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J Virol
1994 May 01;685:3154-62. doi: 10.1128/JVI.68.5.3154-3162.1994.
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Identification and zinc dependence of the bovine herpesvirus 1 transactivator protein BICP0.
Fraefel C
,
Zeng J
,
Choffat Y
,
Engels M
,
Schwyzer M
,
Ackermann M
.
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Bovine herpesvirus 1 (BHV-1) specifies and unspliced early 2.6-kb RNA (ER2.6) which is 3' coterminal with exon 2 of the 2.9-kb immediate-early (IE) RNA. The two transcripts have a common open reading frame (676 codons). The predicted protein, designated BHV-1 infected cell protein 0 (BICP0), contains a zinc finger domain with homology to ICP0 of herpes simplex virus type 1 and protein 61 of varicella-zoster virus, and depending on the promoter, it acts as a strong activator or as a repressor in transient expression assays. In situ immunoadsorbent assays using antisera against synthetic oligopeptides demonstrated that BICP0 accumulates in nuclei of BHV-1-infected cells, as expected for an IE gene product involved in gene regulation. Western blots (immunoblots) revealed a BHV-1-specific 97-kDa protein which was detectable during the IE phase and also at later periods of infection, indicating that the kinetics of BICP0 synthesis is consistent with the switch from IER2.9 to ER2.6. To confirm that ER2.6 encoded the 97-kDa BICP0 protein, a DNA fragment containing BICP0-coding sequences was inserted into the Autographa californica baculovirus genome. A recombinant protein, identified by its reactivity with antipeptide sera, exhibited the same electrophoretic mobility as BICP0 specified by BHV-1. We microinjected Xenopus oocytes with a BICP0 effector plasmid and a promoter-chloramphenicol acetyltransferase plasmid. BICP0-induced stimulation of this promoter was strongly reduced when intracellular zinc was chelated by thionein, indicating that the effect of BICP0 is zinc dependent.
Ackermann,
Characterization of herpes simplex virus 1 alpha proteins 0, 4, and 27 with monoclonal antibodies.
1984, Pubmed
Ackermann,
Characterization of herpes simplex virus 1 alpha proteins 0, 4, and 27 with monoclonal antibodies.
1984,
Pubmed
Cai,
Herpes simplex virus type 1 ICP0 regulates expression of immediate-early, early, and late genes in productively infected cells.
1992,
Pubmed
Chen,
Mutational analysis of the sequence encoding ICP0 from herpes simplex virus type 1.
1991,
Pubmed
Chen,
Herpes simplex viruses with mutations in the gene encoding ICP0 are defective in gene expression.
1992,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Engels,
The genome of caprine herpesvirus 1: genome structure and relatedness to bovine herpesvirus 1.
1987,
Pubmed
Everett,
Analysis of the functional domains of herpes simplex virus type 1 immediate-early polypeptide Vmw110.
1988,
Pubmed
Everett,
Construction and characterization of herpes simplex type 1 viruses without introns in immediate early gene 1.
1991,
Pubmed
Everett,
A novel arrangement of zinc-binding residues and secondary structure in the C3HC4 motif of an alpha herpes virus protein family.
1993,
Pubmed
Everett,
Trans activation of transcription by herpes virus products: requirement for two HSV-1 immediate-early polypeptides for maximum activity.
1984,
Pubmed
Everett,
A detailed mutational analysis of Vmw110, a trans-acting transcriptional activator encoded by herpes simplex virus type 1.
1987,
Pubmed
Fraefel,
Immediate-early transcription over covalently joined genome ends of bovine herpesvirus 1: the circ gene.
1993,
Pubmed
Freedman,
The function and structure of the metal coordination sites within the glucocorticoid receptor DNA binding domain.
1988,
Pubmed
,
Xenbase
Freemont,
A novel cysteine-rich sequence motif.
1991,
Pubmed
Friedli,
Reactivity of monoclonal antibodies to proteins of a neurotropic bovine herpesvirus 1 (BHV-1) strain and to proteins of representative BHV-1 strains.
1987,
Pubmed
Johnston,
Autoradiography using storage phosphor technology.
1990,
Pubmed
KENDRICK,
Infectious pustular vulvovaginitis of cattle.
1958,
Pubmed
Kadonaga,
Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.
1987,
Pubmed
Kousoulas,
Mutations affecting conformation or sequence of neutralizing epitopes identified by reactivity of viable plaques segregate from syn and ts domains of HSV-1(F) gB gene.
1984,
Pubmed
Lovering,
Identification and preliminary characterization of a protein motif related to the zinc finger.
1993,
Pubmed
Metzler,
European isolates of bovine herpesvirus 1: a comparison of restriction endonuclease sites, polypeptides, and reactivity with monoclonal antibodies.
1985,
Pubmed
Metzler,
Bovine herpesvirus 1: molecular and antigenic characteristics of variant viruses isolated from calves with neurological disease.
1986,
Pubmed
Misra,
Proteins Specified by bovine herpesvirus 1 (infectious bovine rhinotracheitis virus).
1981,
Pubmed
Moriuchi,
Varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICP0.
1992,
Pubmed
Nagpal,
Characterization of a potent varicella-zoster virus-encoded trans-repressor.
1991,
Pubmed
Paterson,
Mutational dissection of the HSV-1 immediate-early protein Vmw175 involved in transcriptional transactivation and repression.
1988,
Pubmed
Pereira,
Regulation of herpesvirus macromolecular synthesis. V. Properties of alpha polypeptides made in HSV-1 and HSV-2 infected cells.
1977,
Pubmed
Schwyzer,
Promoter, spliced leader, and coding sequence for BICP4, the largest of the immediate-early proteins of bovine herpesvirus 1.
1993,
Pubmed
Stevenson,
Characterization of the varicella-zoster virus gene 61 protein.
1992,
Pubmed
Weber,
Identification of dominant-negative mutants of the herpes simplex virus type 1 immediate-early protein ICP0.
1992,
Pubmed
Wirth,
The three major immediate-early transcripts of bovine herpesvirus 1 arise from two divergent and spliced transcription units.
1991,
Pubmed
Wirth,
Immediate-early RNA 2.9 and early RNA 2.6 of bovine herpesvirus 1 are 3' coterminal and encode a putative zinc finger transactivator protein.
1992,
Pubmed
Zeng,
Thionein (apometallothionein) can modulate DNA binding and transcription activation by zinc finger containing factor Sp1.
1991,
Pubmed
