XB-ART-20612
EMBO J
November 1, 1994;
13
(21):
5015-25.
On the function of BMP-4 in patterning the marginal zone of the Xenopus embryo.
Abstract
Bone morphogenetic protein 4 (
BMP-4) is expressed in the
ventral marginal zone of the gastrulating
embryo. At late
gastrula stage this gene is expressed in the
ventral-most part of the slit
blastopore and in tissues that derive from it. At
tailbud stages
BMP-4 is expressed in the
spinal cord roof plate,
neural crest,
eye and auditory vesicle. The interactions of
BMP-4 with dorsal genes such as
goosecoid (
gsc) and
Xnot-2 were studied in vivo. In embryos ventralized by UV irradiation and suramin treatment,
BMP-4 zygotic transcripts accumulate prematurely and the entire
marginal zone expresses this gene. The patterning effect of
BMP-4 on ventro-
posterior development can be revealed by a sensitive assay involving the injection of
BMP-4 mRNA in the
ventral marginal zone of embryos partially dorsalized with LiCl, which leads to the complete rescue of
trunk and
tail structures. The experiments presented here argue that
BMP-4 may act in vivo as a
ventral signal for the proper patterning of the
marginal zone, actively interacting with dorsal genes such as
gsc and
Xnot-2. A model is proposed in which the timing of expression of various
marginal zone-specific genes plays a central role in patterning the
mesoderm.
PubMed ID:
7957067
PMC ID:
PMC395447
Article link:
Grant support:
[+]
Species referenced:
Xenopus laevis
Genes referenced:
bmp4
gsc
not
References [+] :
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed,
Xenbase
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Basler,
Control of cell pattern in the neural tube: regulation of cell differentiation by dorsalin-1, a novel TGF beta family member.
1993,
Pubmed
Blumberg,
Organizer-specific homeobox genes in Xenopus laevis embryos.
1991,
Pubmed
,
Xenbase
Chakrabarti,
Secretory and inductive properties of Drosophila wingless protein in Xenopus oocytes and embryos.
1992,
Pubmed
,
Xenbase
Cho,
Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid.
1992,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Christian,
Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus.
1993,
Pubmed
,
Xenbase
Christian,
Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing growth factors, may play a role in ventral mesodermal patterning during embryogenesis.
1991,
Pubmed
,
Xenbase
Dale,
Fate map for the 32-cell stage of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Dale,
Bone morphogenetic protein 4: a ventralizing factor in early Xenopus development.
1992,
Pubmed
,
Xenbase
Dirksen,
A novel, activin-inducible, blastopore lip-specific gene of Xenopus laevis contains a fork head DNA-binding domain.
1992,
Pubmed
,
Xenbase
Gerhart,
Cortical rotation of the Xenopus egg: consequences for the anteroposterior pattern of embryonic dorsal development.
1990,
Pubmed
,
Xenbase
Gilbert,
Spemann's organizer: models and molecules.
1993,
Pubmed
Gont,
Tail formation as a continuation of gastrulation: the multiple cell populations of the Xenopus tailbud derive from the late blastopore lip.
1994,
Pubmed
,
Xenbase
Grunz,
Suramin changes the fate of Spemann's organizer and prevents neural induction in Xenopus laevis.
1992,
Pubmed
,
Xenbase
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1977,
Pubmed
,
Xenbase
Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1992,
Pubmed
,
Xenbase
Jones,
DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
Jones,
Involvement of Bone Morphogenetic Protein-4 (BMP-4) and Vgr-1 in morphogenesis and neurogenesis in the mouse.
1991,
Pubmed
,
Xenbase
Kao,
The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos.
1988,
Pubmed
,
Xenbase
Kimelman,
Synergistic principles of development: overlapping patterning systems in Xenopus mesoderm induction.
1993,
Pubmed
,
Xenbase
Knöchel,
Activin A induced expression of a fork head related gene in posterior chordamesoderm (notochord) of Xenopus laevis embryos.
1992,
Pubmed
,
Xenbase
Ku,
Xwnt-11: a maternally expressed Xenopus wnt gene.
1994,
Pubmed
,
Xenbase
Köster,
Bone morphogenetic protein 4 (BMP-4), a member of the TGF-beta family, in early embryos of Xenopus laevis: analysis of mesoderm inducing activity.
1991,
Pubmed
,
Xenbase
Moody,
Fates of the blastomeres of the 32-cell-stage Xenopus embryo.
1987,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1983,
Pubmed
,
Xenbase
Niehrs,
Mesodermal patterning by a gradient of the vertebrate homeobox gene goosecoid.
1994,
Pubmed
,
Xenbase
Nishimatsu,
Genes for bone morphogenetic proteins are differentially transcribed in early amphibian embryos.
1992,
Pubmed
,
Xenbase
O'Keefe,
In situ hyridization.
1992,
Pubmed
,
Xenbase
Oschwald,
Suramin prevents transcription of dorsal marker genes in Xenopus laevis embryos, isolated dorsal blastopore lips and activin A induced animal caps.
1994,
Pubmed
,
Xenbase
Papkoff,
Secreted int-1 protein is associated with the cell surface.
1990,
Pubmed
Ruiz i Altaba,
Pintallavis, a gene expressed in the organizer and midline cells of frog embryos: involvement in the development of the neural axis.
1993,
Pubmed
,
Xenbase
Sasai,
Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split.
1993,
Pubmed
Scharf,
Determination of the dorsal-ventral axis in eggs of Xenopus laevis: complete rescue of uv-impaired eggs by oblique orientation before first cleavage.
1980,
Pubmed
,
Xenbase
Sive,
The frog prince-ss: a molecular formula for dorsoventral patterning in Xenopus.
1993,
Pubmed
,
Xenbase
Slack,
Embryonic induction.
1993,
Pubmed
,
Xenbase
Smith,
Mesoderm-inducing factors in early vertebrate development.
1993,
Pubmed
Smith,
Injected Xwnt-8 RNA acts early in Xenopus embryos to promote formation of a vegetal dorsalizing center.
1991,
Pubmed
,
Xenbase
Taira,
The LIM domain-containing homeo box gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos.
1992,
Pubmed
,
Xenbase
von Dassow,
Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box gene.
1993,
Pubmed
,
Xenbase