Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Potassium channels share a highly conserved stretch of eight amino acids, a K+ channel signature sequence. The conserved sequence falls within the previously defined P-region of voltage-activated K+ channels. In this study we investigate the effect of mutations in the signature sequence of the Shaker channel on K+ selectivity determined under bi-ionic conditions. Nonconservative substitutions of two threonine residues and the tyrosine residue leave selectivity intact. In contrast, mutations at some positions render the channel nonselective among monovalent cations. These findings are consistent with a proposal that the signature sequence contributes to a selectivity filter. Furthermore, the results illustrate that the hydroxyl groups at the third and fourth positions, and the aromatic group at position seven, are not essential in determining K+ selectivity.
Acharya,
Refined structure of baboon alpha-lactalbumin at 1.7 A resolution. Comparison with C-type lysozyme.
1989, Pubmed
Acharya,
Refined structure of baboon alpha-lactalbumin at 1.7 A resolution. Comparison with C-type lysozyme.
1989,
Pubmed
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992,
Pubmed
Atkinson,
A component of calcium-activated potassium channels encoded by the Drosophila slo locus.
1991,
Pubmed
Baumann,
Structure of the voltage-dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems.
1988,
Pubmed
Bezanilla,
Negative conductance caused by entry of sodium and cesium ions into the potassium channels of squid axons.
1972,
Pubmed
Bezanilla,
Inactivation of the sodium channel. I. Sodium current experiments.
1977,
Pubmed
Brüggemann,
Ether-à-go-go encodes a voltage-gated channel permeable to K+ and Ca2+ and modulated by cAMP.
1993,
Pubmed
,
Xenbase
Butler,
A family of putative potassium channel genes in Drosophila.
1989,
Pubmed
Butler,
mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels.
1993,
Pubmed
,
Xenbase
Choi,
The internal quaternary ammonium receptor site of Shaker potassium channels.
1993,
Pubmed
,
Xenbase
Frech,
A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.
1989,
Pubmed
,
Xenbase
Goldman,
POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANES.
1943,
Pubmed
HODGKIN,
The effect of sodium ions on the electrical activity of giant axon of the squid.
1949,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hartmann,
Exchange of conduction pathways between two related K+ channels.
1991,
Pubmed
,
Xenbase
Heginbotham,
A functional connection between the pores of distantly related ion channels as revealed by mutant K+ channels.
1992,
Pubmed
,
Xenbase
Heginbotham,
The aromatic binding site for tetraethylammonium ion on potassium channels.
1992,
Pubmed
Hille,
Potassium channels in myelinated nerve. Selective permeability to small cations.
1973,
Pubmed
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
Kamb,
Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity.
1988,
Pubmed
Kamb,
Molecular characterization of Shaker, a Drosophila gene that encodes a potassium channel.
1987,
Pubmed
Kavanaugh,
Interaction between tetraethylammonium and amino acid residues in the pore of cloned voltage-dependent potassium channels.
1991,
Pubmed
,
Xenbase
Kavanaugh,
Multiple subunits of a voltage-dependent potassium channel contribute to the binding site for tetraethylammonium.
1992,
Pubmed
,
Xenbase
Kim,
Crystal structure of human rhinovirus serotype 1A (HRV1A).
1989,
Pubmed
Kirsch,
Differences between the deep pores of K+ channels determined by an interacting pair of nonpolar amino acids.
1992,
Pubmed
,
Xenbase
Kubo,
Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel.
1993,
Pubmed
,
Xenbase
Kubo,
Primary structure and functional expression of a mouse inward rectifier potassium channel.
1993,
Pubmed
,
Xenbase
Kumpf,
A mechanism for ion selectivity in potassium channels: computational studies of cation-pi interactions.
1993,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Lopez,
Evidence that the S6 segment of the Shaker voltage-gated K+ channel comprises part of the pore.
1994,
Pubmed
,
Xenbase
MacKinnon,
Mutant potassium channels with altered binding of charybdotoxin, a pore-blocking peptide inhibitor.
1989,
Pubmed
,
Xenbase
MacKinnon,
Mapping the receptor site for charybdotoxin, a pore-blocking potassium channel inhibitor.
1990,
Pubmed
,
Xenbase
MacKinnon,
Charybdotoxin block of Shaker K+ channels suggests that different types of K+ channels share common structural features.
1988,
Pubmed
,
Xenbase
MacKinnon,
Mutations affecting TEA blockade and ion permeation in voltage-activated K+ channels.
1990,
Pubmed
Pak,
mShal, a subfamily of A-type K+ channel cloned from mammalian brain.
1991,
Pubmed
Pardo,
Extracellular K+ specifically modulates a rat brain K+ channel.
1992,
Pubmed
,
Xenbase
Perozo,
Gating currents from a nonconducting mutant reveal open-closed conformations in Shaker K+ channels.
1993,
Pubmed
Pongs,
Shaker encodes a family of putative potassium channel proteins in the nervous system of Drosophila.
1988,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schachtman,
Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.
1992,
Pubmed
,
Xenbase
Sentenac,
Cloning and expression in yeast of a plant potassium ion transport system.
1992,
Pubmed
Slesinger,
The S4-S5 loop contributes to the ion-selective pore of potassium channels.
1993,
Pubmed
,
Xenbase
Taglialatela,
Novel voltage clamp to record small, fast currents from ion channels expressed in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Tempel,
Sequence of a probable potassium channel component encoded at Shaker locus of Drosophila.
1987,
Pubmed
Toney,
Dialkylglycine decarboxylase structure: bifunctional active site and alkali metal sites.
1993,
Pubmed
Warmke,
A distinct potassium channel polypeptide encoded by the Drosophila eag locus.
1991,
Pubmed
Wei,
K+ current diversity is produced by an extended gene family conserved in Drosophila and mouse.
1990,
Pubmed
,
Xenbase
Yellen,
Mutations affecting internal TEA blockade identify the probable pore-forming region of a K+ channel.
1991,
Pubmed
Yokoyama,
Potassium channels from NG108-15 neuroblastoma-glioma hybrid cells. Primary structure and functional expression from cDNAs.
1989,
Pubmed
,
Xenbase
Yool,
Alteration of ionic selectivity of a K+ channel by mutation of the H5 region.
1991,
Pubmed
,
Xenbase
