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A role for histones H2A/H2B in chromatin folding and transcriptional repression.
Hansen JC
,
Wolffe AP
.
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Histone octamers or histone H3/H4 tetramers were reconstituted onto either closed circular plasmids containing a single Xenopus 5S rRNA gene or a reiterated array of Lytechinus 5S rRNA genes. All "reconstitutes" were found to undergo both Na(+)-dependent and Mg(2+)-dependent compaction. However, in each case, the compaction of nucleosomal templates containing H2A/H2B was much more extensive than compaction of templates containing only H3/H4 tetramers. Inclusion of 5 mM MgCl2 in the transcription buffer increased the level of compaction of nucleosomal templates and led to a marked inhibition of both transcription initiation and elongation by RNA polymerase III. The inhibitory effect of Mg2+ was reduced significantly when DNA templates contained only H3/H4 tetramers, consistent with their lesser extent of Mg(2+)-dependent compaction. Thus, the removal of histones H2A/H2B from nucleosomal arrays enhances gene activity, in part because of decreased levels of chromatin folding.
Almouzni,
Transcription complex disruption caused by a transition in chromatin structure.
1991, Pubmed,
Xenbase
Almouzni,
Transcription complex disruption caused by a transition in chromatin structure.
1991,
Pubmed
,
Xenbase
Baer,
Eukaryotic RNA polymerase II binds to nucleosome cores from transcribed genes.
1983,
Pubmed
Becker,
Heat shock-regulated transcription in vitro from a reconstituted chromatin template.
1991,
Pubmed
Camerini-Otero,
The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.
1976,
Pubmed
Chen,
Reversible and irreversible changes in nucleosome structure along the c-fos and c-myc oncogenes following inhibition of transcription.
1990,
Pubmed
Clark,
Electrostatic mechanism of chromatin folding.
1990,
Pubmed
Clark,
Superhelical stress and nucleosome-mediated repression of 5S RNA gene transcription in vitro.
1991,
Pubmed
,
Xenbase
Conconi,
Two different chromatin structures coexist in ribosomal RNA genes throughout the cell cycle.
1989,
Pubmed
De Bernardin,
Structure of in-vivo transcribing chromatin as studied in simian virus 40 minichromosomes.
1986,
Pubmed
Dong,
DNA and protein determinants of nucleosome positioning on sea urchin 5S rRNA gene sequences in vitro.
1990,
Pubmed
Dong,
Nucleosome positioning is determined by the (H3-H4)2 tetramer.
1991,
Pubmed
Ericsson,
Presence of histone H1 on an active Balbiani ring gene.
1990,
Pubmed
Garcia-Ramirez,
Role of the histone "tails" in the folding of oligonucleosomes depleted of histone H1.
1992,
Pubmed
González,
Interaction with RNA polymerase of nucleosomal cores lacking one H2A.H2B dimer.
1987,
Pubmed
González,
Interaction of RNA polymerase II with structurally altered nucleosomal particles. Transcription is facilitated by loss of one H2A.H2B dimer.
1989,
Pubmed
Hansen,
Chromatin dynamics and the modulation of genetic activity.
1992,
Pubmed
Hansen,
Influence of chromatin folding on transcription initiation and elongation by RNA polymerase III.
1992,
Pubmed
,
Xenbase
Hansen,
Assembly and structural properties of subsaturated chromatin arrays.
1993,
Pubmed
Hansen,
Homogeneous reconstituted oligonucleosomes, evidence for salt-dependent folding in the absence of histone H1.
1989,
Pubmed
Hansen,
The mechanism of nucleosome assembly onto oligomers of the sea urchin 5 S DNA positioning sequence.
1991,
Pubmed
Hayes,
The structure of DNA in a nucleosome.
1990,
Pubmed
,
Xenbase
Hayes,
Histone contributions to the structure of DNA in the nucleosome.
1991,
Pubmed
,
Xenbase
Hayes,
Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.
1992,
Pubmed
,
Xenbase
Jackson,
In vivo studies on the dynamics of histone-DNA interaction: evidence for nucleosome dissolution during replication and transcription and a low level of dissolution independent of both.
1990,
Pubmed
Knezetic,
The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro.
1986,
Pubmed
,
Xenbase
Lam,
Tandemly repeated DNA sequences from Xenopus laevis. I. Studies on sequence organization and variation in satellite 1 DNA (741 base-pair repeat).
1983,
Pubmed
,
Xenbase
Laybourn,
Role of nucleosomal cores and histone H1 in regulation of transcription by RNA polymerase II.
1991,
Pubmed
Lee,
A positive role for histone acetylation in transcription factor access to nucleosomal DNA.
1993,
Pubmed
,
Xenbase
Lee,
Transcription-induced nucleosome 'splitting': an underlying structure for DNase I sensitive chromatin.
1991,
Pubmed
Louters,
Exchange of histones H1, H2A, and H2B in vivo.
1985,
Pubmed
McKnight,
Post-replicative nonribosomal transcription units in D. melanogaster embryos.
1979,
Pubmed
Meersseman,
Chromatosome positioning on assembled long chromatin. Linker histones affect nucleosome placement on 5 S rDNA.
1991,
Pubmed
Nacheva,
Change in the pattern of histone binding to DNA upon transcriptional activation.
1989,
Pubmed
Noll,
Action of micrococcal nuclease on chromatin and the location of histone H1.
1977,
Pubmed
O'Neill,
Nucleosome arrays inhibit both initiation and elongation of transcripts by bacteriophage T7 RNA polymerase.
1992,
Pubmed
Pederson,
Effect of transcription of yeast chromatin on DNA topology in vivo.
1990,
Pubmed
Pruss,
Histone-DNA contacts in a nucleosome core containing a Xenopus 5S rRNA gene.
1993,
Pubmed
,
Xenbase
Schlissel,
The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1.
1984,
Pubmed
,
Xenbase
Shimamura,
Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Shimamura,
Histone H1 represses transcription from minichromosomes assembled in vitro.
1989,
Pubmed
,
Xenbase
Simon,
A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.
1979,
Pubmed
Simpson,
Structural features of a phased nucleosome core particle.
1983,
Pubmed
Simpson,
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.
1985,
Pubmed
Tremethick,
The transcription complex of the 5 S RNA gene, but not transcription factor IIIA alone, prevents nucleosomal repression of transcription.
1990,
Pubmed
,
Xenbase
Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase
Wolffe,
Dominant and specific repression of Xenopus oocyte 5S RNA genes and satellite I DNA by histone H1.
1989,
Pubmed
,
Xenbase
Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
Xenbase
van Holde,
What happens to nucleosomes during transcription?
1992,
Pubmed
