Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1994 Jun 07;9112:5607-11.
Show Gene links
Show Anatomy links
Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter.
Ni B
,
Rosteck PR
,
Nadi NS
,
Paul SM
.
???displayArticle.abstract???
We have isolated a brain-specific cDNA that encodes a Na(+)-dependent inorganic phosphate (Pi) cotransporter (BNPI). The nucleotide sequence of BNPI predicts a protein of 560 amino acids with 6-8 putative transmembrane-spanning segments that is approximately 32% identical to the rabbit kidney Na(+)-dependent Pi cotransporter. Expression of BNPI mRNA in Xenopus oocytes results in Na(+)-dependent Pi transport similar to that reported for the recombinantly expressed or native kidney Na(+)-dependent cotransporter. RNA blot analysis reveals that BNPI mRNA is expressed predominantly (if not exclusively) in brain, and in situ hybridization histochemistry reveals BNPI transcripts in neurons of the cerebral cortex, hippocampus, and cerebellum. Furthermore, we have confirmed the presence of saturable Na(+)-dependent Pi cotransport in cultured cerebellar granule cells. Together, these data demonstrate the presence of a specific neuronal Na(+)-dependent transport system for Pi in brain.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Anner,
Uptake of orthophosphate by rabbit vagus nerve fibres.
1975,
Pubmed
Anner,
Sodium-dependent influx of orthophosphate in mammalian non-myelinated nerve.
1976,
Pubmed
Bikle,
The intestinal response to vitamin D.
1981,
Pubmed
Chuang,
N-methyl-D-aspartate exposure blocks glutamate toxicity in cultured cerebellar granule cells.
1992,
Pubmed
Deguchi,
Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B.
1990,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Ferreira,
Energy-linked anion transport. Cloning, sequencing, and characterization of a full length cDNA encoding the rat liver mitochondrial proton/phosphate symporter.
1989,
Pubmed
Fulceri,
Physiological concentrations of inorganic phosphate affect MgATP-dependent Ca2+ storage and inositol trisphosphate-induced Ca2+ efflux in microsomal vesicles from non-hepatic cells.
1993,
Pubmed
Gribskov,
Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins.
1986,
Pubmed
Himori,
Replenishment of brain adenosine triphosphate content by morphinan-type N-methyl-D-asparatate receptor antagonists, dextrorphan and dextromethorphan through the activation of adenylate kinase.
1992,
Pubmed
Kozak,
An analysis of vertebrate mRNA sequences: intimations of translational control.
1991,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Marini,
The neurotoxicity of 1-methyl-4-phenylpyridinium in cultured cerebellar granule cells.
1989,
Pubmed
Murer,
Cellular mechanisms in proximal tubular reabsorption of inorganic phosphate.
1991,
Pubmed
,
Xenbase
Nestler,
Protein phosphorylation in the brain.
,
Pubmed
Ni,
Molecular cloning of calmodulin mRNA species which are preferentially expressed in neurons in the rat brain.
1992,
Pubmed
Ni,
Developmental expression of neuronal calmodulin mRNA species in the rat brain analyzed by in situ hybridization.
1992,
Pubmed
Rosenmund,
Calcium-induced actin depolymerization reduces NMDA channel activity.
1993,
Pubmed
STRICKLER,
MICROPUNCTURE STUDY OF INORGANIC PHOSPHATE EXCRETION IN THE RAT.
1964,
Pubmed
Sulston,
The C. elegans genome sequencing project: a beginning.
1992,
Pubmed
Werner,
Expression of renal transport systems for inorganic phosphate and sulfate in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Werner,
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
1991,
Pubmed
,
Xenbase
