
8001117
PMC ID:
PMC3082463
Article link:
Cell
Grant support:
[+]
Species referenced:
Xenopus laevis
Genes referenced:
chrd.1
gsc
nog
not
Article Images:
[+] show captions
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Figure 1
chordin Is Expressed in Regions with Head, Trunk, and Tail Organizer Activity
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Figure 2
chordin, but Not noggin, Expression Is Activated by gsc and Xnot2 Homeobox-Containing mRNAs
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Figure 3
chordin Induction by Activin Requires De Novo Protein Synthesis
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Figure 4
Xenopus chordin Encodes a Putative Secreted Protein
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Figure 5
chordin mRNA Induces Secondary Axes
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Figure 6
Dose-Dependent Axial Rescue by chordin mRNA
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