XB-ART-21520Mol Cell Biol March 1, 1994; 14 (3): 1893-900.
The deadenylation conferred by the 3'' untranslated region of a developmentally controlled mRNA in Xenopus embryos is switched to polyadenylation by deletion of a short sequence element.
The maternal Xenopus Eg mRNAs are adenylated and translated in the mature oocyte and then, after fertilization, are deadenylated and released from polysomes. Therefore, after fertilization, a change occurs in the cellular mechanisms that control mRNA adenylation. In the study reported here, we show that the 3'' untranslated region of Eg2 mRNA contains a cis-acting element that is required for the deadenylation of chimeric RNAs after fertilization. This cis-acting element is contained within a single 17-nucleotide portion of the Eg2 mRNA. Disruption of this deadenylation element allows adenylation of the chimeric transcripts in the embryo. Therefore, this cis-acting element is part of the sequence information required for the developmental switch from adenylation to deadenylation of the maternal Eg2 mRNA in Xenopus embryos.
PubMed ID: 8114721
PMC ID: PMC358547
Article link: Mol Cell Biol
Species referenced: Xenopus
Genes referenced: aurka
References [+] :
Bachvarova, A maternal tail of poly(A): the long and the short of it. 1992, Pubmed