XB-ART-20612
EMBO J
November 1, 1994;
13
(21):
5015-25.
On the function of BMP-4 in patterning the marginal zone of the Xenopus embryo.
Abstract
Bone morphogenetic protein 4 (
BMP-4) is expressed in the
ventral marginal zone of the gastrulating
embryo. At late
gastrula stage this gene is expressed in the
ventral-most part of the slit
blastopore and in tissues that derive from it. At
tailbud stages
BMP-4 is expressed in the
spinal cord roof plate,
neural crest,
eye and auditory vesicle. The interactions of
BMP-4 with dorsal genes such as
goosecoid (
gsc) and
Xnot-2 were studied in vivo. In embryos ventralized by UV irradiation and suramin treatment,
BMP-4 zygotic transcripts accumulate prematurely and the entire
marginal zone expresses this gene. The patterning effect of
BMP-4 on ventro-
posterior development can be revealed by a sensitive assay involving the injection of
BMP-4 mRNA in the
ventral marginal zone of embryos partially dorsalized with LiCl, which leads to the complete rescue of
trunk and
tail structures. The experiments presented here argue that
BMP-4 may act in vivo as a
ventral signal for the proper patterning of the
marginal zone, actively interacting with dorsal genes such as
gsc and
Xnot-2. A model is proposed in which the timing of expression of various
marginal zone-specific genes plays a central role in patterning the
mesoderm.
PubMed ID:
7957067
PMC ID:
PMC395447
Article link:
Grant support:
[+]
Species referenced:
Xenopus laevis
Genes referenced:
bmp4
gsc
not
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