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XB-ART-2071
Dev Cell April 1, 2005; 8 (4): 599-610.

Functional specificity of the Xenopus T-domain protein Brachyury is conferred by its ability to interact with Smad1.

Messenger NJ , Kabitschke C , Andrews R , Grimmer D , Núñez Miguel R , Blundell TL , Smith JC , Wardle FC .


Abstract
Members of the T-box gene family play important and diverse roles in development and disease. Here, we study the functional specificities of the Xenopus T-domain proteins Xbra and VegT, which differ in their abilities to induce gene expression in prospective ectodermal tissue. In particular, VegT induces strong expression of goosecoid whereas Xbra cannot. Our results indicate that Xbra is unable to induce goosecoid because it directly activates expression of Xom, a repressor of goosecoid that acts downstream of BMP signaling. We show that the inability of Xbra to induce goosecoid is imposed by an N-terminal domain that interacts with the C-terminal MH2 domain of Smad1, a component of the BMP signal transduction pathway. Interference with this interaction causes ectopic activation of goosecoid and anteriorization of the embryo. These findings suggest a mechanism by which individual T-domain proteins may interact with different partners to elicit a specific response.

PubMed ID: 15809041
Article link: Dev Cell

Genes referenced: ag1 agr2 gsc ncam1 otx2 smad1 smad2 smad4.1 smad4.2 tbxt vegt ventx2.2
Morpholinos: ventx2.1 MO4


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