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XB-ART-20694
EMBO J
October 3, 1994;
13
(19):
4469-81.
eFGF regulates Xbra expression during Xenopus gastrulation.
Abstract
We show that, in addition to a role in
mesoderm induction during
blastula stages, FGF signalling plays an important role in maintaining the properties of the
mesoderm in the
gastrula of Xenopus laevis.
eFGF is a maternally expressed secreted Xenopus FGF with potent
mesoderm-inducing activity. However, it is most highly expressed in the
mesoderm during gastrulation, suggesting a role after the period of
mesoderm induction.
eFGF is inhibited by the dominant negative FGF receptor. Embryos overexpressing the dominant negative receptor show a change of behaviour of the dorsal
mesoderm such that it moves around the
blastopore lip instead of elongating in an antero-
posterior direction. In such embryos there is a reduction in
Xbra expression during gastrulation. We show that during
blastula stages
eFGF and
Xbra are able to activate the expression of each other, suggesting that they are components of an autocatalytic regulatory loop. Moreover, we show that
Xbra expression in isolated
gastrula mesoderm cells is maintained by
eFGF, suggesting that
eFGF continues to regulate the expression of
Xbra in the
blastopore region. In addition, overexpression of
eFGF after the mid-
blastula transition results in the up-regulation of
Xbra expression during
gastrula stages and causes suppression of the
head and enlargement of the
proctodeum, which is the converse of the
posterior reductions of the FGF dominant negative receptor phenotype. These data suggest an important role for
eFGF in regulating the expression of
Xbra and for the
eFGF-
Xbra regulatory pathway in the control of mesodermal cell behaviour during
gastrula stages.
PubMed ID:
7925289
PMC ID:
PMC395379
Article link:
Species referenced:
Xenopus laevis
Genes referenced:
fgf4
tbxt
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